Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KME82_RS20105 Genome accession   NZ_CP076103
Coordinates   4833137..4834864 (+) Length   575 a.a.
NCBI ID   WP_215495584.1    Uniprot ID   -
Organism   Lysobacter capsici strain NF87_2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4828137..4839864
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KME82_RS20080 (KME82_20080) - 4828459..4829349 (-) 891 WP_215495579.1 hypothetical protein -
  KME82_RS20085 (KME82_20085) - 4829526..4830206 (-) 681 WP_215495580.1 class I SAM-dependent methyltransferase -
  KME82_RS20090 (KME82_20090) - 4830203..4831048 (-) 846 WP_215495581.1 hypothetical protein -
  KME82_RS20095 (KME82_20095) - 4831045..4832097 (-) 1053 WP_215495582.1 UDP-N-acetylglucosamine 2-epimerase -
  KME82_RS20100 (KME82_20100) - 4832150..4833001 (-) 852 WP_215495583.1 glycosyltransferase family 2 protein -
  KME82_RS20105 (KME82_20105) pilB 4833137..4834864 (+) 1728 WP_215495584.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KME82_RS20110 (KME82_20110) pilC 4834868..4836124 (+) 1257 WP_215495585.1 type II secretion system F family protein Machinery gene
  KME82_RS20115 (KME82_20115) - 4836146..4837033 (+) 888 WP_215495586.1 A24 family peptidase -
  KME82_RS20120 (KME82_20120) coaE 4837037..4837657 (+) 621 WP_215495587.1 dephospho-CoA kinase -
  KME82_RS20125 (KME82_20125) - 4837759..4838742 (-) 984 WP_252255846.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62830.31 Da        Isoelectric Point: 6.3665

>NTDB_id=571845 KME82_RS20105 WP_215495584.1 4833137..4834864(+) (pilB) [Lysobacter capsici strain NF87_2]
MSTVATANLVGITGIARRLVMDGALDEVKAREAMTAASAERKPLASYLAEHRLATSAQLAAANSIEFGVPLFDPTTMDPG
QAPIRAISEELIRKHNALPLFKRGNKLFIGLADPTNTKALDEIKFQTNAAVEAILVDEELIRRTLDRWLETSDALGDAVG
DGEGLDNLEIGKDDDLSTTSDTGIDAKGDDTPVVKFINKVLVDAIRRGASDIHFEPYETDYRVRLRIDGLLKQVAKVPNK
LHPRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRMSTLPTLFGEKIVLRILDGSAARLGIEKLGYEDYQKELF
VNAVVKPYGMVLVTGPTGSGKTVSLYTALNILNDEQRNISTVEDPVEIRVPGINQVQMNVKRGMTFAAALRSFLRQDPDV
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGVAPYNITSSVTLVIAQRLARRLHDCKREVHLPEHA
LLAEGFTHDEIASGFKLYEPVGCPDCTEGYKGRTGIYQVMPMTDEIQAIVLEGGNAMQIAAAAIRSGVPDLRRSALVKVM
NGVTGLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=571845 KME82_RS20105 WP_215495584.1 4833137..4834864(+) (pilB) [Lysobacter capsici strain NF87_2]
ATGTCCACTGTCGCGACCGCCAACTTGGTGGGAATCACCGGCATTGCCCGGCGTTTGGTCATGGATGGGGCTCTGGACGA
GGTCAAGGCGCGCGAAGCCATGACCGCCGCCAGCGCCGAACGCAAGCCGCTGGCCTCCTACCTGGCCGAACACCGCCTGG
CCACCTCGGCCCAGCTCGCCGCCGCCAACTCGATCGAGTTCGGCGTGCCGCTGTTCGACCCGACCACCATGGACCCGGGC
CAGGCCCCGATCCGGGCGATCAGCGAAGAGCTGATCCGCAAGCACAACGCCTTGCCGCTGTTCAAGCGCGGCAACAAATT
GTTTATCGGCCTGGCCGACCCGACCAACACCAAGGCGCTGGACGAGATCAAGTTCCAGACCAACGCCGCGGTCGAGGCGA
TCTTGGTCGATGAGGAACTGATCCGCCGCACCCTGGACCGCTGGCTGGAAACCTCCGACGCCCTCGGCGACGCGGTCGGC
GACGGCGAGGGTCTGGACAACCTGGAGATCGGCAAGGACGACGACCTCAGCACCACCAGCGACACCGGCATCGACGCCAA
GGGCGACGACACTCCGGTGGTCAAGTTCATCAACAAGGTGCTGGTCGACGCGATCCGTCGCGGCGCGTCCGACATCCACT
TCGAACCCTACGAAACCGACTACCGGGTGCGCCTGCGCATCGACGGCCTGCTCAAGCAGGTCGCCAAGGTCCCGAACAAG
CTGCACCCGCGCATCGCCGCGCGCCTGAAGGTCATGGCGCAGCTCGACATCGCCGAGAAGCGGGTGCCGCAGGACGGACG
CATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCATGAGCACCTTGCCGACCCTGTTCGGCGAGAAGATCG
TGCTGCGTATCCTCGACGGCAGCGCGGCGCGGCTGGGCATCGAAAAGCTCGGTTACGAGGACTACCAGAAGGAATTGTTC
GTCAACGCGGTGGTCAAGCCCTACGGCATGGTGCTGGTCACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTGTACAC
CGCGCTCAACATCCTCAACGACGAACAGCGCAACATCTCCACGGTCGAGGACCCGGTCGAAATCCGCGTGCCGGGCATCA
ACCAGGTGCAGATGAACGTCAAGCGCGGCATGACCTTCGCCGCCGCGCTGCGCAGCTTCCTGCGCCAGGACCCGGACGTG
ATCATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGAGATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGCTGTC
GACCCTGCACACCAACGACGCGCCGCAGACCATCGCCCGCCTGATGAACATGGGCGTGGCACCGTACAACATCACCTCGT
CGGTCACCCTGGTGATCGCGCAGCGCCTCGCCCGCCGTCTGCACGACTGCAAGCGCGAGGTGCATCTGCCCGAACACGCG
CTGCTCGCCGAGGGGTTCACCCACGACGAGATCGCCTCGGGATTCAAGTTGTACGAGCCGGTCGGATGTCCGGACTGCAC
CGAAGGCTACAAGGGACGTACCGGCATCTATCAGGTCATGCCCATGACCGACGAAATCCAGGCCATCGTGCTCGAAGGCG
GCAATGCGATGCAGATCGCCGCCGCGGCGATCCGATCGGGCGTGCCCGATCTGCGTCGCTCCGCGCTGGTCAAGGTGATG
AACGGCGTCACCGGCCTGGCCGAAATCAACCGCGTAACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(199-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.185

98.957

0.546
  pilB Acinetobacter baylyi ADP1

55.203

98.609

0.544
  pilB Legionella pneumophila strain ERS1305867

50.438

99.304

0.501
  pilB Vibrio cholerae strain A1552

49.04

99.652

0.489
  pilF Neisseria gonorrhoeae MS11

47.266

98.609

0.466
  pilB Vibrio parahaemolyticus RIMD 2210633

49.617

90.783

0.45
  pilB Vibrio campbellii strain DS40M4

51.534

85.043

0.438
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.074

100

0.374
  pilF Thermus thermophilus HB27

39.734

91.478

0.363
  pilB/pilB1 Synechocystis sp. PCC 6803

37.41

96.696

0.362