Detailed information NTG0056387

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: BAM5036_RS11850
• Genome accession: NC_020410
• Coordinates: 2499878..2500492 (-)
• Length: 204 a.a.
• NCBI ID: WP_015417854.1
• Uniprot ID: -
• Organism: Bacillus velezensis UCMB5036
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2494878..2505492

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BAM5036_RS11825 (BAM5036_2305)	rpsT	2495181..2495447 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
BAM5036_RS11830 (BAM5036_2306)	holA	2495464..2496505 (-)	1042	Protein_2330	DNA polymerase III subunit delta	-
BAM5036_RS20330	-	2496545..2496696 (-)	152	Protein_2331	hypothetical protein	-
BAM5036_RS19975	-	2496737..2496871 (+)	135	WP_003152870.1	YqzM family protein	-
BAM5036_RS11840 (BAM5036_2307)	comEC	2496890..2499241 (-)	2352	WP_015417853.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
BAM5036_RS11845 (BAM5036_2308)	-	2499242..2499811 (-)	570	WP_003152868.1	ComE operon protein 2	-
BAM5036_RS11850 (BAM5036_2309)	comEA	2499878..2500492 (-)	615	WP_015417854.1	helix-hairpin-helix domain-containing protein	Machinery gene
BAM5036_RS11855 (BAM5036_2310)	comER	2500551..2501372 (+)	822	WP_015417855.1	late competence protein ComER	-
BAM5036_RS11860 (BAM5036_2311)	-	2501441..2502178 (-)	738	WP_015417856.1	class I SAM-dependent DNA methyltransferase	-
BAM5036_RS11865 (BAM5036_2312)	rsfS	2502175..2502531 (-)	357	WP_007408260.1	ribosome silencing factor	-
BAM5036_RS11870 (BAM5036_2313)	yqeK	2502549..2503109 (-)	561	WP_007408259.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
BAM5036_RS11875 (BAM5036_2314)	-	2503099..2503668 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
BAM5036_RS11880 (BAM5036_2315)	yhbY	2503679..2503969 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
BAM5036_RS11885 (BAM5036_2316)	aroE	2503963..2504805 (-)	843	WP_015417858.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22207.39 Da
• Isoelectric Point: 6.4888

>NTDB_id=56387 BAM5036_RS11850 WP_015417854.1 2499878..2500492(-) (comEA) [Bacillus velezensis UCMB5036]
MTKAMMTERLRRNLKILVACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=56387 BAM5036_RS11850 WP_015417854.1 2499878..2500492(-) (comEA) [Bacillus velezensis UCMB5036]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATCCTGGTCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	57.653	96.078	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	33.19	100	0.377
comEA	Lactococcus lactis subsp. cremoris KW2	34.685	100	0.377
comEA/celA/cilE	Streptococcus mitis NCTC 12261	36.019	100	0.373
comEA/celA/cilE	Streptococcus pneumoniae Rx1	36.019	100	0.373
comEA/celA/cilE	Streptococcus pneumoniae R6	36.019	100	0.373
comEA/celA/cilE	Streptococcus pneumoniae D39	36.019	100	0.373
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368