Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KHW12_RS01635 Genome accession   NZ_CP074409
Coordinates   352446..354551 (+) Length   701 a.a.
NCBI ID   WP_024665233.1    Uniprot ID   -
Organism   Pseudomonas syringae strain KF529     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 347446..359551
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHW12_RS01615 (KHW12_01615) pilM 349609..350673 (+) 1065 WP_003341394.1 pilus assembly protein PilM Machinery gene
  KHW12_RS01620 (KHW12_01620) - 350673..351254 (+) 582 WP_003316651.1 PilN domain-containing protein -
  KHW12_RS01625 (KHW12_01625) pilO 351251..351874 (+) 624 WP_003316652.1 type 4a pilus biogenesis protein PilO -
  KHW12_RS01630 (KHW12_01630) pilP 351871..352398 (+) 528 WP_003316653.1 type 4a pilus biogenesis lipoprotein PilP -
  KHW12_RS01635 (KHW12_01635) pilQ 352446..354551 (+) 2106 WP_024665233.1 type IV pilus secretin PilQ Machinery gene
  KHW12_RS01640 (KHW12_01640) aroK 354556..355074 (+) 519 WP_003341387.1 shikimate kinase AroK -
  KHW12_RS01645 (KHW12_01645) aroB 355190..356293 (+) 1104 WP_024665234.1 3-dehydroquinate synthase -
  KHW12_RS01650 (KHW12_01650) - 356304..357920 (+) 1617 WP_003316656.1 AAA family ATPase -

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 75454.84 Da        Isoelectric Point: 5.1826

