Detailed information NTG0574803

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: KN217_RS02240
• Genome accession: NZ_CP076348
• Coordinates: 482337..483401 (+)
• Length: 354 a.a.
• NCBI ID: WP_008568314.1
• Uniprot ID: -
• Organism: Stutzerimonas stutzeri strain DN36
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 477337..488401

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KN217_RS02225 (KN217_02215)	-	477475..478266 (+)	792	WP_301619691.1	thermonuclease family protein	-
KN217_RS02230 (KN217_02220)	-	478424..479692 (+)	1269	WP_301619692.1	malic enzyme-like NAD(P)-binding protein	-
KN217_RS02235 (KN217_02225)	-	479759..482158 (-)	2400	WP_301619693.1	penicillin-binding protein 1A	-
KN217_RS02240 (KN217_02230)	pilM	482337..483401 (+)	1065	WP_008568314.1	pilus assembly protein PilM	Machinery gene
KN217_RS02245 (KN217_02235)	pilN	483401..483973 (+)	573	WP_301619694.1	type 4a pilus biogenesis protein PilN	-
KN217_RS02250 (KN217_02240)	pilO	483970..484593 (+)	624	WP_213662533.1	type 4a pilus biogenesis protein PilO	-
KN217_RS02255 (KN217_02245)	pilP	484590..485117 (+)	528	WP_008568310.1	type 4a pilus biogenesis lipoprotein PilP	-
KN217_RS02260 (KN217_02250)	pilQ	485173..487248 (+)	2076	WP_301619695.1	type IV pilus secretin PilQ	Machinery gene
KN217_RS02265 (KN217_02255)	aroK	487253..487798 (+)	546	WP_155551118.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37940.41 Da
• Isoelectric Point: 4.6269

>NTDB_id=574803 KN217_RS02240 WP_008568314.1 482337..483401(+) (pilM) [Stutzerimonas stutzeri strain DN36]
MLGLFTKKANTLLGIDISSTSVKLLELSRSGNRYRVEAYAVEPLPPNAVVEKNIVELEGVGQALQRVVARAKVNGKQAAV
AVSGSAVITKSIEMESGLSDDELENQLKIEADQYIPYPLEEVAIDFEVQGPAARTPGRVEVLLAACRKENVEIREAALAL
AGLTAKVVDVEAYALERSYSLLAPMLAGAQGELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVSRSLQFFFAAGQFHDVDYILLAGGTASLPGLDRLIQQKIGTQTLVANP
FADMSLSNKVNAGALASDAPALMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=574803 KN217_RS02240 WP_008568314.1 482337..483401(+) (pilM) [Stutzerimonas stutzeri strain DN36]
GTGCTAGGGCTCTTCACGAAGAAAGCGAACACGCTACTTGGGATCGATATTAGTTCGACATCCGTCAAGCTCCTCGAATT
GAGCCGCTCGGGTAATCGCTATCGGGTGGAGGCTTACGCCGTTGAGCCGCTACCGCCGAATGCCGTCGTCGAGAAGAATA
TCGTCGAGCTCGAAGGCGTCGGTCAGGCGCTGCAGCGTGTTGTCGCCAGGGCCAAGGTGAATGGCAAGCAGGCGGCGGTT
GCCGTGTCTGGCTCGGCAGTCATCACCAAGAGCATCGAGATGGAGTCCGGCCTCTCGGACGATGAGTTGGAGAATCAGCT
CAAGATCGAGGCGGATCAGTACATCCCTTACCCCCTTGAAGAGGTCGCCATCGACTTCGAAGTACAGGGGCCTGCCGCGC
GTACGCCCGGACGTGTAGAGGTACTGCTGGCAGCCTGTCGCAAAGAAAACGTCGAAATTCGTGAGGCCGCCCTTGCACTG
GCGGGCCTCACTGCCAAGGTCGTGGATGTCGAGGCCTATGCGCTGGAGCGCTCCTATAGCCTGCTTGCTCCGATGTTGGC
AGGTGCGCAGGGCGAGCTGACGGTGGCTGTCGTTGATATCGGCGCCACCATGACGACGCTAAGCGTGTTGCACAACGGAC
GCACCATCTATACGCGCGAACAGCTATTCGGCGGCCGCCAGCTGACCGAAGAGATTCAGCGGCGTTATGGTTTGTCCGTA
GAGGAAGCTGGTTTGGCCAAGAAACAGGGCGGTCTTCCAGATGACTATGACAGCGAGGTGTTGCAGCCGTTCAAAGAGGC
GGTGGTGCAGCAGGTCTCGCGTTCGCTGCAATTCTTCTTCGCCGCCGGGCAATTCCATGATGTGGATTACATTTTGCTGG
CCGGTGGTACGGCGTCGCTGCCGGGACTCGATCGTCTGATCCAGCAGAAGATCGGTACGCAGACGCTAGTCGCCAATCCC
TTTGCCGATATGTCGCTGAGTAACAAGGTCAACGCTGGTGCGCTGGCCAGTGATGCACCGGCGCTGATGATTGCGTGTGG
CTTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.932	100	0.559
comM	Acinetobacter nosocomialis M2	55.932	100	0.559
comM	Acinetobacter baylyi ADP1	53.672	100	0.537
pilM	Legionella pneumophila strain ERS1305867	47.458	100	0.475