Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KHW14_RS02460 Genome accession   NZ_CP074412
Coordinates   548412..550511 (+) Length   699 a.a.
NCBI ID   WP_043305133.1    Uniprot ID   -
Organism   Pseudomonas viridiflava strain U625     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 543412..555511
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHW14_RS02440 (KHW14_02440) pilM 545578..546642 (+) 1065 WP_122421621.1 pilus assembly protein PilM Machinery gene
  KHW14_RS02445 (KHW14_02445) - 546642..547220 (+) 579 WP_122433130.1 PilN domain-containing protein -
  KHW14_RS02450 (KHW14_02450) pilO 547217..547840 (+) 624 WP_069143009.1 type 4a pilus biogenesis protein PilO -
  KHW14_RS02455 (KHW14_02455) - 547837..548364 (+) 528 WP_029243168.1 pilus assembly protein PilP -
  KHW14_RS02460 (KHW14_02460) pilQ 548412..550511 (+) 2100 WP_043305133.1 type IV pilus secretin PilQ Machinery gene
  KHW14_RS02465 (KHW14_02465) aroK 550516..551034 (+) 519 WP_004882714.1 shikimate kinase AroK -
  KHW14_RS02470 (KHW14_02470) aroB 551160..552263 (+) 1104 WP_004882713.1 3-dehydroquinate synthase -
  KHW14_RS02475 (KHW14_02475) - 552273..553901 (+) 1629 WP_122556519.1 AAA family ATPase -

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 75343.73 Da        Isoelectric Point: 5.0501

