Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   KHW14_RS02440 Genome accession   NZ_CP074412
Coordinates   545578..546642 (+) Length   354 a.a.
NCBI ID   WP_122421621.1    Uniprot ID   -
Organism   Pseudomonas viridiflava strain U625     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 540578..551642
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHW14_RS02430 (KHW14_02430) - 542124..542579 (-) 456 WP_004882721.1 YkgJ family cysteine cluster protein -
  KHW14_RS02435 (KHW14_02435) - 542927..545371 (-) 2445 WP_213674096.1 penicillin-binding protein 1A -
  KHW14_RS02440 (KHW14_02440) pilM 545578..546642 (+) 1065 WP_122421621.1 pilus assembly protein PilM Machinery gene
  KHW14_RS02445 (KHW14_02445) - 546642..547220 (+) 579 WP_122433130.1 PilN domain-containing protein -
  KHW14_RS02450 (KHW14_02450) pilO 547217..547840 (+) 624 WP_069143009.1 type 4a pilus biogenesis protein PilO -
  KHW14_RS02455 (KHW14_02455) - 547837..548364 (+) 528 WP_029243168.1 pilus assembly protein PilP -
  KHW14_RS02460 (KHW14_02460) pilQ 548412..550511 (+) 2100 WP_043305133.1 type IV pilus secretin PilQ Machinery gene
  KHW14_RS02465 (KHW14_02465) aroK 550516..551034 (+) 519 WP_004882714.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38058.48 Da        Isoelectric Point: 4.7090

>NTDB_id=564733 KHW14_RS02440 WP_122421621.1 545578..546642(+) (pilM) [Pseudomonas viridiflava strain U625]
MFELFGKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVVGKAKTNARIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSLRNPERVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTEEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLEPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRLGTPTQVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=564733 KHW14_RS02440 WP_122421621.1 545578..546642(+) (pilM) [Pseudomonas viridiflava strain U625]
GTGTTCGAACTCTTCGGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGTTCCACCTCGGTCAAGCTCCTTGAATT
GAGTCGTTCCGGAACCCGCTACAAAGTCGAGTCTTACGCTGTTGAGCCGTTGCCGGCCAATGCCGTCGTCGAGAAGAACA
TTGCCGAACTCGAAGGGGTTGGTCAGGCGTTGTCACGGGTCGTTGGCAAGGCCAAGACCAACGCCAGAATAGTGGCGGTC
GCTGTCGCAGGCTCCGCAGTCATCACCAAGACGATCGAAATGGATGCCGGTCTCTCGGACGACGACATGGAAAATCAGCT
CAAGCTCGAGGCTGACCAGTACATCCCCTATCCCCTTGAAGAAGTGGCCATCGATTTCGAAGTGCAGGGCTATTCGCTGC
GCAACCCCGAACGCGTGGAAGTCCTTCTGGCTGCCTGCCGCAAGGAAAACGTCGAGGTGCGCGAGGCCGCGCTGGCGCTG
GCTGGCCTCACCGCAAAAGTCGTTGATGTCGAAGCCTATGCCCTTGAGCGTTCGTTCGGGCTGCTGGCTGCGCAACTGGG
CAATGGTCACGATGAGCTGACCGTTGCGGTCGTCGATATCGGCGCGACCATGACCACACTGAGCGTTCTGCACCACGGCC
GGATCATCTACACCCGCGAGCAACTGTTCGGCGGACGCCAACTGACCGAAGAAATCCAGCGTCGCTACGGCCTGTCGATG
GAAGAGGCCGGGCTTGCCAAGAAACAGGGCGGGCTGCCGGATGACTATGTCAGTGAAGTGCTGGAACCCTTCAAGGATGC
GCTGGTGCAGCAGGTCTCGCGCTCTCTTCAATTCTTCTTCGCGGCAGGCCAGTACAACTCTGTGGATCACATCATGCTCG
CTGGTGGTACGGCTTCCATTTCCGGCCTGGAGCATCTGATTCAGCGCCGACTCGGCACGCCGACCCAGGTGGCCAACCCG
TTTGCCGACATGGCCCTCAGTTCCAAGGTCAACGCCGGAGCGCTGGCCAGTGATGCGCCCGCGCTGATGATCGCGTGCGG
TCTTGCTTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.78

100

0.568
  comM Acinetobacter nosocomialis M2

56.78

100

0.568
  comM Acinetobacter baylyi ADP1

55.085

100

0.551
  pilM Legionella pneumophila strain ERS1305867

47.887

100

0.48