Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KGD89_RS01875 Genome accession   NZ_CP074349
Coordinates   399899..401998 (+) Length   699 a.a.
NCBI ID   WP_025258136.1    Uniprot ID   -
Organism   Pseudomonas cichorii strain DSM 50259     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 394899..406998
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KGD89_RS01855 (KGD89_01855) pilM 397071..398135 (+) 1065 WP_025258132.1 pilus assembly protein PilM Machinery gene
  KGD89_RS01860 (KGD89_01860) - 398135..398707 (+) 573 WP_025258133.1 PilN domain-containing protein -
  KGD89_RS01865 (KGD89_01865) pilO 398704..399327 (+) 624 WP_025258134.1 type 4a pilus biogenesis protein PilO -
  KGD89_RS01870 (KGD89_01870) - 399324..399851 (+) 528 WP_025258135.1 pilus assembly protein PilP -
  KGD89_RS01875 (KGD89_01875) pilQ 399899..401998 (+) 2100 WP_025258136.1 type IV pilus secretin PilQ Machinery gene
  KGD89_RS01880 (KGD89_01880) aroK 402003..402521 (+) 519 WP_025258137.1 shikimate kinase AroK -
  KGD89_RS01885 (KGD89_01885) aroB 402637..403740 (+) 1104 WP_025258138.1 3-dehydroquinate synthase -
  KGD89_RS01890 (KGD89_01890) - 403751..405376 (+) 1626 WP_025258139.1 AAA family ATPase -

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 75172.40 Da        Isoelectric Point: 4.8779

