Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KI612_RS03595 Genome accession   NZ_CP075188
Coordinates   738474..740189 (-) Length   571 a.a.
NCBI ID   WP_226442472.1    Uniprot ID   -
Organism   Quatrionicoccus australiensis strain D9     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 733474..745189
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI612_RS03570 (KI612_03575) aspS 733621..735420 (-) 1800 WP_226442468.1 aspartate--tRNA ligase -
  KI612_RS03575 (KI612_03580) - 735442..736056 (-) 615 WP_226444129.1 DUF502 domain-containing protein -
  KI612_RS03580 (KI612_03585) - 736056..736310 (-) 255 WP_226442469.1 zinc ribbon domain-containing protein -
  KI612_RS03585 (KI612_03590) pilD 736381..737232 (-) 852 WP_226442470.1 A24 family peptidase Machinery gene
  KI612_RS03590 (KI612_03595) pilC 737232..738458 (-) 1227 WP_226442471.1 type II secretion system F family protein Machinery gene
  KI612_RS03595 (KI612_03600) pilB 738474..740189 (-) 1716 WP_226442472.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KI612_RS03600 (KI612_03605) - 740277..741539 (-) 1263 WP_226442473.1 HlyC/CorC family transporter -
  KI612_RS03605 (KI612_03610) - 741545..742384 (-) 840 WP_226442474.1 inner membrane protein YpjD -
  KI612_RS03610 (KI612_03615) ffh 742414..743772 (+) 1359 WP_226442475.1 signal recognition particle protein -
  KI612_RS03615 (KI612_03620) - 743773..744360 (-) 588 WP_226444131.1 lytic transglycosylase domain-containing protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 61914.30 Da        Isoelectric Point: 5.4465

>NTDB_id=568503 KI612_RS03595 WP_226442472.1 738474..740189(-) (pilB) [Quatrionicoccus australiensis strain D9]
MAATPQTTPLSGLARALVQAGQLKEAEAEQLISQASSAKIPLIEQIIASQKASALDIARFVADTFGYPLLDLNAFDEAHI
PATAIDRKLMATHKVIPLNKRGNRLSVAISDPTNLRALDEIRFQTGLAVDPIVVEHPKLIPLVSKFSESAEDSLKNLGGD
DINLDFLDEESTSKADEAAGQEIDDAPVVKFIQKMLLDAINDGASDIHFEPYEKYYRIRFRVDGILREVATPPLAIKEKI
ASRIKVISRLNIAEKRVPQDGRMKLVLSKTRAIDFRVSTLPTLQGEKIVLRILDPTSATLGIEALGYEPEQKEALLDAVN
RPYGMVLVTGPTGSGKTVSLYTCLNILNKDGVNISTAEDPAEINLPGVNQVNVDDRAGLTFPVALKAFLRQDPDIIMVGE
IRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTLTRLMNMGVPTFNIASSIQLITAQRLARRLCTCKKPMTVPNQALLDAG
FAESDLDGSWTLYGPVGCERCKGSGYKGRVGIYQVMPVSEAMQRLIMSGANSLDLGKQAAAEGVKNLRESGLLKVKQGMT
SLDEVLSTTNI

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=568503 KI612_RS03595 WP_226442472.1 738474..740189(-) (pilB) [Quatrionicoccus australiensis strain D9]
ATGGCAGCGACACCTCAAACTACTCCTCTTAGCGGCCTGGCACGAGCACTGGTACAAGCCGGGCAACTCAAGGAGGCAGA
GGCCGAACAACTGATCTCGCAAGCGAGTTCAGCCAAGATCCCACTGATCGAACAGATCATCGCCAGCCAGAAGGCTTCGG
CCCTTGATATCGCACGCTTTGTCGCCGATACCTTTGGTTATCCGCTTCTGGATCTCAATGCCTTCGATGAAGCCCACATC
CCGGCAACGGCCATCGACCGCAAGCTCATGGCCACCCACAAGGTCATTCCGCTCAACAAGCGCGGCAACCGGCTGTCGGT
TGCCATCTCCGACCCGACCAATCTGCGGGCACTGGATGAAATCCGCTTCCAGACAGGTCTCGCGGTCGACCCGATTGTTG
TCGAACATCCCAAGCTGATCCCGCTGGTCAGCAAGTTCTCCGAATCCGCCGAAGACAGCCTGAAGAATCTTGGCGGCGAT
GATATCAACCTCGACTTCCTCGACGAAGAATCCACTTCCAAGGCAGACGAAGCGGCAGGACAGGAAATCGACGATGCCCC
GGTCGTCAAGTTCATCCAGAAGATGCTGCTCGACGCCATCAACGATGGCGCATCCGACATCCACTTCGAGCCGTATGAAA
AATATTACCGCATCCGCTTCCGGGTCGACGGCATCCTGCGCGAAGTTGCAACGCCGCCGCTCGCCATCAAGGAAAAGATC
GCCTCCCGCATCAAGGTCATTTCCCGGCTGAATATCGCCGAAAAGCGCGTACCGCAGGATGGCCGGATGAAACTGGTGCT
TTCCAAGACCCGCGCCATCGACTTCCGGGTCAGCACCCTGCCGACACTGCAAGGCGAAAAGATCGTTCTGCGTATTCTCG
ACCCGACCTCGGCGACGCTCGGCATCGAGGCCCTGGGTTACGAGCCGGAACAGAAGGAAGCCCTGCTTGATGCGGTCAAT
CGTCCCTACGGCATGGTTCTCGTCACTGGCCCGACCGGCTCCGGCAAAACGGTATCGCTCTATACCTGCCTCAACATCCT
GAACAAGGATGGCGTCAATATCTCGACCGCAGAAGACCCTGCGGAAATCAACCTGCCCGGCGTCAATCAGGTCAACGTCG
ACGACCGCGCCGGCCTGACCTTCCCGGTAGCACTCAAGGCCTTCCTGCGGCAGGATCCGGACATCATCATGGTCGGTGAA
ATCCGCGACCTCGAAACGGCCGAAATCGCCATCAAGGCAGCCCAGACCGGCCACATGGTACTTTCCACGCTACACACGAA
TGACGCACCACAAACCCTGACGCGACTGATGAACATGGGGGTCCCCACCTTCAATATCGCATCGAGTATCCAGCTGATCA
CGGCACAACGACTCGCTCGCCGGCTATGCACCTGCAAAAAACCGATGACGGTCCCCAATCAGGCCCTGCTTGATGCCGGC
TTTGCCGAGTCCGATCTCGATGGCTCGTGGACGCTTTACGGCCCGGTCGGTTGCGAACGCTGCAAAGGCAGTGGCTACAA
GGGGCGGGTCGGCATCTATCAGGTCATGCCGGTCTCTGAGGCCATGCAACGCCTGATCATGAGCGGCGCCAACTCCCTCG
ACCTGGGGAAACAGGCAGCGGCCGAAGGCGTCAAGAATCTGCGCGAATCCGGTTTGCTCAAGGTCAAGCAAGGCATGACT
TCGCTTGATGAAGTGCTGAGCACCACCAACATATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.282

99.475

0.55
  pilB Legionella pneumophila strain ERS1305867

53.993

100

0.545
  pilB Acinetobacter baumannii D1279779

53.846

100

0.539
  pilF Neisseria gonorrhoeae MS11

51.389

100

0.518
  pilB Vibrio cholerae strain A1552

48.577

98.424

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

48.404

98.774

0.478
  pilB Vibrio campbellii strain DS40M4

48.214

98.074

0.473
  pilF Thermus thermophilus HB27

38.354

100

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.245

92.82

0.364