Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KJY40_RS03015 Genome accession   NZ_CP075567
Coordinates   672804..674882 (+) Length   692 a.a.
NCBI ID   WP_230734882.1    Uniprot ID   -
Organism   Pseudomonas fitomaticsae strain FIT81     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 667804..679882
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJY40_RS02995 (KJY40_02995) comM 670018..671082 (+) 1065 WP_007952389.1 pilus assembly protein PilM Machinery gene
  KJY40_RS03000 (KJY40_03000) - 671082..671648 (+) 567 WP_230734876.1 PilN domain-containing protein -
  KJY40_RS03005 (KJY40_03005) pilO 671645..672268 (+) 624 WP_230734878.1 type 4a pilus biogenesis protein PilO -
  KJY40_RS03010 (KJY40_03010) - 672265..672789 (+) 525 WP_230734880.1 pilus assembly protein PilP -
  KJY40_RS03015 (KJY40_03015) pilQ 672804..674882 (+) 2079 WP_230734882.1 type IV pilus secretin PilQ Machinery gene
  KJY40_RS03020 (KJY40_03020) aroK 674887..675405 (+) 519 WP_011332081.1 shikimate kinase AroK -
  KJY40_RS03025 (KJY40_03025) aroB 675558..676658 (+) 1101 WP_230734883.1 3-dehydroquinate synthase -
  KJY40_RS03030 (KJY40_03030) - 676669..678276 (+) 1608 WP_230734885.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 75083.24 Da        Isoelectric Point: 5.5724

>NTDB_id=569730 KJY40_RS03015 WP_230734882.1 672804..674882(+) (pilQ) [Pseudomonas fitomaticsae strain FIT81]
MNRIFSTLGFSLWMALLSPMVQAANLKALDVAALPGDRVELKLSFDGPPPQPKGYTTDSPARIALDLPGVASQLTSKTRD
LGSGNARTATVVEANNRTRLIISLTQLAPYDTRVEGNTVFVVVGQGSKAAAPRTVARAPRAAPAAAPVRTFTPTARAIRG
VDFQRGTAGEGNVVIDLSDPAIAPDIQEHDGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNAGVSGDRATITVEPSGT
FDYSTYQTDNKLTVSIRPLTVDDLQKRNADRNSYNGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNV
PWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEAK
VDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVSN
GSSTTIGTPGSTSTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=569730 KJY40_RS03015 WP_230734882.1 672804..674882(+) (pilQ) [Pseudomonas fitomaticsae strain FIT81]
ATGAACAGGATTTTTTCCACCCTCGGTTTTTCGCTATGGATGGCGCTGCTTTCACCGATGGTACAGGCAGCCAATCTGAA
GGCGCTGGACGTCGCGGCACTGCCGGGCGACCGGGTCGAGTTGAAGCTGTCGTTCGACGGCCCGCCACCACAGCCCAAGG
GCTACACCACTGATTCGCCGGCACGGATCGCCCTCGATCTGCCGGGTGTCGCCAGTCAGCTGACGAGCAAGACCCGTGAC
CTGGGCAGTGGCAATGCCCGCACGGCCACAGTGGTCGAGGCCAATAACCGGACGCGGCTGATCATCAGCCTGACGCAACT
GGCACCGTACGACACCCGGGTCGAAGGCAATACGGTGTTTGTGGTGGTCGGGCAGGGCAGCAAAGCCGCTGCCCCACGTA
CGGTCGCTCGTGCACCGCGCGCAGCACCTGCGGCAGCGCCGGTCCGCACGTTTACGCCGACCGCCAGAGCCATTCGCGGC
GTGGATTTCCAGCGTGGTACGGCGGGCGAGGGCAACGTGGTCATCGACTTGTCCGACCCGGCTATCGCCCCGGACATCCA
GGAGCACGACGGCAAGATCATCCTCAATTTTGCCCGTACCCAATTGCCTGAGCCACTGCGCGTGCGCCTCGACGTCAAGG
ATTTCGCCACCCCGGTGCAGTTCGTCAATGCCGGTGTCAGCGGTGATCGCGCGACGATCACCGTCGAGCCCAGCGGTACT
TTCGACTATTCCACTTACCAGACCGACAACAAGCTGACCGTCAGCATCCGGCCGTTGACCGTCGATGACCTGCAAAAGCG
CAACGCCGACCGCAACAGCTATAACGGCGAAAAGCTCTCTCTCAACTTCCAGGACATCGATGTGCGCTCGGTGCTGCAAC
TGATCGCCGACTTCACCAACCTCAATCTGGTGGCCAGCGATACGGTGCAGGGCGGCATTACCTTGCGCCTGCAGAACGTG
CCGTGGGATCAGGCGCTGGATCTGGTGCTCAAGACCAAAGGCCTGGACAAGCGCAAGGTCGGCAACGTGCTGCTGGTGGC
GCCGGCGGATGAAATCGCCGCCCGTGAACGGCAGGAACTGGAGTCGCAGAAACAGATTGCCGAGCTGGCGCCGCTGCGCC
GTGAGCTGTTGCAAGTGAACTACGCCAAGGCTGCGGACATCGCCAAGCTGTTCCAGTCGGTCACCAGTGCCGAGGCCAAG
GTCGACGAACGGGGTTCGATCACCGTCGATGAGCGCACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGACGA
GCTGCGGCGGATTGTGGCGCAGCTGGATATTCCGGTGCGTCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCG
ACTACGACAAAAGTCTGGGCGTGCGCTGGGGCGGTTCGATCCAGAACAAGGGCAACTGGAACACTTCCGGGGTCAGCAAC
GGTTCGTCGACCACCATTGGCACGCCGGGCAGCACCAGCACCAACTCGCCGTTCGTCGACATGGGCACGGTCAACAACAC
GTCCGGGATCGGTATCGCCTTCATCACCGACAACGTCTTGCTGGACCTTGAGCTGACCGCGATGGAGAAGACCGGTAACG
GCGAAATCGTCTCGCAGCCCAAGGTGGTCACCTCCGACAAGGAGACCGCGAAGATCCTCAAGGGCACCGAGATTCCGTAC
CAGGAAGCCAGCTCCAGCGGCGCTACGTCGGTGTCCTTCAAGGAGGCTTCGCTGTCGCTGGAAGTGACGCCGCAGATCAC
CCCGGACAACCGCATCATCATGGAGGTGAAGGTCACCAAGGACGAACCGGATTACCTGAACAAAGTGCAGGATGTACCGC
CGATCAAGAAAAACGAGGTCAATGCCAAGGTGCTGGTGAACGACGGCGAGACCATCGTGATCGGGGGCGTTTTCTCAAAT
ACTCAAAGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(302-348)

Bacterial type II/III secretion system short domain (Secretin_N)

(376-441)

AMIN domain (AMIN)

(37-121)

Bacterial type II and III secretion system protein (Secretin)

(527-683)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.608

100

0.74
  comQ Acinetobacter baylyi ADP1

40.22

100

0.422
  pilQ Acinetobacter baumannii strain A118

37.65

100

0.408
  pilQ Acinetobacter baumannii D1279779

37.65

100

0.408
  pilQ Legionella pneumophila strain Lp02

37.63

97.543

0.367
  pilQ Legionella pneumophila strain ERS1305867

37.593

97.254

0.366