Detailed information NTG0056787

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: LPE509_RS10735
• Genome accession: NC_020521
• Coordinates: 2433151..2434215 (-)
• Length: 354 a.a.
• NCBI ID: WP_010946662.1
• Uniprot ID: Q5ZX06
• Organism: Legionella pneumophila subsp. pneumophila LPE509
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2428151..2439215

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LPE509_RS10710 (LPE509_02278)	aroK	2428329..2428856 (-)	528	WP_010946667.1	shikimate kinase AroK	-
LPE509_RS10715 (LPE509_02279)	pilQ	2429302..2431398 (-)	2097	WP_010946666.1	type IV pilus secretin PilQ	Machinery gene
LPE509_RS10720 (LPE509_02280)	pilP	2431402..2431989 (-)	588	WP_010946665.1	pilus assembly protein PilP	Machinery gene
LPE509_RS10725 (LPE509_02281)	pilO	2431986..2432585 (-)	600	WP_014841277.1	type 4a pilus biogenesis protein PilO	Machinery gene
LPE509_RS10730 (LPE509_02282)	pilN	2432599..2433147 (-)	549	WP_010946663.1	PilN domain-containing protein	Machinery gene
LPE509_RS10735 (LPE509_02283)	pilM	2433151..2434215 (-)	1065	WP_010946662.1	type IV pilus assembly protein PilM	Machinery gene
LPE509_RS10740 (LPE509_02284)	ravI	2434632..2435642 (+)	1011	WP_010946661.1	Dot/Icm T4SS effector RavI	-
LPE509_RS10745 (LPE509_02285)	-	2435794..2438178 (+)	2385	WP_010946660.1	penicillin-binding protein 1A	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38732.71 Da
• Isoelectric Point: 5.0367

>NTDB_id=56787 LPE509_RS10735 WP_010946662.1 2433151..2434215(-) (pilM) [Legionella pneumophila subsp. pneumophila LPE509]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNIIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQSGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=56787 LPE509_RS10735 WP_010946662.1 2433151..2434215(-) (pilM) [Legionella pneumophila subsp. pneumophila LPE509]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATCCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGCGAAGAAATACCTGCCTCTGCCTTAGAAGGAAATATAA
TTAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAAATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTGGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAGTCTTATGCAGTAGAGCGTTCAGTACAACAAATCGCGCAAGAATTACC
AGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGGCAATCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCGCCTACACTGATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: Q5ZX06

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	99.435	100	0.994
comM	Acinetobacter nosocomialis M2	42.817	100	0.429
pilM	Acinetobacter baumannii D1279779	42.535	100	0.427
comM	Acinetobacter baylyi ADP1	41.457	100	0.418