Detailed information NTG0564083

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: KHA74_RS13155
• Genome accession: NZ_CP074348
• Coordinates: 2703456..2704070 (-)
• Length: 204 a.a.
• NCBI ID: WP_064107157.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain SW5
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2698456..2709070

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KHA74_RS13125 (KHA74_13035)	rpsT	2698759..2699025 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
KHA74_RS13130 (KHA74_13040)	holA	2699042..2700083 (-)	1042	Protein_2540	DNA polymerase III subunit delta	-
KHA74_RS13135 (KHA74_13045)	-	2700123..2700274 (-)	152	Protein_2541	hypothetical protein	-
KHA74_RS13140 (KHA74_13050)	-	2700315..2700449 (+)	135	WP_003152870.1	YqzM family protein	-
KHA74_RS13145 (KHA74_13055)	comEC	2700468..2702819 (-)	2352	WP_060674655.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
KHA74_RS13150 (KHA74_13060)	-	2702820..2703389 (-)	570	WP_003152868.1	ComE operon protein 2	-
KHA74_RS13155 (KHA74_13065)	comEA	2703456..2704070 (-)	615	WP_064107157.1	helix-hairpin-helix domain-containing protein	Machinery gene
KHA74_RS13160 (KHA74_13070)	comER	2704129..2704950 (+)	822	WP_064107158.1	late competence protein ComER	-
KHA74_RS13165 (KHA74_13075)	-	2705019..2705756 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
KHA74_RS13170 (KHA74_13080)	rsfS	2705753..2706109 (-)	357	WP_007408260.1	ribosome silencing factor	-
KHA74_RS13175 (KHA74_13085)	yqeK	2706127..2706687 (-)	561	WP_007408259.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
KHA74_RS13180 (KHA74_13090)	-	2706677..2707246 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
KHA74_RS13185 (KHA74_13095)	yhbY	2707257..2707547 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
KHA74_RS13190 (KHA74_13100)	aroE	2707541..2708383 (-)	843	WP_033574150.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22249.47 Da
• Isoelectric Point: 6.4888

>NTDB_id=564083 KHA74_RS13155 WP_064107157.1 2703456..2704070(-) (comEA) [Bacillus velezensis strain SW5]
MTKAMMTERLRRNLKILVACLAAGAIFICIWLFRSEKEEPVKQQSVLGQTASVSVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=564083 KHA74_RS13155 WP_064107157.1 2703456..2704070(-) (comEA) [Bacillus velezensis strain SW5]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATCCTGGTCGCTTGTCTTGCGGCAGGGGCGATTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTTGGCCAAACAGCCTCAGTAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	57.653	96.078	0.554
comEA	Lactococcus lactis subsp. cremoris KW2	35.135	100	0.382
comEA	Latilactobacillus sakei subsp. sakei 23K	33.19	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae Rx1	36.493	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae D39	36.493	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae R6	36.493	100	0.377
comEA/celA/cilE	Streptococcus mitis NCTC 12261	36.493	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.493	100	0.377
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363