>NTDB_id=564646 KHW12_RS01635 WP_024665233.1 352446..354551(+) (pilQ) [Pseudomonas syringae strain KF529]
MTRILSIIGVSLCIAMLSPVLQAANLKTLDVAALPGDRIELKLAFDAPVPAPRGYTTSQPARIALDLPGVTSQLATKSRD
LGSGNARSVVVVQAQDRTRVIINLTTLSPYSSRVDGNNLFVVIGQGAGAAQASQPTTAMGAQRVAVPPPTPASARPFIPA
GVKAIRNVDFQRGELGEGNVVIDLSNPSIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFATPVQFVSASASGDKASI
LIEPSGAFDYSAFQTENKLTISVRPLTNEDVDRRAADKPAYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKADERGSITVDDRTNNIIAYQTQERLDELRRIVSQLDIPVRQVMIEARIVEANVDYNKQLGVRWGGSTNTSGNR
KWTTYGLDNNGDEAGNTSGNLTPNVPFVDLGAAGATSGIGLGFVTNNTLLDLELSAMEKTGNGEIVSQPKVVTSDKETAK
ILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGET
IVIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=564646 KHW12_RS01635 WP_024665233.1 352446..354551(+) (pilQ) [Pseudomonas syringae strain KF529]
ATGACCCGGATTCTCTCGATTATCGGCGTGTCGTTATGCATCGCGATGCTGTCGCCGGTTCTCCAGGCTGCCAACCTCAA
GACCCTGGATGTCGCCGCCCTGCCGGGTGACCGCATCGAGCTGAAGCTGGCATTCGATGCCCCGGTGCCTGCACCGCGCG
GTTACACCACGTCGCAACCGGCGCGCATTGCGCTGGATCTGCCGGGTGTTACCAGCCAGCTGGCGACCAAAAGTCGTGAT
CTGGGCAGCGGCAATGCGCGCAGTGTGGTTGTGGTTCAGGCTCAGGACCGCACTCGGGTGATCATCAACCTGACCACGCT
TTCGCCTTACAGCTCCCGCGTCGATGGCAACAACCTGTTCGTGGTCATCGGCCAGGGTGCCGGCGCCGCGCAAGCCTCCC
AGCCGACCACTGCGATGGGCGCGCAACGGGTCGCGGTGCCGCCGCCCACGCCGGCATCCGCCAGGCCGTTCATCCCCGCT
GGCGTCAAGGCCATCAGGAACGTCGATTTCCAGCGTGGCGAGCTGGGTGAAGGCAATGTGGTCATCGACCTGAGCAACCC
CAGCATTGCCCCGGATATTCAGGAGCAGGGCGGCAAGATCCGCGTGGACTTCGCCAAGACCCAACTGCCCGAACCGCTGC
GCGTCCGCCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTCGTCAGTGCTTCGGCGTCGGGTGACAAGGCCAGCATT
CTGATCGAGCCGTCGGGCGCCTTCGACTACTCGGCGTTTCAGACCGAAAACAAGCTGACGATCAGCGTGCGTCCATTGAC
CAACGAGGACGTCGACCGCCGCGCCGCTGACAAACCGGCGTACACCGGTGAAAAGCTGTCGCTGAACTTCCAGGACATCG
ACGTGCGTTCGGTGCTGCAGCTGATTGCCGACTTCACCAACCTCAATCTGGTGGCCAGCGATACGGTCCAGGGTGGTATC
ACCCTGCGTCTGCAGAATGTGCCGTGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAGGGCCTGGACAAACGCAAGGT
CGGCAACGTGCTGCTGGTTGCGCCAGCCGATGAAATTGCCGCCCGCGAGCGTCAGGAGCTGGAGTCGCTCAAGCAGATCG
CCGAGCTGGCGCCCCTGCGCCGTGAGCTGTTGCAGGTCAATTACGCCAAGGCTGCCGACATCGCCAAGCTGTTCCAGTCT
GTGACCAGTGCCGAGTCCAAGGCCGATGAGCGCGGCTCGATCACGGTGGACGACCGGACCAATAACATCATTGCCTACCA
GACCCAGGAACGACTCGACGAACTGCGTCGTATCGTTTCGCAACTGGACATCCCGGTACGCCAGGTGATGATCGAGGCGC
GCATCGTCGAGGCCAACGTCGATTACAACAAGCAGCTCGGGGTTCGCTGGGGCGGCTCTACGAACACCAGCGGTAACAGA
AAATGGACAACCTACGGGCTTGACAATAATGGCGACGAGGCCGGTAACACCAGCGGCAACCTGACCCCCAACGTGCCGTT
CGTGGACCTGGGCGCAGCCGGCGCCACCTCGGGTATCGGCCTTGGTTTCGTGACCAACAACACGTTGCTGGACCTTGAAC
TGAGTGCCATGGAGAAAACCGGCAACGGTGAAATCGTCTCCCAGCCCAAGGTGGTCACTTCCGACAAGGAAACCGCCAAG
ATCCTCAAGGGTACGGAGATCCCGTATCAGGAATCCAGCTCCAGCGGTGCGACCACCGTGAGCTTCAAGGAGGCTTCCCT
GTCGCTTGAAGTGACGCCGCAGATCACGCCGGACAATCGCATCATCATGGAAGTGAAGGTCACCAAGGACGAGCCGGACT
ACCTGAATGCGGTGCTGGGTGTGCCGCCGATCAAGAAGAACGAGGTCAACGCCAAAGTGCTGATTTCCGACGGCGAGACC
ATCGTCATCGGTGGTGTGTTCTCGAATACGCAAAGTAAAGTTGTCGATAAAGTGCCATTTCTTGGCGATGTACCGTATCT
TGGCCGGCTTTTCAGGCGCGATGTGGTATCTGAGTCCAAATCCGAGCTGTTGGTATTTCTGACTCCGCGTATCATGAACA
ACCAGGCGATTTCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(309-355)

Bacterial type II and III secretion system protein (Secretin)

(536-692)

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

AMIN domain (AMIN)

(38-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.056

100

0.75
  comQ Acinetobacter baylyi ADP1

41.917

99.715

0.418
  pilQ Acinetobacter baumannii D1279779

38.587

100

0.405
  pilQ Acinetobacter baumannii strain A118

38.587

100

0.405
  pilQ Legionella pneumophila strain ERS1305867

38.061

98.573

0.375
  pilQ Legionella pneumophila strain Lp02

38.026

98.288

0.374