Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KI608_RS03475 Genome accession   NZ_CP075192
Coordinates   734760..736475 (-) Length   571 a.a.
NCBI ID   WP_226450636.1    Uniprot ID   -
Organism   Ferribacterium limneticum strain 132     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 729760..741475
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI608_RS03450 (KI608_03455) aspS 729911..731710 (-) 1800 WP_226450632.1 aspartate--tRNA ligase -
  KI608_RS03455 (KI608_03460) - 731728..732342 (-) 615 WP_226451833.1 DUF502 domain-containing protein -
  KI608_RS03460 (KI608_03465) - 732342..732593 (-) 252 WP_226450633.1 zinc ribbon domain-containing protein -
  KI608_RS03465 (KI608_03470) pilD 732664..733518 (-) 855 WP_226450634.1 A24 family peptidase Machinery gene
  KI608_RS03470 (KI608_03475) pilC 733518..734744 (-) 1227 WP_226450635.1 type II secretion system F family protein Machinery gene
  KI608_RS03475 (KI608_03480) pilB 734760..736475 (-) 1716 WP_226450636.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KI608_RS03480 (KI608_03485) - 736555..737739 (-) 1185 WP_404826784.1 HlyC/CorC family transporter -
  KI608_RS03485 (KI608_03490) - 737830..738669 (-) 840 WP_226450638.1 inner membrane protein YpjD -
  KI608_RS03490 (KI608_03495) ffh 738699..740066 (+) 1368 WP_226450639.1 signal recognition particle protein -
  KI608_RS03495 (KI608_03500) - 740067..740678 (-) 612 WP_226450640.1 lytic transglycosylase domain-containing protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 61900.30 Da        Isoelectric Point: 5.9489

>NTDB_id=568597 KI608_RS03475 WP_226450636.1 734760..736475(-) (pilB) [Ferribacterium limneticum strain 132]
MAATPQNPPLSGLARALVQAGHLKETEAEQLLAQAHTSKTSLIEQIITSQKSSAIDIARFVADTFGYPLLDLNAFDEAHI
PSDAIDRKLIATHKVIPLNKRGNRLSVAIADPTNLRALDEIRFQTGLAVDPIVVEHPKLAPLVTKYAETAAEALKNFTSE
DLNLDFLDEEAASKTDEAAGQEIDDAPVVKFIQKMLLDAINDGASDIHFEPYEKFYRIRFRVDGILREVATPPLAIKEKI
ASRIKVISRLNIAEKRVPQDGRMKLVLSKTRAIDFRVSTLPTLQGEKIVMRILDPSSATLGIEALGYEPEQKAALMDAIG
RPYGMVLVTGPTGSGKTVSLYTCLNILNKDGINISTAEDPAEINLSGINQVNVDDRAGLTFPVALKAFLRQDPDIIMVGE
IRDLETAEISIKAAQTGHLVLSTLHTNDAPQTLTRLMNMGVPMFNIASSVLLITAQRLARRLCNCKKPMTVPNQALLDAG
YSEADLDGSWTLFGPGGCERCKGTGYKGRVGIYQVMPISEAMQRMIMSGASALDLGAQAKAEGVKNLRESGLLKVKQGVT
SLDEVLSTTNA

