Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HU749_RS25410 Genome accession   NZ_CP077086
Coordinates   5758282..5759982 (+) Length   566 a.a.
NCBI ID   WP_046065032.1    Uniprot ID   -
Organism   Pseudomonas ogarae strain SWRI108     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5753282..5764982
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HU749_RS25390 (HU749_025390) - 5753400..5756333 (+) 2934 WP_186652350.1 DUF748 domain-containing protein -
  HU749_RS25395 (HU749_025395) - 5756335..5756625 (+) 291 WP_079301778.1 DUF2845 domain-containing protein -
  HU749_RS25400 (HU749_025400) - 5756953..5757306 (-) 354 WP_003205664.1 BON domain-containing protein -
  HU749_RS25405 (HU749_025405) - 5757626..5758057 (-) 432 WP_079301777.1 pilin -
  HU749_RS25410 (HU749_025410) pilB 5758282..5759982 (+) 1701 WP_046065032.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HU749_RS25415 (HU749_025415) pilC 5759985..5761202 (+) 1218 WP_079301776.1 type II secretion system F family protein Machinery gene
  HU749_RS25420 (HU749_025420) pilD 5761204..5762073 (+) 870 WP_079301775.1 prepilin peptidase Machinery gene
  HU749_RS25425 (HU749_025425) coaE 5762073..5762696 (+) 624 WP_186652354.1 dephospho-CoA kinase -
  HU749_RS25430 (HU749_025430) yacG 5762693..5762893 (+) 201 WP_041476151.1 DNA gyrase inhibitor YacG -
  HU749_RS25435 (HU749_025435) - 5762890..5763105 (-) 216 WP_013694234.1 hypothetical protein -
  HU749_RS25440 (HU749_025440) - 5763169..5763855 (-) 687 WP_079301773.1 energy-coupling factor ABC transporter permease -
  HU749_RS25445 (HU749_025445) - 5763897..5764307 (-) 411 WP_003205679.1 tautomerase family protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62761.76 Da        Isoelectric Point: 5.3203

>NTDB_id=578970 HU749_RS25410 WP_046065032.1 5758282..5759982(+) (pilB) [Pseudomonas ogarae strain SWRI108]
MTDIALSGLTKQLVLAELLTEQSAQQAYQQAQRSRIPLVSYLVQNKLVQSRQVAEIASEHFGVALLDLNSLDKETQPTGL
VSEKLVRQHHALPLWRRGNKLYVGISDPTNHQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFESSSTGLEGMGDVDLDG
LDIESIDDTRQDSIAGQDTDDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRVRTDGMLREVARPPIQLANRIAARL
KVMASLDISERRKPQDGRLKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKDLYMAALKQPQG
LILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLIRLQNMGIPGFNIATAVHLIIAQRLARKLCNHCKRAIEIPEETLLKEGFSR
ERIGTFTIYEPVGCEQCNQGYKGRVGVYEVVKNTPELQRLIMAEGNSLEIDLQMRKDGFNDLRASGLLKVMQGVTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=578970 HU749_RS25410 WP_046065032.1 5758282..5759982(+) (pilB) [Pseudomonas ogarae strain SWRI108]
ATGACTGACATCGCCCTCAGCGGCCTGACCAAGCAATTGGTGCTGGCCGAACTGCTCACCGAGCAAAGTGCGCAACAGGC
GTATCAACAGGCCCAACGCAGTCGCATTCCCCTGGTCAGTTATCTGGTGCAGAACAAACTGGTCCAGAGCCGCCAGGTCG
CGGAGATCGCCTCGGAGCATTTCGGCGTCGCCCTGCTGGACCTCAACAGCCTGGACAAAGAAACCCAACCCACCGGCCTG
GTCAGCGAAAAACTGGTGCGCCAGCACCACGCCCTGCCGCTGTGGCGGCGTGGCAACAAATTGTACGTAGGCATCTCCGA
CCCCACCAATCACCAGGCCATCAACGATATCCAGTTCAGCACCGGCCTGACTACCGAAGCCATCCTGGTGGAGGACGACA
AGCTCAGCGACGCCATCGAAAAGTTTTTCGAGTCCAGCAGCACCGGCCTGGAAGGCATGGGCGATGTCGACCTCGACGGC
CTGGACATCGAATCGATCGACGACACCAGGCAGGACTCCATCGCCGGCCAGGATACCGACGACGCGCCCGTGGTGCGCTT
CGTCAACAAGATGCTGCTGGACGCAATCAAGGGTGGCTCTTCCGACTTGCACTTCGAGCCCTATGAAAAGATCTATCGGG
TGCGGGTGCGCACCGACGGCATGCTGCGAGAAGTGGCCAGGCCGCCGATCCAACTGGCCAACCGTATCGCCGCACGCCTG
AAAGTCATGGCCAGCCTGGATATCTCCGAGCGGCGCAAACCCCAGGACGGGCGCCTGAAAATGCGCCTGTCGAAAACCAA
GTCCATCGATTTCCGGGTCAACACCCTGCCCACGCTCTGGGGCGAAAAAGTGGTGATCCGGATCCTCGACCCGTCCAGCG
CGCAAATGGGCATCGATGCCCTGGGCTACGAACCGGACCAGAAAGACCTGTACATGGCCGCCCTCAAGCAACCGCAAGGG
CTGATCCTGGTGACCGGCCCAACCGGCTCGGGCAAGACCGTGTCGCTGTACACCGGGCTGAATATCCTCAACACCGTGGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAATGTGAACCCGCGCCAGG
GGCTGGATTTCGCCCAGGCCCTGCGCTCGTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAAATCGCCATCAAGGCCGCCCAGACCGGGCACCTGGTGCTCTCCACACTGCATACCAACAGCGCCGCCGA
AACCCTGATCCGCCTGCAAAACATGGGCATTCCGGGGTTCAACATCGCCACCGCCGTGCACCTGATCATTGCCCAACGGC
TGGCGCGCAAGCTGTGCAACCACTGCAAGAGAGCCATCGAGATTCCCGAAGAAACCCTGCTCAAGGAAGGTTTCAGCCGG
GAACGCATCGGCACATTCACGATCTATGAGCCGGTCGGTTGCGAACAGTGCAACCAGGGTTACAAAGGGCGCGTGGGGGT
TTACGAAGTGGTCAAGAACACGCCCGAGCTGCAACGGTTGATCATGGCCGAGGGCAACTCGCTGGAAATCGATCTGCAGA
TGCGCAAGGACGGCTTCAATGACCTGCGCGCATCGGGGCTGCTCAAAGTGATGCAGGGCGTCACCAGCCTCGAAGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.304

100

0.583
  pilB Acinetobacter baumannii D1279779

57.443

100

0.58
  pilB Legionella pneumophila strain ERS1305867

55.556

100

0.557
  pilB Vibrio cholerae strain A1552

50.179

98.94

0.496
  pilB Vibrio campbellii strain DS40M4

48.596

100

0.489
  pilF Neisseria gonorrhoeae MS11

48.313

99.47

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

48.921

98.233

0.481
  pilF Thermus thermophilus HB27

36.219

100

0.362