>NTDB_id=564113 KGD89_RS01875 WP_025258136.1 399899..401998(+) (pilQ) [Pseudomonas cichorii strain DSM 50259]
MIRILSIIGVSLGIAMLSPVLQAANLKTLDVAALPGDRIELKLSFDSPVPAPRGYTTEQPARIALDLPGVTSQLATKSRD
LGAGNARSVVVVQAQDRTRVIINMTTLSPYSSRVEGNNLFVVVGQGAAAAQSSQPSTAMGTPRVAVPTPAPSAARPYVPA
GVKAIRNIDFQRGELGEGNVVIDLSSPGISPDIQEQGGKIRIDFAKTQLPEPLRVRLDVKDFATPVQFVSATTSGDKASI
SIEPSGSFDYSAYQTENKLTISVRPLTNEDLDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGSVLLVAPADEIAARERQELESLKQISELAPLRRELLQVNYAKAADIAKLFQS
VTSVESKADERGSITVDERTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKALGVRWGGTKVGSGNW
NTYGLDDNGDEAGNTGSDLSANTPFVDLGAGGATSGIGIAFVTNNTLLDLELSAMEKTGNGEIVSQPKVVTSDKETAKIL
KGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLVSDGETIV
IGGVFSNTQSKVVEKVPFLGDVPYLGRLFRRDTVSEAKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=564113 KGD89_RS01875 WP_025258136.1 399899..401998(+) (pilQ) [Pseudomonas cichorii strain DSM 50259]
ATGATCAGGATTCTCTCGATTATCGGCGTTTCGCTAGGGATAGCGATGCTTTCGCCGGTTCTCCAGGCAGCCAATCTTAA
AACGCTGGATGTTGCAGCCTTGCCAGGCGACCGGATCGAGCTGAAGCTTTCCTTCGATTCTCCGGTTCCTGCGCCGCGCG
GTTATACCACCGAACAGCCGGCGCGCATCGCGCTGGACCTGCCGGGTGTGACCAGCCAGCTGGCAACCAAGAGTCGTGAT
CTGGGTGCGGGCAATGCGCGCAGTGTCGTGGTGGTTCAGGCCCAGGACCGCACGCGGGTCATCATCAACATGACGACGCT
GTCTCCTTACAGCTCCCGAGTCGAAGGCAACAACCTGTTCGTGGTGGTCGGCCAGGGCGCTGCCGCTGCGCAATCCTCCC
AGCCGAGCACGGCCATGGGAACACCGCGAGTCGCCGTCCCGACGCCCGCTCCGTCAGCGGCACGGCCTTACGTGCCGGCA
GGTGTGAAAGCCATCAGGAATATCGACTTCCAGCGCGGCGAGCTGGGTGAAGGCAATGTAGTCATCGACCTGAGCAGTCC
TGGCATTTCTCCGGACATTCAGGAGCAGGGCGGCAAGATTCGTATCGACTTTGCCAAGACCCAGTTGCCTGAGCCGCTGC
GAGTCAGGCTGGACGTCAAGGATTTCGCCACGCCTGTGCAGTTCGTGAGTGCCACGACCTCCGGCGACAAGGCGAGCATC
AGCATCGAGCCTTCGGGCTCCTTCGATTACTCGGCCTACCAGACTGAAAACAAGCTGACCATCAGCGTGCGCCCCCTGAC
CAATGAAGACCTGGATCGCCGTGCTGCCGACAAGCCGGTCTACACCGGTGAAAAACTGTCGCTGAATTTCCAGGACATCG
ATGTGCGCTCGGTGCTGCAACTGATTGCCGACTTCACTAACCTGAATCTGGTGGCCAGTGACACGGTTCAGGGCGGTATC
ACCCTGCGCCTGCAGAACGTGCCGTGGGATCAGGCGCTGGATCTGGTGCTCAAGACCAAGGGCCTCGACAAGCGCAAGGT
CGGCAGTGTCCTGCTGGTCGCGCCAGCCGATGAAATCGCCGCCCGTGAGCGTCAGGAGCTGGAATCCCTCAAACAGATTT
CCGAACTGGCGCCGTTACGGCGCGAGCTTCTGCAGGTCAATTACGCCAAGGCGGCCGACATCGCCAAGCTGTTCCAGTCG
GTGACCAGCGTCGAATCGAAAGCCGACGAACGCGGTTCGATCACTGTGGATGAGCGTACCAACAATATCATTGCCTACCA
GACCCAGGATCGCCTCGACGAGCTGCGTCGCATCGTGTCCCAGCTGGATATTCCTGTGCGTCAGGTGATGATCGAGGCGC
GCATCGTTGAAGCCAACGTCGATTACGACAAGGCTCTGGGAGTGCGCTGGGGTGGTACCAAGGTTGGCAGTGGCAACTGG
AATACCTACGGTCTGGACGATAACGGCGACGAGGCGGGCAATACTGGCAGCGACCTGAGCGCCAACACGCCATTCGTCGA
CCTGGGTGCCGGTGGCGCGACCTCCGGGATCGGCATCGCCTTCGTCACCAACAATACCCTGCTCGATCTCGAGTTGAGCG
CAATGGAAAAGACCGGCAACGGTGAAATCGTTTCCCAGCCCAAGGTGGTCACTTCCGACAAGGAAACCGCCAAGATCCTC
AAAGGTACGGAAATCCCTTATCAGGAATCCAGCTCCAGCGGCGCCACAACCGTGAGTTTCAAGGAAGCGTCCCTGTCGCT
GGAAGTGACGCCGCAGATCACGCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTACCTCA
ATGCCGTGCTGGGTGTACCTCCGATCAAGAAAAACGAGGTCAATGCCAAGGTGCTGGTTTCCGATGGCGAGACGATCGTG
ATCGGTGGCGTGTTCTCCAACACACAAAGTAAAGTTGTAGAGAAAGTGCCATTTCTTGGCGATGTACCGTATCTTGGCCG
CCTTTTCCGTCGAGATACGGTTTCAGAAGCGAAATCCGAGCTGTTGGTATTTCTGACTCCGCGTATCATGAACAACCAGG
CGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-121)

Bacterial type II and III secretion system protein (Secretin)

(534-690)

Secretin and TonB N terminus short domain (STN)

(309-355)

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

74.477

100

0.764
  comQ Acinetobacter baylyi ADP1

42.06

100

0.421
  pilQ Acinetobacter baumannii strain A118

39.726

100

0.415
  pilQ Acinetobacter baumannii D1279779

39.726

100

0.415
  pilQ Legionella pneumophila strain Lp02

38.382

97.282

0.373
  pilQ Legionella pneumophila strain ERS1305867

38.382

97.282

0.373