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=568597 KI608_RS03475 WP_226450636.1 734760..736475(-) (pilB) [Ferribacterium limneticum strain 132]
ATGGCAGCAACCCCTCAAAATCCTCCTCTCAGCGGCTTGGCGCGTGCGCTTGTCCAGGCAGGGCATCTCAAGGAGACAGA
GGCGGAACAATTGCTGGCCCAGGCCCACACATCCAAGACTTCGCTGATCGAACAGATCATCACAAGCCAGAAATCCAGTG
CAATCGACATTGCACGCTTTGTCGCAGATACGTTTGGCTACCCGTTACTTGACCTCAACGCCTTCGACGAAGCCCACATT
CCGTCGGACGCCATCGACCGTAAGCTGATCGCCACACACAAGGTCATCCCGCTTAACAAGCGCGGCAATCGTTTATCTGT
CGCAATCGCTGATCCAACCAATCTACGCGCCCTTGACGAAATTCGCTTCCAGACCGGGCTGGCCGTAGACCCTATCGTCG
TCGAGCACCCAAAACTGGCGCCTCTGGTCACCAAATACGCCGAAACTGCAGCCGAGGCACTAAAGAACTTCACCAGCGAG
GATCTCAACCTCGACTTTCTGGACGAGGAAGCTGCCAGCAAGACTGACGAAGCTGCAGGACAGGAAATCGACGATGCGCC
AGTCGTCAAATTCATCCAGAAAATGTTGCTCGATGCCATCAACGATGGGGCATCGGACATCCATTTCGAACCATACGAGA
AGTTTTACCGTATTCGCTTCCGCGTCGATGGCATCCTCCGCGAAGTGGCAACGCCACCGCTGGCCATCAAGGAAAAAATC
GCCTCCCGTATCAAGGTAATTTCCCGACTCAATATCGCCGAGAAACGCGTCCCGCAGGATGGTCGGATGAAATTGGTGCT
TTCAAAGACCCGCGCCATCGATTTCCGGGTCAGCACACTACCAACGCTTCAGGGCGAGAAAATCGTCATGCGTATTCTCG
ACCCAAGTTCTGCCACCTTGGGCATTGAGGCGCTAGGCTACGAGCCCGAGCAAAAAGCAGCGCTGATGGATGCCATCGGC
CGGCCTTACGGCATGGTGCTGGTCACCGGGCCAACCGGCTCAGGCAAGACAGTCTCGCTCTACACCTGCCTCAACATCCT
GAATAAGGATGGCATCAACATTTCGACCGCGGAAGACCCTGCCGAAATCAATCTCTCCGGAATCAATCAGGTCAACGTGG
ATGATCGTGCCGGCCTGACCTTCCCTGTGGCGCTGAAGGCCTTCCTGCGCCAGGATCCGGACATCATCATGGTCGGGGAA
ATCCGCGACTTGGAAACTGCCGAGATTTCCATCAAGGCGGCCCAAACGGGCCACTTGGTGCTCTCGACGCTGCACACCAA
CGACGCCCCGCAAACACTGACTCGCCTCATGAATATGGGTGTCCCCATGTTCAATATCGCCTCCAGCGTGCTGCTAATCA
CCGCCCAGCGCTTGGCGCGACGGCTATGTAACTGCAAGAAACCGATGACTGTACCCAACCAGGCGCTTCTGGATGCAGGC
TACTCGGAAGCTGATCTCGACGGCTCGTGGACACTGTTCGGGCCAGGGGGCTGCGAACGCTGCAAAGGAACGGGCTACAA
GGGGCGCGTTGGCATCTATCAGGTCATGCCTATTTCCGAAGCCATGCAGCGCATGATCATGAGCGGCGCCTCGGCTCTGG
ATCTGGGAGCCCAGGCAAAAGCAGAAGGCGTAAAGAATCTCCGCGAATCCGGACTATTAAAAGTCAAACAGGGTGTGACC
TCGCTTGACGAGGTACTGAGCACCACCAACGCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.594

100

0.557
  pilB Acinetobacter baumannii D1279779

54.545

100

0.546
  pilB Legionella pneumophila strain ERS1305867

53.497

100

0.536
  pilF Neisseria gonorrhoeae MS11

51.957

98.424

0.511
  pilB Vibrio cholerae strain A1552

49.558

98.949

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

48.582

98.774

0.48
  pilB Vibrio campbellii strain DS40M4

47.965

98.949

0.475
  pilF Thermus thermophilus HB27

39.543

99.65

0.394
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.432

98.599

0.389