Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KN217_RS02260 Genome accession   NZ_CP076348
Coordinates   485173..487248 (+) Length   691 a.a.
NCBI ID   WP_301619695.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain DN36     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 480173..492248
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KN217_RS02240 (KN217_02230) pilM 482337..483401 (+) 1065 WP_008568314.1 pilus assembly protein PilM Machinery gene
  KN217_RS02245 (KN217_02235) pilN 483401..483973 (+) 573 WP_301619694.1 type 4a pilus biogenesis protein PilN -
  KN217_RS02250 (KN217_02240) pilO 483970..484593 (+) 624 WP_213662533.1 type 4a pilus biogenesis protein PilO -
  KN217_RS02255 (KN217_02245) pilP 484590..485117 (+) 528 WP_008568310.1 type 4a pilus biogenesis lipoprotein PilP -
  KN217_RS02260 (KN217_02250) pilQ 485173..487248 (+) 2076 WP_301619695.1 type IV pilus secretin PilQ Machinery gene
  KN217_RS02265 (KN217_02255) aroK 487253..487798 (+) 546 WP_155551118.1 shikimate kinase AroK -
  KN217_RS02270 (KN217_02260) aroB 487819..488922 (+) 1104 WP_167136876.1 3-dehydroquinate synthase -
  KN217_RS02275 (KN217_02265) - 488932..490464 (+) 1533 WP_301619696.1 AAA family ATPase -

Sequence

Protein


Download         Length: 691 a.a.        Molecular weight: 74459.12 Da        Isoelectric Point: 4.5870

>NTDB_id=574804 KN217_RS02260 WP_301619695.1 485173..487248(+) (pilQ) [Stutzerimonas stutzeri strain DN36]
MNSTVSCLGVALLAFLAAPALLAANLNALDVAALPGDRVELKLAFDEPVNKPHGYTLEQPARIALDLPGVSNKLGVKNRE
LGVGNARSVTVVEAQGRTRLIVNLTTLVPYSTRIEGSNLFVVLGESSAPVAASNSTVAAPVATPAPSKPLLTSKAINNID
FRRGEDGAGNVIITLSDPSISSNIEEQGGKIRVSFAKTQLPESLRVRLDVQDFATPVKFVDATAQGGGAAVAIEPTGRYD
YLAYQTDDKLTISIKPLTEDEAQKRKAERFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQNVPW
DQALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELESQRQIAELAPLRREVVQVNYAKAADIAKLFQSVTNTEGQVD
ERGSIAVDDRTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGTQIFANGRGAVYGSDDF
GDEGGDAGEDSNGNYPFVDLGVTNRTAGIGIGYVTDNLILDLELSAMEKSGNGEIVSQPKVVTSDKETAKILKGSEIPYQ
EASSSGATSTSFKEAVLSLEVTPQITPDNRIIMEVKVTKDAPDYANALNDVPPINKNEVNAKVLVNDGETIVIGGVFSNT
QSRSVDKVPLLGDLPFLGRLFRRDSVTEEKSELLVFLTPRIVNNNAIAVSR

