Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KKZ03_RS17615 Genome accession   NZ_CP076024
Coordinates   3888944..3890656 (+) Length   570 a.a.
NCBI ID   WP_243221668.1    Uniprot ID   -
Organism   Methylobacter sp. S3L5C     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3883944..3895656
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KKZ03_RS17610 (KKZ03_17530) cas3 3885453..3888155 (-) 2703 WP_243218088.1 CRISPR-associated helicase Cas3' -
  KKZ03_RS17615 (KKZ03_17535) pilB 3888944..3890656 (+) 1713 WP_243221668.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KKZ03_RS17620 (KKZ03_17540) pilC 3890659..3891879 (+) 1221 WP_243218089.1 type II secretion system F family protein Machinery gene
  KKZ03_RS17625 (KKZ03_17545) pilD 3891949..3892830 (+) 882 WP_243218090.1 A24 family peptidase Machinery gene
  KKZ03_RS17630 (KKZ03_17550) coaE 3892835..3893452 (+) 618 WP_243218091.1 dephospho-CoA kinase -
  KKZ03_RS17635 (KKZ03_17555) zapD 3893445..3894215 (+) 771 WP_243218092.1 cell division protein ZapD -
  KKZ03_RS17640 (KKZ03_17560) yacG 3894215..3894421 (+) 207 WP_243218093.1 DNA gyrase inhibitor YacG -
  KKZ03_RS17645 (KKZ03_17565) - 3894502..3895062 (-) 561 WP_243218094.1 hypothetical protein -
  KKZ03_RS17650 (KKZ03_17570) - 3895066..3895590 (-) 525 WP_243218095.1 hypothetical protein -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63154.02 Da        Isoelectric Point: 5.3110

