Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KI611_RS18045 Genome accession   NZ_CP075185
Coordinates   3774148..3775863 (+) Length   571 a.a.
NCBI ID   WP_226417032.1    Uniprot ID   -
Organism   Dechloromonas denitrificans strain 110     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3769148..3780863
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI611_RS18025 (KI611_17980) - 3769957..3770568 (+) 612 WP_226417028.1 lytic transglycosylase domain-containing protein -
  KI611_RS18030 (KI611_17985) ffh 3770569..3771927 (-) 1359 WP_226417029.1 signal recognition particle protein -
  KI611_RS18035 (KI611_17990) - 3771957..3772796 (+) 840 WP_226417030.1 inner membrane protein YpjD -
  KI611_RS18040 (KI611_17995) - 3772800..3774071 (+) 1272 WP_226417031.1 HlyC/CorC family transporter -
  KI611_RS18045 (KI611_18000) pilB 3774148..3775863 (+) 1716 WP_226417032.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KI611_RS18050 (KI611_18005) pilC 3775878..3777104 (+) 1227 WP_226417033.1 type II secretion system F family protein Machinery gene
  KI611_RS18055 (KI611_18010) pilD 3777104..3777952 (+) 849 WP_226417034.1 prepilin peptidase Machinery gene
  KI611_RS18060 (KI611_18015) - 3778023..3778286 (+) 264 WP_226417035.1 FmdB family zinc ribbon protein -
  KI611_RS18065 (KI611_18020) - 3778286..3778900 (+) 615 WP_226419944.1 DUF502 domain-containing protein -
  KI611_RS18070 (KI611_18025) aspS 3778918..3780720 (+) 1803 WP_226417036.1 aspartate--tRNA ligase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 61854.23 Da        Isoelectric Point: 6.0483

>NTDB_id=568440 KI611_RS18045 WP_226417032.1 3774148..3775863(+) (pilB) [Dechloromonas denitrificans strain 110]
MAATPQNPPLSGLARALVQAGQLKEAEAEQLLSQANSAKTSLIEQIIASQKASALDIARFVSDTFGYPLLDLAAFDEAHI
PANAIDRKLISTHKVIPLNKRGNRLSVAIADPTNLRALDEIRFQTGLAVDPIVVEHPKLTPLVAKYAESAADTLKNLGAD
DINLDFLDEESVAKADEAAGQDIDDAPVVKFIQKMLLDAINDGASDIHFEPYEKFYRIRFRVDGILREIATPPLAIKEKV
ASRIKVISRLNIAEKRVPQDGRMRLVLSKTRAIDFRVSTLPTLQGEKIVLRILDPTSATLGIEALGYEPEQKVALLDAVN
RPYGMVLVTGPTGSGKTVSLYTCLNILNKDGVNISTAEDPAEINLPGVNQVNVDDRAGLTFPVALKAFLRQDPDIIMVGE
IRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTLTRLMNMGVPTFNIASSIQLITAQRLARRLCNCKKPMTVPNQALLDAG
YKEADLDGSWTLYGPGGCDRCKGSGFKGRVGIYQVMPISEAMQRLIMSGANSLDLARQAKAEGVKDLRESGLLKVKQGVT
SLDEVLSTTNV

