Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AZKH_RS17690 Genome accession   NC_020516
Coordinates   3956341..3958059 (+) Length   572 a.a.
NCBI ID   WP_015437158.1    Uniprot ID   H0Q417
Organism   Azoarcus sp. KH32C     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3951341..3963059
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AZKH_RS17665 (AZKH_3566) - 3951678..3952469 (-) 792 WP_015437153.1 M48 family metallopeptidase -
  AZKH_RS17670 (AZKH_3567) - 3952451..3953203 (-) 753 WP_015437154.1 molybdopterin-binding protein -
  AZKH_RS17675 (AZKH_3568) - 3953213..3953974 (-) 762 WP_015437155.1 EI24 domain-containing protein -
  AZKH_RS17680 (AZKH_3569) ffh 3953990..3955348 (-) 1359 WP_015437156.1 signal recognition particle protein -
  AZKH_RS17685 (AZKH_3570) - 3955391..3956218 (+) 828 WP_015437157.1 inner membrane protein YpjD -
  AZKH_RS17690 (AZKH_3571) pilB 3956341..3958059 (+) 1719 WP_015437158.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AZKH_RS17695 (AZKH_3572) pilC 3958077..3959312 (+) 1236 WP_015437159.1 type II secretion system F family protein Machinery gene
  AZKH_RS17700 (AZKH_3574) pilD 3959367..3960218 (+) 852 WP_015437161.1 A24 family peptidase Machinery gene
  AZKH_RS17705 (AZKH_3575) - 3960347..3960610 (+) 264 WP_015437162.1 FmdB family zinc ribbon protein -
  AZKH_RS17710 (AZKH_3576) - 3960626..3961249 (+) 624 WP_041656347.1 DUF502 domain-containing protein -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62205.42 Da        Isoelectric Point: 5.3303

>NTDB_id=56718 AZKH_RS17690 WP_015437158.1 3956341..3958059(+) (pilB) [Azoarcus sp. KH32C]
MAANPQTALSGLARALVQHGRLSEADAATCTTSAPAVPNGFILELAQRKLITTKEVAWFAAETFGYPLLDIAAVDRSFFP
KDAIDRKLISKHQIIALGKRQNRLTVAAADPSNMRALDEIRFQTGMQVDLVIVEADRLARLAEALSESAADTLKELTGED
FDMDLLQGQEPAAETASDEGSAEVDDAPVVKFIQKVLIDAINEGASDIHFEPYEKYYRIRVRTDGILREIAQPPLVLKEK
IAARIKVISRLDISEKRVPQDGRMKLVLSKSKSIDFRVSTLPTLHGEKIVMRILDANSAMLGIDALGYEPDQKKVLMDAI
ERPYGMVLVTGPTGSGKTVSLYTCLNILNKPGVNISTAEDPAEINLPGINQVNVNEKAGLTFAASLRSFLRQDPDVIMVG
EIRDLETAEISVKAAQTGHLVLSTLHTNDAPTTLERLKNMGVAPFNIASSVIMITAQRLARRLCTCKQPVDIPIEALLQA
GFSGDELDGSWRPYGPVGCERCKGSGYKGRLGIYQVMPISDEIAHIIMTNGNSIDIAAQAQREGVRDLRQSGLLKVKQGA
TSLEEVLATTNE

