Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   KGD89_RS01855 Genome accession   NZ_CP074349
Coordinates   397071..398135 (+) Length   354 a.a.
NCBI ID   WP_025258132.1    Uniprot ID   -
Organism   Pseudomonas cichorii strain DSM 50259     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 392071..403135
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KGD89_RS01840 (KGD89_01840) - 392352..393206 (+) 855 WP_025258129.1 sulfurtransferase -
  KGD89_RS01845 (KGD89_01845) - 393258..394304 (-) 1047 WP_025258130.1 hydrolase -
  KGD89_RS01850 (KGD89_01850) - 394421..396859 (-) 2439 WP_371874242.1 penicillin-binding protein 1A -
  KGD89_RS01855 (KGD89_01855) pilM 397071..398135 (+) 1065 WP_025258132.1 pilus assembly protein PilM Machinery gene
  KGD89_RS01860 (KGD89_01860) - 398135..398707 (+) 573 WP_025258133.1 PilN domain-containing protein -
  KGD89_RS01865 (KGD89_01865) pilO 398704..399327 (+) 624 WP_025258134.1 type 4a pilus biogenesis protein PilO -
  KGD89_RS01870 (KGD89_01870) - 399324..399851 (+) 528 WP_025258135.1 pilus assembly protein PilP -
  KGD89_RS01875 (KGD89_01875) pilQ 399899..401998 (+) 2100 WP_025258136.1 type IV pilus secretin PilQ Machinery gene
  KGD89_RS01880 (KGD89_01880) aroK 402003..402521 (+) 519 WP_025258137.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38127.62 Da        Isoelectric Point: 4.6928

>NTDB_id=564112 KGD89_RS01855 WP_025258132.1 397071..398135(+) (pilM) [Pseudomonas cichorii strain DSM 50259]
MFELFGKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLVKAKTNAKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSARNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLSAQLGNDHDQLTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTEEIQRRYGLSM
EEAGLAKKQGGLSDDYVSEVLNPFKDALVQQVSRSLQFFFAAGQYNTVDYIMLAGGTASIPGLEHLIQKRLGTPTLVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=564112 KGD89_RS01855 WP_025258132.1 397071..398135(+) (pilM) [Pseudomonas cichorii strain DSM 50259]
GTGTTCGAACTCTTCGGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCTACCTCGGTAAAACTCCTTGAGTT
GAGTCGTTCCGGCACTCGTTACAAGGTCGAGTCTTATGCGGTCGAACCGCTGCCGGCCAATGCCGTCGTCGAAAAGAATA
TTGCCGAACTCGAAGGGGTTGGACAGGCGTTGTCGCGCGTGCTGGTCAAGGCCAAGACGAACGCCAAAATAGTGGCGGTT
GCTGTGGCGGGCTCGGCGGTCATCACCAAGACCATCGAGATGGACGCCGGTCTGTCCGACGACGACATGGAAAACCAGCT
CAAGCTTGAAGCTGACCAGTACATTCCCTATCCCCTCGAAGAAGTCGCCATCGATTTTGAAGTCCAGGGCTATTCGGCTC
GCAATCCCGAGCGCGTCGAAGTGCTGCTGGCCGCCTGCCGCAAGGAAAACGTGGAAGTACGTGAGGCCGCTTTGGCGCTG
GCCGGGCTGACGGCCCGTGTCGTCGACGTGGAGGCCTATGCACTGGAGCGTTCTTTCGGTCTGCTGTCTGCCCAACTGGG
CAATGACCACGATCAACTGACCGTTGCCGTGGTGGACATCGGCGCAACCATGACCACCCTGAGCGTGCTGCATCACGGGC
GCATCATCTATACCCGCGAGCAATTGTTCGGTGGGCGCCAGCTGACCGAGGAAATTCAGCGTCGCTATGGCCTGTCCATG
GAAGAAGCGGGGCTTGCGAAGAAGCAGGGCGGGCTGTCCGACGATTACGTCAGTGAAGTGTTGAACCCCTTCAAGGATGC
TCTGGTGCAGCAAGTGTCTCGCTCGCTGCAGTTTTTCTTTGCTGCCGGGCAGTACAACACCGTCGACTACATCATGCTGG
CGGGCGGTACGGCTTCCATTCCGGGACTTGAGCACTTGATCCAGAAACGTCTTGGCACGCCAACCCTGGTCGCGAACCCT
TTTGCCGACATGGCCCTGAGCTCCAAGGTCAACGCCGGTGCATTGGCCAGTGATGCTCCCGCGTTGATGATCGCCTGCGG
TCTTGCCCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.367

100

0.554
  comM Acinetobacter nosocomialis M2

55.367

100

0.554
  comM Acinetobacter baylyi ADP1

53.107

100

0.531
  pilM Legionella pneumophila strain ERS1305867

46.61

100

0.466