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=568440 KI611_RS18045 WP_226417032.1 3774148..3775863(+) (pilB) [Dechloromonas denitrificans strain 110]
ATGGCAGCAACCCCGCAAAACCCTCCTCTTAGCGGCCTGGCGCGTGCCCTTGTCCAGGCCGGGCAACTCAAGGAGGCAGA
GGCCGAACAGTTGCTGTCCCAAGCCAACAGCGCCAAAACATCGCTGATCGAGCAGATCATCGCCAGCCAGAAAGCATCGG
CGCTTGATATTGCACGTTTTGTCTCTGACACCTTCGGTTATCCGCTGCTCGATCTCGCCGCTTTCGATGAAGCGCACATT
CCGGCAAATGCAATCGACCGCAAGCTGATTTCAACGCACAAGGTCATTCCACTCAACAAACGCGGCAACCGGCTGTCGGT
GGCCATTGCCGACCCGACCAATCTGCGGGCGCTGGACGAGATCCGCTTCCAGACCGGCCTTGCCGTCGACCCAATTGTCG
TCGAGCATCCGAAACTGACCCCGCTGGTTGCCAAGTACGCGGAATCAGCTGCCGACACGCTGAAGAATCTGGGTGCCGAC
GACATCAATCTGGACTTCCTTGACGAAGAGTCAGTGGCAAAGGCAGATGAAGCTGCCGGCCAGGACATCGATGATGCGCC
AGTAGTCAAATTCATTCAGAAAATGCTCCTGGATGCGATCAACGATGGCGCATCGGACATCCATTTCGAACCCTACGAAA
AGTTTTACCGTATCCGCTTCCGGGTTGATGGCATCTTGCGTGAAATCGCCACACCTCCGCTGGCAATCAAGGAAAAAGTT
GCCTCCCGGATCAAGGTCATTTCCCGCCTGAATATTGCCGAGAAGCGCGTCCCCCAGGACGGTCGGATGCGACTGGTCTT
ATCCAAGACACGGGCGATCGATTTCCGGGTCAGCACACTGCCCACGCTGCAGGGCGAGAAAATTGTGCTGCGTATTCTTG
ACCCGACTTCGGCGACCTTGGGTATCGAAGCCCTCGGCTACGAGCCGGAACAGAAAGTTGCCCTGCTCGATGCCGTCAAC
CGTCCATACGGCATGGTTCTGGTAACCGGACCGACCGGCTCCGGCAAGACCGTTTCGCTTTACACCTGCCTGAATATCCT
GAACAAGGACGGGGTTAACATTTCGACCGCCGAAGACCCGGCCGAAATCAATCTGCCTGGCGTCAATCAGGTCAACGTCG
ACGACCGCGCCGGTCTAACCTTCCCCGTCGCACTCAAGGCATTCCTGCGGCAGGATCCCGACATCATCATGGTCGGCGAA
ATCCGCGACCTCGAAACGGCCGAAATCGCCATCAAGGCCGCCCAGACCGGCCACATGGTACTGTCCACCCTACACACCAA
CGACGCCCCGCAGACGCTGACCCGTTTGATGAACATGGGGGTTCCGACCTTCAACATTGCTTCCAGCATCCAGTTGATCA
CGGCGCAGCGGCTGGCCCGGCGCCTATGCAATTGCAAAAAGCCAATGACCGTACCGAACCAGGCCCTGCTTGACGCTGGT
TACAAGGAGGCTGACCTAGATGGCTCCTGGACCCTCTATGGTCCCGGCGGCTGCGATCGGTGCAAGGGCAGCGGCTTCAA
GGGACGGGTCGGCATCTATCAGGTAATGCCGATCTCCGAAGCCATGCAGCGCTTGATCATGAGCGGCGCCAATTCCCTCG
ACCTCGCCCGCCAAGCCAAGGCAGAAGGTGTCAAGGATTTGCGCGAATCAGGATTGCTTAAAGTAAAACAGGGCGTGACC
TCGCTTGACGAGGTATTGAGCACGACCAACGTATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-150)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.72

100

0.548
  pilB Acinetobacter baumannii D1279779

54.007

100

0.543
  pilB Legionella pneumophila strain ERS1305867

52.668

100

0.536
  pilF Neisseria gonorrhoeae MS11

50.53

99.124

0.501
  pilB Vibrio cholerae strain A1552

48.227

98.774

0.476
  pilB Vibrio parahaemolyticus RIMD 2210633

48.043

98.424

0.473
  pilB Vibrio campbellii strain DS40M4

47.687

98.424

0.469
  pilF Thermus thermophilus HB27

38.095

99.299

0.378
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.567

97.898

0.368