Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HU754_RS03135 Genome accession   NZ_CP077090
Coordinates   710310..711374 (+) Length   354 a.a.
NCBI ID   WP_093430076.1    Uniprot ID   -
Organism   Pseudomonas zeae strain OE 48.2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 705310..716374
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HU754_RS03125 (HU754_003125) - 706034..707302 (+) 1269 WP_090280412.1 malic enzyme-like NAD(P)-binding protein -
  HU754_RS03130 (HU754_003130) - 707646..710093 (-) 2448 WP_186621296.1 penicillin-binding protein 1A -
  HU754_RS03135 (HU754_003135) pilM 710310..711374 (+) 1065 WP_093430076.1 pilus assembly protein PilM Machinery gene
  HU754_RS03140 (HU754_003140) - 711374..711940 (+) 567 WP_186621295.1 PilN domain-containing protein -
  HU754_RS03145 (HU754_003145) pilO 711937..712560 (+) 624 WP_099757776.1 type 4a pilus biogenesis protein PilO -
  HU754_RS03150 (HU754_003150) - 712557..713084 (+) 528 WP_186621293.1 pilus assembly protein PilP -
  HU754_RS03155 (HU754_003155) pilQ 713098..715179 (+) 2082 WP_186621291.1 type IV pilus secretin PilQ family protein Machinery gene
  HU754_RS03160 (HU754_003160) aroK 715184..715702 (+) 519 WP_011332081.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38107.72 Da        Isoelectric Point: 4.8109

>NTDB_id=579042 HU754_RS03135 WP_093430076.1 710310..711374(+) (pilM) [Pseudomonas zeae strain OE 48.2]
MLGLFNKKANTLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPVNAVVEKNIAELEGVGQALSRLLVKARTGLKSVAV
AVAGSAVITKIIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTL
EQAGLAKKQGGLPDDYISEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLSTPTQVANP
FANMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=579042 HU754_RS03135 WP_093430076.1 710310..711374(+) (pilM) [Pseudomonas zeae strain OE 48.2]
GTGCTGGGACTCTTCAATAAAAAGGCCAATACGTTACTGGGGATCGACATCAGTTCCACGTCAGTGAAGCTGCTGGAACT
GAGCCGTCAGGGTGATCGCTACCGGGTCGAGGCGTATGCGGTAGAGCCATTGCCAGTCAACGCCGTGGTCGAAAAGAACA
TCGCCGAGCTTGAAGGCGTCGGTCAGGCCCTGAGCCGTTTACTGGTCAAGGCGCGCACCGGGCTCAAGAGCGTGGCAGTC
GCAGTGGCCGGTTCTGCCGTGATCACCAAGATCATCGAGATGGACGCCGGGCTGTCCGACGACGAGCTGGAAAACCAGCT
CAAGATCGAGGCCGATCAGTACATTCCCTACCCGCTGGACGAAGTCGCCATCGATTTCGAAGTCCAGGGCGTGTCACCGC
GCAACCCTGAGCGGGTCAACGTGTTGCTCGCTGCCTGTCGCAAGGAAAACGTCGAAGTCCGCGAAGCGGCACTGGCTCTG
GCCGGGCTGACCGCACGGGTAGTCGACGTCGAAGCCTATGCGCTGGAGCGCTCGTTCGGCTTGCTGGCCACGCAACTGGC
GGCGTCTCAGGAGCGTCTGACTGTCGCTGTGGTCGACATCGGCGCGACCATGACCACGCTCAGCGTCCTGCACAATGGCA
AGATCATCTATACCCGCGAGCAATTGTTCGGCGGCCGGCAACTGACCGAGGAAATCCAGCGCCGCTATGGCCTGACCCTC
GAACAAGCGGGGCTGGCGAAAAAGCAGGGCGGTCTGCCCGATGATTACATCAGCGAGGTCTTGCAACCGTTTCGCGAGGC
GCTGGTGCAGCAAGTGTCGCGCTCGCTGCAGTTCTTCTTCGCCTCCGGGCAATACAACGCGGTCGATCACATTCTGCTGG
CCGGCGGCACGGCTTCAGTGCCCGGGCTGGATCGGCTGATCGAGCAGCGCCTGAGCACACCGACACAAGTAGCCAACCCG
TTTGCCAACATGGCCCTGAGCAGCAAGGTCAACGCCGGGGCTTTGGCCAGCGACGCGCCGGCGCTGATGATCGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.78

100

0.568
  comM Acinetobacter nosocomialis M2

56.78

100

0.568
  comM Acinetobacter baylyi ADP1

55.085

100

0.551
  pilM Legionella pneumophila strain ERS1305867

47.458

100

0.475