Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   KHW12_RS01615 Genome accession   NZ_CP074409
Coordinates   349609..350673 (+) Length   354 a.a.
NCBI ID   WP_003341394.1    Uniprot ID   A0A0P9GH14
Organism   Pseudomonas syringae strain KF529     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 344609..355673
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHW12_RS01605 (KHW12_01605) - 344856..346124 (+) 1269 WP_003316648.1 malic enzyme-like NAD(P)-binding protein -
  KHW12_RS01610 (KHW12_01610) - 346958..349402 (-) 2445 WP_032663001.1 penicillin-binding protein 1A -
  KHW12_RS01615 (KHW12_01615) pilM 349609..350673 (+) 1065 WP_003341394.1 pilus assembly protein PilM Machinery gene
  KHW12_RS01620 (KHW12_01620) - 350673..351254 (+) 582 WP_003316651.1 PilN domain-containing protein -
  KHW12_RS01625 (KHW12_01625) pilO 351251..351874 (+) 624 WP_003316652.1 type 4a pilus biogenesis protein PilO -
  KHW12_RS01630 (KHW12_01630) pilP 351871..352398 (+) 528 WP_003316653.1 type 4a pilus biogenesis lipoprotein PilP -
  KHW12_RS01635 (KHW12_01635) pilQ 352446..354551 (+) 2106 WP_024665233.1 type IV pilus secretin PilQ Machinery gene
  KHW12_RS01640 (KHW12_01640) aroK 354556..355074 (+) 519 WP_003341387.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38134.68 Da        Isoelectric Point: 4.7090

>NTDB_id=564645 KHW12_RS01615 WP_003341394.1 349609..350673(+) (pilM) [Pseudomonas syringae strain KF529]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLVKAKTSVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLEPFKEALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=564645 KHW12_RS01615 WP_003341394.1 349609..350673(+) (pilM) [Pseudomonas syringae strain KF529]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACCCGTTACAAGGTCGAGTCTTACGCAGTAGAGCCATTGCCGGCCAACGCGGTCGTCGAAAAGAACA
TTGCCGAACTCGAAGGGGTCGGGCAGGCGTTGTCACGCGTGCTCGTCAAGGCCAAGACCAGCGTCAAGATCGTGGCGGTT
GCCGTGGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGACGCCGGGCTTTCCGACGATGACATGGAAAACCAGCT
CAAGCTTGAAGCTGACCAGTACATCCCCTATCCGCTGGAAGAAGTCGCCATCGATTTCGAAGTGCAGGGCTATTCGGTGC
GCAACCCCGAACGCGTCGAGGTGTTACTGGCTGCCTGCCGCAAGGAAAACGTCGAAGTACGCGAGGCCGCGCTGGCACTG
GCCGGGCTGACCGCACGCGTCGTCGATGTCGAGGCCTACGCCCTTGAGCGTTCGTTCGGTTTGCTGGCGGCGCAACTGGG
CAATGGCCACGACGAGCTGACGGTGGCCGTGGTCGATATCGGCGCCACCATGACCACCCTGAGCGTGCTGCATCATGGCC
GGATCATCTATACCCGTGAGCAGCTGTTTGGTGGGCGCCAGTTGACTGACGAAATCCAGCGCCGCTACGGGCTCTCCATG
GAGGAAGCCGGGCTTGCCAAGAAGCAGGGCGGCTTGCCCGACGATTACGTCAGTGAGGTGCTCGAGCCCTTCAAGGAAGC
GCTGGTGCAGCAGGTTTCCCGATCCCTGCAGTTCTTCTTCGCGGCCGGTCAATACAACTCAGTGGATCACATCATGCTGG
CAGGCGGCACGGCCTCGATTTCAGGGCTTGAGCATCTGATCCAGCGGCGCATCGGCACGCCGACCATGGTCGCCAACCCG
TTTGCCGATATGGCACTGAGCTCCAAGGTCAATGCCGGCGCCCTGGCCAGTGATGCGCCGGCGCTGATGATCGCGTGCGG
CCTGGCGTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0P9GH14

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.65

100

0.557
  comM Acinetobacter nosocomialis M2

55.65

100

0.557
  comM Acinetobacter baylyi ADP1

54.237

100

0.542
  pilM Legionella pneumophila strain ERS1305867

46.761

100

0.469