Nucleotide


Download         Length: 2076 bp        

>NTDB_id=574804 KN217_RS02260 WP_301619695.1 485173..487248(+) (pilQ) [Stutzerimonas stutzeri strain DN36]
ATGAATAGCACCGTCTCGTGTCTTGGTGTCGCATTGCTGGCGTTCTTGGCCGCCCCGGCTCTGCTGGCTGCCAACCTCAA
CGCCTTGGATGTTGCGGCGCTTCCCGGTGATCGTGTCGAGCTCAAGCTGGCGTTCGACGAGCCAGTGAATAAGCCGCATG
GCTATACGCTGGAGCAGCCGGCGCGCATTGCTCTGGATTTGCCCGGCGTGTCCAACAAGTTGGGTGTGAAGAATCGTGAG
TTGGGGGTAGGTAATGCTCGCAGCGTCACGGTTGTCGAGGCGCAAGGACGTACCCGTCTGATCGTCAATCTGACCACCCT
GGTGCCGTACTCGACGCGCATTGAGGGTAGCAACCTGTTCGTTGTGCTCGGCGAATCCTCGGCACCGGTTGCCGCATCCA
ATTCCACCGTCGCCGCCCCTGTAGCTACGCCGGCGCCTTCTAAACCGCTGCTAACGAGCAAGGCGATTAACAACATCGAT
TTTCGGCGCGGGGAGGATGGCGCTGGCAATGTGATCATCACGCTTTCCGACCCTTCGATCAGTTCGAATATCGAAGAGCA
GGGCGGGAAAATCCGCGTTTCGTTTGCCAAGACTCAGTTGCCTGAGTCTCTGCGGGTTCGTCTGGATGTGCAGGATTTCG
CTACTCCGGTGAAGTTTGTCGATGCCACCGCACAGGGTGGTGGTGCAGCTGTCGCCATTGAGCCTACAGGTCGCTACGAC
TATCTGGCCTATCAGACCGACGACAAGCTGACGATCAGCATCAAACCGCTGACCGAAGATGAGGCGCAGAAACGTAAGGC
GGAGCGATTTGCCTATTCGGGCGAGAAGTTGTCCCTCAACTTCCAGGACATCGATGTTCGCTCCGTACTGCAGTTGATTG
CAGACTTCACTGACCTCAATCTGGTCGCCAGCGATACGGTGCAGGGCAACATCACCCTGCGCCTGCAGAATGTGCCGTGG
GACCAGGCTCTTGATCTTGTCCTCAAGACCAAAGGGTTGGACAAGCGTCAGGTTGGTAATGTGCTGTTGGTCGCGCCTGC
TGACGAGATTGCTGCGCGCGAGCGCCAGGAACTCGAATCGCAGCGGCAGATCGCCGAGCTCGCTCCGCTGCGTCGGGAGG
TGGTACAGGTCAACTACGCGAAGGCAGCCGATATCGCCAAGCTCTTCCAGTCAGTGACCAACACCGAGGGTCAGGTGGAC
GAGCGTGGCTCCATTGCCGTTGATGATCGAACCAACAATATCATTGCCTATCAGACGCAGGATCGGCTGGACGAATTGCG
GCGTATCGTTGCGCAATTGGACATTCCGGTTCGTCAGGTGATGATCGAGGCGCGGATTGTTGAAGCTAACGTGGACTACG
ACAAGAGTCTGGGGGTCCGCTGGGGTGGCACCCAGATATTCGCTAACGGTCGCGGCGCAGTATACGGTAGCGACGACTTC
GGTGATGAGGGGGGCGACGCTGGCGAAGATAGTAACGGCAACTACCCGTTCGTCGATTTGGGGGTTACCAACCGTACCGC
AGGCATTGGTATCGGCTACGTGACTGACAACCTGATCCTTGATCTCGAACTTTCGGCTATGGAAAAGTCGGGTAATGGTG
AAATCGTTTCCCAGCCCAAGGTAGTGACCTCCGACAAAGAGACGGCGAAGATTCTCAAAGGATCTGAAATCCCCTATCAG
GAAGCCAGCTCCAGCGGAGCGACCAGTACGTCGTTCAAGGAGGCGGTGCTGTCGCTGGAAGTGACCCCGCAAATCACACC
GGACAATCGCATTATCATGGAAGTCAAGGTCACCAAGGATGCGCCCGACTATGCCAACGCGCTCAATGATGTTCCGCCTA
TTAATAAGAACGAGGTAAATGCCAAAGTACTGGTCAACGATGGTGAAACCATTGTCATCGGTGGCGTTTTCTCGAACACC
CAGAGCCGCTCAGTCGACAAGGTGCCGTTGCTGGGGGATCTGCCTTTCCTCGGTCGCCTGTTCCGTCGCGACTCCGTTAC
GGAGGAAAAATCGGAGCTGTTGGTCTTCCTTACTCCGCGTATAGTAAACAACAACGCTATAGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(39-122)

Secretin and TonB N terminus short domain (STN)

(300-346)

Bacterial type II and III secretion system protein (Secretin)

(526-682)

Bacterial type II/III secretion system short domain (Secretin_N)

(373-439)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.677

100

0.766
  comQ Acinetobacter baylyi ADP1

42.961

99.711

0.428
  pilQ Acinetobacter baumannii strain A118

39.281

100

0.411
  pilQ Acinetobacter baumannii D1279779

39.281

100

0.411
  pilQ Legionella pneumophila strain ERS1305867

38.496

98.119

0.378
  pilQ Legionella pneumophila strain Lp02

38.348

98.119

0.376