Detailed information NTG0573203

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: MO979_RS23855
• Genome accession: NZ_CP093965
• Coordinates: 5166364..5168064 (+)
• Length: 566 a.a.
• NCBI ID: WP_003107297.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain ATCC BAA-2108
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5161364..5173064

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MO979_RS23825 (MO979_23820)	nadC	5161930..5162778 (+)	849	WP_003116246.1	carboxylating nicotinate-nucleotide diphosphorylase	-
MO979_RS23835 (MO979_23830)	-	5162959..5164272 (-)	1314	WP_228480158.1	O-antigen ligase family protein	-
MO979_RS29505	-	5164430..5164786 (-)	357	WP_093945208.1	pilin	-
MO979_RS23845 (MO979_23840)	-	5164706..5165868 (+)	1163	WP_086937300.1	IS3-like element IS222 family transposase	-
MO979_RS23850 (MO979_23845)	-	5165882..5166133 (-)	252	Protein_4725	pilin	-
MO979_RS23855 (MO979_23850)	pilB	5166364..5168064 (+)	1701	WP_003107297.1	type IV-A pilus assembly ATPase PilB	Machinery gene
MO979_RS23860 (MO979_23855)	pilC	5168068..5169285 (+)	1218	WP_003161763.1	type II secretion system F family protein	Machinery gene
MO979_RS23865 (MO979_23860)	pilD	5169286..5170158 (+)	873	WP_023084740.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
MO979_RS23870 (MO979_23865)	coaE	5170155..5170766 (+)	612	WP_033982615.1	dephospho-CoA kinase	-
MO979_RS23875 (MO979_23870)	yacG	5170763..5170963 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
MO979_RS23880 (MO979_23875)	-	5171000..5171209 (-)	210	WP_003094660.1	hypothetical protein	-
MO979_RS23885 (MO979_23880)	-	5171315..5172004 (-)	690	WP_003103868.1	energy-coupling factor ABC transporter permease	-
MO979_RS23890 (MO979_23885)	-	5172001..5172471 (-)	471	WP_003094664.1	hypothetical protein	-
MO979_RS23895 (MO979_23890)	-	5172468..5172893 (-)	426	WP_003161766.1	GNAT family N-acetyltransferase	-

Sequence

Protein

• Length: 566 a.a.
• Molecular weight: 62312.21 Da
• Isoelectric Point: 5.4470

>NTDB_id=573203 MO979_RS23855 WP_003107297.1 5166364..5168064(+) (pilB) [Pseudomonas aeruginosa strain ATCC BAA-2108]
MNDSTPLSGLAKQLVLNELLDEKTARQAQVQAARNKQSLVSHLVQAKLVQSRALVEVAAEQFGIAYCDLNSLDRESIPKD
VISERLVRQHRVLPLWKRGNKLFIGLSDPTNHQAVTDIQFSTGLTTEALLVEDDKLGVAIEKLYEGAMDALGDLGDIDLD
GVDVGKAESASQDDGGDDAEDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKTYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKNKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
ELIGTFKLYSPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide

• Length: 1701 bp

>NTDB_id=573203 MO979_RS23855 WP_003107297.1 5166364..5168064(+) (pilB) [Pseudomonas aeruginosa strain ATCC BAA-2108]
ATGAACGATTCCACGCCTCTCAGCGGCCTTGCCAAGCAACTGGTCCTGAACGAACTCCTCGACGAAAAGACCGCCCGCCA
GGCCCAGGTGCAGGCCGCGCGCAACAAGCAGTCGCTGGTCAGCCACCTGGTCCAGGCCAAGCTGGTGCAGAGCCGGGCGC
TGGTCGAGGTGGCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGATCGGGAAAGCATTCCGAAGGAT
GTCATCAGCGAGCGCCTGGTCCGCCAGCACCGCGTGTTGCCGCTGTGGAAACGCGGCAACAAACTGTTCATCGGTCTCTC
CGACCCGACCAACCATCAGGCGGTCACCGATATCCAGTTCAGCACCGGCCTCACCACCGAAGCCCTGCTGGTAGAGGACG
ACAAGCTCGGCGTAGCCATCGAGAAGCTTTACGAGGGCGCCATGGACGCCCTCGGCGACCTCGGCGATATCGATCTCGAC
GGGGTCGATGTCGGCAAGGCGGAAAGCGCCAGCCAGGACGACGGCGGCGACGATGCCGAAGACGCCCCCGTGGTGCGCTT
CGTCAACAAGATGCTGCTCGACGCCATCCGCGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAAAAGACCTATCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGTATCTCTGCTCGTCTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGACGCAAACCGCAGGACGGACGGATCAAGATGCGCGTATCGAAGAACAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCACTGGGCTACGAAGAGGACCAGAAGGAACTCTACCTGGCCGCGCTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTTTCGCTGTACACCGGTCTGAACATCCTCAATACCACCGA
CATCAACATCTCCACTGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTG
GAGACTGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTCACCCGCCTGCTGAACATGGGTGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGACGTGCCGAAGGAAACCCTGCTTCACGAAGGTTTCCCGGAA
GAGCTGATCGGCACCTTCAAGCTGTATTCGCCTGTAGGCTGCGAAAACTGCAAAGGCGGCTACAAAGGTCGCGTCGGTAT
TTATGAAGTGGTTAAAAACACCCCCGCCCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGAATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (190-457)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-146)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	57.544	100	0.58
pilB	Acinetobacter baylyi ADP1	56.79	100	0.569
pilB	Legionella pneumophila strain ERS1305867	54.497	100	0.546
pilB	Vibrio cholerae strain A1552	51.521	98.763	0.509
pilF	Neisseria gonorrhoeae MS11	50.177	99.647	0.5
pilB	Vibrio parahaemolyticus RIMD 2210633	52.158	94.17	0.491
pilB	Vibrio campbellii strain DS40M4	51.22	94.17	0.482
pilB/pilB1	Synechocystis sp. PCC 6803	37.763	100	0.412
pilF	Thermus thermophilus HB27	38.664	100	0.389
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.727	97.173	0.376