>NTDB_id=564734 KHW14_RS02460 WP_043305133.1 548412..550511(+) (pilQ) [Pseudomonas viridiflava strain U625]
MTRILSIIGVSLWIAMMSPVLQAANLKTLDVAALPGDRIELKLSFDAPVPAPRGYTTEQPARIALDLPGVTSQLATKSRD
LGAGNARSVVVVQAQDRTRVIINLTTLSPYSSRVDGNNLFVVIGQGAAAAQASQPTTAKGAARVAVPPPTPASARPYVPA
GVRAIRNVDFQRGELGEGNVVIDLSNASIAADIQEQGGKIRVDFAKTQLPDPLRVRLDVKDFATPVQFVSATASGDKASI
SIEPSGAFDYSAFQTENKLTISVRPLTNEDVDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGSVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKADERGSITVDERTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKQIGVRWGGRTDRSRKW
SVGGLDDNGDEAGNTGNDLTANIPFVDLGAPNASAGVGIGFLTNNALLDLELSAMEKTGNGEIVSQPKVVTSDKETAKIL
KGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGETIV
IGGVFSNTQSKVVEKVPFLGDVPYLGRLFRRDVVAEAKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=564734 KHW14_RS02460 WP_043305133.1 548412..550511(+) (pilQ) [Pseudomonas viridiflava strain U625]
ATGACCCGGATTCTCTCGATTATCGGCGTTTCGTTATGGATAGCGATGATGTCGCCGGTTCTCCAGGCCGCCAATCTCAA
GACGCTCGACGTCGCGGCCCTGCCGGGTGATCGAATCGAGCTCAAGCTTTCCTTCGACGCTCCGGTGCCCGCGCCACGCG
GCTATACCACCGAGCAGCCTGCGCGCATTGCGCTGGACCTGCCGGGCGTGACCAGCCAGCTGGCGACCAAGAGTCGTGAT
CTGGGCGCAGGCAATGCCCGCAGTGTGGTGGTTGTGCAGGCGCAGGACCGTACGCGAGTCATCATCAACCTGACGACGCT
TTCGCCGTACAGCTCACGCGTCGATGGCAACAACTTGTTCGTGGTCATCGGTCAGGGTGCGGCGGCGGCACAGGCTTCGC
AGCCGACCACCGCCAAGGGCGCTGCACGGGTCGCCGTGCCGCCGCCCACACCGGCATCGGCGCGGCCCTACGTGCCTGCG
GGCGTCAGAGCCATTCGCAATGTTGACTTCCAGCGCGGCGAGCTGGGCGAAGGCAACGTGGTCATCGACCTGAGCAATGC
CAGCATCGCTGCGGATATTCAGGAGCAGGGCGGCAAGATCCGTGTCGACTTCGCCAAGACGCAATTGCCCGATCCGCTGC
GCGTGCGTCTGGACGTGAAAGACTTTGCCACACCCGTGCAATTCGTCAGCGCCACAGCGTCTGGCGACAAGGCCAGCATT
TCCATCGAGCCATCGGGTGCGTTCGATTATTCGGCGTTCCAGACCGAAAACAAACTGACCATCAGCGTGCGCCCATTGAC
CAATGAGGACGTGGATCGTCGCGCCGCCGACAAGCCGGTCTACACCGGCGAGAAGCTTTCGCTGAATTTCCAGGACATCG
ATGTGCGTTCGGTACTGCAGCTGATCGCCGATTTCACCAACCTCAATCTGGTTGCCAGTGACACGGTTCAGGGCGGCATC
ACCCTGCGTCTGCAGAACGTGCCGTGGGATCAGGCGCTGGACCTGGTGCTCAAGACCAAGGGGCTGGACAAGCGCAAGGT
CGGCAGCGTGTTGCTGGTCGCCCCGGCTGACGAAATCGCCGCGCGTGAGCGTCAGGAGCTGGAGTCGCTCAAGCAGATTG
CCGAACTGGCCCCGTTGCGTCGTGAGCTGCTGCAGGTCAACTACGCCAAGGCTGCGGACATCGCCAAGCTGTTCCAGTCG
GTCACCAGCGCCGAGTCCAAGGCGGACGAGCGTGGTTCCATTACTGTGGATGAGCGCACCAACAACATCATCGCCTACCA
GACTCAGGATCGACTCGACGAGCTGCGCCGTATCGTCTCGCAGCTGGATATTCCGGTACGTCAGGTGATGATCGAGGCGC
GAATCGTCGAAGCCAACGTCGACTATGACAAGCAGATCGGCGTGCGCTGGGGCGGGCGAACCGACCGGTCGCGCAAGTGG
AGCGTGGGCGGTCTCGACGATAACGGCGATGAAGCAGGCAACACGGGCAACGATCTGACGGCCAACATTCCATTTGTGGA
TCTGGGTGCTCCTAATGCCTCTGCGGGTGTAGGCATCGGTTTCCTGACCAATAATGCGCTGCTTGACCTTGAGCTGAGTG
CGATGGAGAAGACCGGCAACGGTGAAATCGTCTCCCAGCCCAAGGTCGTCACCTCCGACAAGGAAACCGCGAAGATCCTC
AAGGGCACAGAGATTCCGTATCAGGAATCCAGCTCCAGCGGCGCCACGACAGTGAGCTTCAAGGAGGCCTCCCTGTCGCT
TGAGGTCACGCCACAGATTACGCCGGACAACCGCATCATCATGGAGGTCAAGGTTACCAAGGACGAGCCTGATTACCTCA
ATGCGGTGCTGGGCGTGCCTCCTATCAAGAAGAACGAGGTCAACGCCAAGGTGCTGATTTCCGACGGCGAGACCATCGTG
ATCGGTGGTGTGTTCTCCAATACGCAAAGTAAAGTTGTAGAGAAAGTGCCATTTCTCGGTGATGTGCCGTATCTTGGCCG
CCTTTTCAGGCGCGACGTGGTGGCAGAGGCGAAATCCGAGCTGTTGGTATTTCTGACACCGCGTATCATGAACAACCAGG
CGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(309-355)

Bacterial type II and III secretion system protein (Secretin)

(534-690)

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

AMIN domain (AMIN)

(37-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.122

100

0.744
  comQ Acinetobacter baylyi ADP1

41.026

100

0.412
  pilQ Acinetobacter baumannii strain A118

37.893

100

0.396
  pilQ Acinetobacter baumannii D1279779

37.893

100

0.396
  pilQ Legionella pneumophila strain Lp02

38.214

97.711

0.373
  pilQ Legionella pneumophila strain ERS1305867

38.214

97.711

0.373