>NTDB_id=571357 KKZ03_RS17615 WP_243221668.1 3888944..3890656(+) (pilB) [Methylobacter sp. S3L5C]
MPTAPTDFQLSGFIKFLILEGLLTESEAKIHNQEAQKNNLPLLSYLAASHLVDSKVVATKASNEYGVSFLDLDALDLHSL
PISLINEKLIRKHRILPVFMRGKILFIAQSDPTNLLALDEIKFQSRLHPETILVEENKLAKAIEIALEVVDTSMTDLLDE
DLDNLDISGGDQESTKTDIDTDIDDAPIVRFVNKILLDAIKKGASDIHMEPYEKNFRIRFRADGILYQVASPPTNIASRI
ISRVKVMAKMDIAERRVPQDGRIKMNLSKHLAIDFRVNSCPTLFGEKVVLRILDPTSAQIGIEKLGFEPEQEKLFLHAIN
KPYGMVLVTGPTGSGKTVTLYTGLNLLNSIERNICTAEDPVEITVEGINQVNMNVKAGLTFASALRAFLRQDPDVIMVGE
IRDLETADIAVKAAQTGHLVLSTLHTNDAPQTLNRLMQMGIEPFNIVSAVILIMAQRLARRLCEYCKIPADFPEHILVSA
GFTEDELKTLKIYSSVGCEHCTNGYKGRVGIYQVMTLSENMRALILEGGNAMQLAEQAKAEGINDLRASGLNKVRMGITS
LEEIDRVTKE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=571357 KKZ03_RS17615 WP_243221668.1 3888944..3890656(+) (pilB) [Methylobacter sp. S3L5C]
ATGCCCACTGCACCCACAGATTTTCAGCTTAGTGGTTTTATAAAGTTCTTAATTCTGGAAGGTTTGCTTACGGAAAGTGA
AGCCAAAATTCATAACCAGGAAGCACAAAAAAACAATCTCCCCTTATTAAGCTATCTTGCTGCAAGTCATCTTGTTGACA
GTAAAGTGGTCGCAACAAAAGCCTCAAATGAATACGGTGTATCCTTTCTTGATCTCGATGCTCTTGATCTCCATAGTTTG
CCAATTAGTCTAATCAACGAAAAACTGATCCGTAAACATCGCATTTTGCCAGTGTTTATGCGGGGAAAAATACTATTTAT
AGCCCAATCTGATCCAACCAACCTTTTGGCGTTGGATGAAATAAAATTTCAAAGTCGGCTACATCCAGAAACCATTCTGG
TCGAAGAAAACAAGCTGGCCAAGGCTATTGAAATTGCCCTGGAAGTCGTTGATACCTCAATGACTGATCTTCTTGACGAA
GACCTTGATAATCTTGATATTAGCGGCGGCGACCAAGAATCCACCAAAACCGATATCGACACCGATATTGATGACGCGCC
GATTGTTAGATTTGTGAACAAAATCCTGTTGGATGCCATAAAAAAAGGCGCCTCTGATATACACATGGAACCTTATGAAA
AAAACTTTCGTATTCGTTTCAGAGCGGATGGCATACTGTATCAGGTTGCCAGTCCGCCAACCAATATCGCCAGTCGAATA
ATATCCCGGGTTAAAGTGATGGCTAAAATGGATATTGCCGAACGTCGAGTACCGCAGGATGGGCGTATAAAAATGAACTT
GTCCAAGCACCTTGCGATTGATTTTCGTGTTAATAGCTGCCCCACATTATTCGGTGAAAAAGTGGTTTTGCGTATCTTGG
ACCCTACCAGTGCGCAGATTGGTATCGAAAAACTGGGCTTTGAGCCTGAACAAGAAAAGCTTTTTCTCCATGCCATTAAC
AAACCTTACGGTATGGTTCTGGTTACAGGACCTACGGGTAGCGGTAAAACGGTGACGCTGTACACCGGTTTAAACCTACT
CAACAGTATCGAACGCAATATTTGTACTGCTGAAGACCCCGTTGAAATTACTGTAGAAGGTATCAATCAGGTAAATATGA
ACGTTAAAGCCGGCTTGACGTTTGCTAGCGCCTTACGGGCTTTCTTGCGTCAAGATCCCGATGTTATTATGGTCGGTGAA
ATCAGGGATTTGGAAACCGCTGATATTGCCGTAAAAGCCGCGCAAACCGGTCACTTGGTATTATCAACTTTACATACCAA
TGATGCCCCGCAAACATTAAACCGTTTGATGCAAATGGGCATCGAGCCTTTCAACATAGTCTCTGCTGTTATTTTGATTA
TGGCTCAGCGTCTGGCGCGACGTCTTTGTGAGTACTGTAAAATCCCGGCTGATTTTCCCGAGCATATTCTTGTCTCTGCA
GGTTTTACAGAAGACGAGTTAAAGACACTTAAAATATATAGTTCTGTAGGTTGTGAGCACTGTACCAATGGTTATAAAGG
CCGGGTCGGTATTTATCAGGTAATGACCTTGAGTGAAAACATGCGCGCGCTGATATTGGAAGGCGGCAATGCCATGCAAT
TGGCAGAGCAGGCAAAAGCTGAAGGCATCAATGACCTTCGTGCCTCAGGCTTAAATAAAGTACGCATGGGTATCACCAGT
CTTGAAGAAATTGATCGGGTAACCAAGGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-144)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

53.043

100

0.535
  pilB Acinetobacter baylyi ADP1

53.147

100

0.533
  pilB Acinetobacter baumannii D1279779

52.622

100

0.528
  pilF Neisseria gonorrhoeae MS11

48.036

98.246

0.472
  pilB Vibrio parahaemolyticus RIMD 2210633

45.893

98.246

0.451
  pilB Vibrio cholerae strain A1552

49.706

89.649

0.446
  pilB Vibrio campbellii strain DS40M4

45.57

97.018

0.442
  pilB/pilB1 Synechocystis sp. PCC 6803

39.206

100

0.398
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.745

95.088

0.368