Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   KJY40_RS02995 Genome accession   NZ_CP075567
Coordinates   670018..671082 (+) Length   354 a.a.
NCBI ID   WP_007952389.1    Uniprot ID   J2PGW6
Organism   Pseudomonas fitomaticsae strain FIT81     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 665018..676082
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJY40_RS02985 (KJY40_02985) - 665844..667112 (+) 1269 WP_007952395.1 malic enzyme-like NAD(P)-binding protein -
  KJY40_RS02990 (KJY40_02990) - 667353..669797 (-) 2445 WP_407681995.1 penicillin-binding protein 1A -
  KJY40_RS02995 (KJY40_02995) comM 670018..671082 (+) 1065 WP_007952389.1 pilus assembly protein PilM Machinery gene
  KJY40_RS03000 (KJY40_03000) - 671082..671648 (+) 567 WP_230734876.1 PilN domain-containing protein -
  KJY40_RS03005 (KJY40_03005) pilO 671645..672268 (+) 624 WP_230734878.1 type 4a pilus biogenesis protein PilO -
  KJY40_RS03010 (KJY40_03010) - 672265..672789 (+) 525 WP_230734880.1 pilus assembly protein PilP -
  KJY40_RS03015 (KJY40_03015) pilQ 672804..674882 (+) 2079 WP_230734882.1 type IV pilus secretin PilQ Machinery gene
  KJY40_RS03020 (KJY40_03020) aroK 674887..675405 (+) 519 WP_011332081.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38097.60 Da        Isoelectric Point: 4.6857

>NTDB_id=569729 KJY40_RS02995 WP_007952389.1 670018..671082(+) (comM) [Pseudomonas fitomaticsae strain FIT81]
MLGLFNKKANTLLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPASAVVEKNIAELEGVGQALSRVLVKARTGLKTVAV
AVAGSAVITKVIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQDRLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTL
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEERLNTPTQVANP
FSDMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=569729 KJY40_RS02995 WP_007952389.1 670018..671082(+) (comM) [Pseudomonas fitomaticsae strain FIT81]
GTGCTAGGACTCTTCAATAAAAAGGCCAATACGTTACTGGGGATCGACATCAGCTCCACTTCGGTGAAGCTGCTGGAGCT
GAGCCGTCAGGGTGAGCGCTACCGGGTCGAGGCGTATGCGGTGGAGCCGTTGCCGGCGAGCGCCGTGGTCGAAAAGAACA
TCGCCGAGCTCGAGGGCGTCGGCCAGGCCCTGTCCCGGGTGCTGGTCAAGGCGCGCACCGGACTGAAAACCGTGGCGGTG
GCGGTGGCCGGCTCTGCGGTGATCACCAAGGTCATCGAGATGGACGCCGGACTGTCCGACGACGAACTGGAAAACCAGCT
CAAGATCGAGGCCGATCAATACATTCCCTATCCGCTGGACGAGGTCGCCATCGACTTCGAAGTCCAGGGCGTTTCGCCGC
GCAACCCCGAGCGGGTCAACGTGCTGCTGGCCGCCTGTCGCAAGGAAAACGTCGAAGTCCGCGAAGCGGCGCTCGCTCTC
GCCGGGCTGACCGCGCGGGTGGTGGATGTCGAAGCCTATGCGCTGGAGCGCTCGTTTGGTCTGCTGGCCACGCAACTGGC
GGCGTCCCAGGATCGGCTGACGGTGGCCGTGGTCGACATCGGCGCCACCATGACCACCCTCAGCGTCCTGCACAACGGCC
GGATCATCTACACCCGCGAACAACTGTTCGGCGGCCGCCAGCTGACCGAGGAAATCCAGCGCCGTTATGGCTTGACCCTT
GAGCAGGCGGGGCTCGCCAAGAAGCAGGGCGGCCTGCCGGACGATTACGTCAGCGAGGTGCTGCAACCGTTTCGCGAGGC
ACTGGTGCAGCAGGTGTCGCGCTCCCTGCAGTTCTTTTTCGCCTCCGGCCAGTACAACGCCGTCGACCACATTCTGCTGG
CCGGCGGCACGGCGTCGGTGCCCGGCCTCGACCGGCTGATCGAAGAGCGCCTGAACACCCCGACTCAGGTCGCCAACCCG
TTTTCCGACATGGCCTTGAGCAGCAAGGTCAACGCCGGGGCCCTGGCGAGCGATGCGCCGGCCCTGATGATTGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB J2PGW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  pilM Acinetobacter baumannii D1279779

55.932

100

0.559
  comM Acinetobacter baylyi ADP1

53.955

100

0.54
  pilM Legionella pneumophila strain ERS1305867

49.577

100

0.497