Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   R2APBS1_RS04440 Genome accession   NC_020541
Coordinates   1014176..1015447 (-) Length   423 a.a.
NCBI ID   WP_015447035.1    Uniprot ID   M4NBT7
Organism   Rhodanobacter denitrificans     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1009176..1020447
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R2APBS1_RS04405 (R2APBS1_0902) - 1009996..1010790 (+) 795 WP_015447030.1 thymidylate synthase -
  R2APBS1_RS04410 (R2APBS1_0903) - 1010917..1011405 (+) 489 WP_015447031.1 dihydrofolate reductase -
  R2APBS1_RS04415 (R2APBS1_0904) - 1011405..1011821 (+) 417 WP_027485879.1 NUDIX domain-containing protein -
  R2APBS1_RS04425 (R2APBS1_0905) coaE 1012119..1012733 (-) 615 WP_015447032.1 dephospho-CoA kinase -
  R2APBS1_RS04430 (R2APBS1_0906) pilD 1012736..1013593 (-) 858 WP_015447033.1 A24 family peptidase Machinery gene
  R2APBS1_RS04435 (R2APBS1_0907) - 1013608..1014027 (-) 420 WP_015447034.1 DUF559 domain-containing protein -
  R2APBS1_RS04440 (R2APBS1_0908) pilC 1014176..1015447 (-) 1272 WP_015447035.1 type II secretion system F family protein Machinery gene
  R2APBS1_RS04445 (R2APBS1_0909) pilB 1015590..1017314 (-) 1725 WP_015447036.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R2APBS1_RS04450 (R2APBS1_0910) - 1017947..1018189 (+) 243 WP_015447037.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
  R2APBS1_RS04455 (R2APBS1_0911) - 1018186..1018581 (+) 396 WP_015447038.1 type II toxin-antitoxin system VapC family toxin -
  R2APBS1_RS04460 (R2APBS1_0912) - 1018872..1019726 (+) 855 WP_008438588.1 glycosyltransferase family 2 protein -

Sequence

Protein


Download         Length: 423 a.a.        Molecular weight: 46090.32 Da        Isoelectric Point: 9.8580

>NTDB_id=57130 R2APBS1_RS04440 WP_015447035.1 1014176..1015447(-) (pilC) [Rhodanobacter denitrificans]
MATATATNINKARELGAQRAEVSKLTTYEWIALDKRGKRMKGDMPAKNASLVKAELRRQGMNPQTVRERAKPLFGATGST
VKPGDVAVFSRQIATMMASGVPMVQSFDIIADGQKNIRFKNILLDVKQNIEGGAALHEALARYPVQFDELYCNLVHAGET
SGVLDTVLDTVATYKERTEAIKKKIKKALFYPMMVLVVVFLVCLIMLLFVVPVFAKTFQDAGAQLPAPTQLLVTASEFMQ
SYWWVVIGVTVGGVAALIIAKKRSVKFAHFLDRMALKMPVMGNIVRNSAIARFARTLGVTFRAGVPLVEALDAVAGATGS
VVYSDAVKQMREDISVGHQLQLAMKQTGLFPNMVVQMTAIGEESGSLDNMLFKVAEFYEEEVSNAVDTLSTLLEPIIMVV
LGTLVGGMVIALYLPIFKLAGTF

Nucleotide


Download         Length: 1272 bp        

>NTDB_id=57130 R2APBS1_RS04440 WP_015447035.1 1014176..1015447(-) (pilC) [Rhodanobacter denitrificans]
ATGGCCACGGCTACCGCAACGAACATCAACAAGGCGCGCGAGCTCGGGGCCCAGCGCGCCGAAGTCAGCAAGCTGACCAC
CTACGAGTGGATTGCGCTGGACAAGCGCGGCAAGCGCATGAAAGGCGACATGCCGGCGAAGAACGCCTCGCTGGTCAAGG
CCGAACTGCGCCGCCAGGGCATGAACCCGCAGACCGTGCGCGAGCGTGCCAAGCCGCTGTTCGGCGCCACCGGCAGCACG
GTCAAGCCGGGCGACGTGGCCGTGTTCAGCCGCCAGATCGCCACCATGATGGCGTCGGGCGTGCCGATGGTGCAGTCCTT
CGACATCATCGCCGACGGCCAGAAGAACATCCGTTTCAAGAACATCCTGCTCGACGTGAAGCAGAACATCGAGGGCGGCG
CGGCGCTGCACGAGGCGCTGGCGCGCTACCCGGTGCAGTTCGACGAGCTGTATTGCAACCTGGTGCACGCCGGCGAGACC
TCCGGCGTGCTGGATACCGTGCTGGACACCGTGGCGACCTACAAGGAGCGCACGGAAGCGATCAAGAAGAAGATCAAGAA
GGCGCTGTTCTACCCGATGATGGTGCTGGTGGTGGTGTTCCTGGTCTGCCTGATCATGCTGTTGTTCGTGGTGCCGGTGT
TCGCCAAGACCTTCCAGGATGCCGGCGCGCAGCTGCCGGCGCCGACCCAGTTGCTGGTGACCGCGTCGGAGTTCATGCAG
AGCTACTGGTGGGTGGTGATCGGCGTGACCGTGGGCGGCGTCGCCGCGCTGATCATCGCCAAGAAGCGCTCGGTGAAGTT
CGCCCACTTCCTCGACCGCATGGCGCTGAAGATGCCGGTGATGGGCAATATCGTGCGCAACTCGGCGATCGCCCGCTTCG
CCCGCACCTTGGGCGTGACGTTCCGCGCCGGCGTGCCGCTGGTCGAGGCGCTGGACGCGGTGGCCGGCGCCACCGGCAGC
GTGGTCTACAGCGATGCCGTGAAGCAGATGCGCGAGGACATCTCGGTCGGCCACCAGCTGCAGCTGGCGATGAAGCAGAC
CGGGCTGTTCCCGAACATGGTGGTGCAGATGACCGCGATCGGCGAAGAATCCGGCTCGCTGGACAACATGCTGTTCAAGG
TTGCCGAGTTCTACGAGGAAGAAGTCAGCAACGCGGTCGACACCCTGTCCACGTTGCTCGAACCGATCATCATGGTGGTG
CTGGGTACCCTGGTCGGTGGCATGGTGATCGCGCTGTACCTGCCGATCTTCAAGCTCGCCGGCACGTTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(293-415)

Type II secretion system (T2SS), protein F (T2SSF)

(89-212)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M4NBT7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

54.177

93.381

0.506
  pilC Pseudomonas stutzeri DSM 10701

52.393

93.853

0.492
  pilC Acinetobacter baylyi ADP1

48.522

95.981

0.466
  pilC Acinetobacter baumannii D1279779

49.118

93.853

0.461
  pilG Neisseria gonorrhoeae MS11

41.406

90.78

0.376
  pilG Neisseria meningitidis 44/76-A

41.514

90.544

0.376
  pilC Vibrio campbellii strain DS40M4

38.346

94.326

0.362

Multiple sequence alignment