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=56718 AZKH_RS17690 WP_015437158.1 3956341..3958059(+) (pilB) [Azoarcus sp. KH32C]
ATGGCAGCAAACCCTCAGACCGCACTGAGCGGCCTTGCACGAGCATTGGTTCAACACGGCCGCCTGTCGGAGGCGGATGC
GGCGACGTGCACGACCAGCGCACCGGCGGTCCCCAACGGGTTCATTCTGGAGCTCGCGCAGCGCAAACTGATCACCACCA
AGGAAGTCGCCTGGTTCGCCGCCGAAACCTTCGGCTACCCGCTACTTGATATCGCGGCTGTCGACCGCTCTTTCTTCCCG
AAGGATGCCATCGATCGCAAGCTGATCAGCAAACATCAGATCATTGCGCTCGGCAAGCGTCAGAACCGCCTCACCGTTGC
CGCCGCCGATCCGTCGAACATGCGGGCTCTCGACGAGATCCGGTTCCAAACGGGCATGCAAGTGGATCTGGTCATCGTCG
AGGCCGACCGGCTCGCCCGCCTCGCCGAAGCGTTGTCCGAATCTGCCGCCGACACGCTGAAAGAGCTGACCGGCGAAGAT
TTCGACATGGACCTCCTGCAGGGGCAGGAGCCTGCGGCCGAAACGGCATCTGACGAAGGCTCGGCGGAAGTTGACGATGC
GCCGGTCGTCAAATTCATTCAGAAAGTCCTGATCGATGCGATTAACGAAGGCGCATCCGACATCCACTTCGAGCCGTACG
AGAAGTACTACCGCATCCGGGTTCGGACCGACGGCATCCTGCGCGAGATCGCGCAACCGCCGCTGGTGCTCAAGGAAAAG
ATTGCCGCGCGGATCAAGGTGATATCGCGGCTCGATATTTCGGAAAAGCGCGTCCCGCAAGACGGCCGGATGAAGCTGGT
GCTGTCCAAGAGCAAGTCGATCGACTTTCGCGTTTCCACCTTGCCGACACTTCACGGCGAAAAGATCGTGATGCGTATTC
TGGACGCCAACTCCGCGATGCTCGGCATCGATGCGCTGGGTTACGAGCCCGACCAGAAAAAGGTGCTCATGGATGCGATC
GAACGCCCATACGGGATGGTTCTTGTCACCGGACCGACCGGTTCTGGCAAGACCGTTTCGCTCTACACCTGTCTCAATAT
CCTGAACAAGCCCGGGGTCAACATCTCGACGGCCGAGGATCCCGCAGAAATCAACCTGCCCGGCATCAACCAGGTCAACG
TCAACGAAAAAGCCGGCCTCACGTTCGCGGCGTCTCTTCGTTCGTTTCTGCGCCAGGATCCGGACGTCATCATGGTGGGC
GAAATCCGAGACCTGGAAACCGCCGAGATTTCCGTCAAGGCCGCGCAGACCGGCCACCTCGTCCTCTCGACGCTGCATAC
CAACGATGCGCCCACGACGCTGGAACGCCTCAAAAACATGGGCGTCGCGCCTTTCAACATTGCATCGTCCGTGATCATGA
TCACCGCGCAGCGACTTGCGCGCCGCCTGTGCACCTGCAAGCAACCGGTGGACATTCCCATCGAGGCCTTGCTGCAAGCC
GGTTTCAGCGGCGACGAACTCGACGGCAGCTGGCGCCCCTATGGCCCGGTCGGCTGCGAACGATGCAAGGGGAGCGGCTA
CAAGGGCCGGCTCGGCATCTACCAGGTCATGCCGATCAGCGACGAGATCGCACATATCATCATGACGAACGGCAATTCGA
TCGACATTGCCGCACAAGCACAACGGGAAGGCGTCCGCGACCTGCGACAGTCCGGACTCCTGAAGGTCAAGCAGGGGGCC
ACTTCGCTTGAAGAAGTGCTCGCCACTACAAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-148)

Type II/IV secretion system protein (T2SSE)

(195-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB H0Q417

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.634

99.301

0.552
  pilB Acinetobacter baylyi ADP1

55.732

99.126

0.552
  pilF Neisseria gonorrhoeae MS11

53.521

99.301

0.531
  pilB Legionella pneumophila strain ERS1305867

51.323

99.126

0.509
  pilB Vibrio cholerae strain A1552

49.088

95.804

0.47
  pilB Vibrio parahaemolyticus RIMD 2210633

46.585

99.825

0.465
  pilB Vibrio campbellii strain DS40M4

46.181

98.427

0.455
  pilF Thermus thermophilus HB27

40.11

95.455

0.383
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.789

93.007

0.379
  pilB/pilB1 Synechocystis sp. PCC 6803

40.076

92.483

0.371

Multiple sequence alignment