Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KFB96_RS12010 Genome accession   NZ_CP073760
Coordinates   2662230..2663945 (+) Length   571 a.a.
NCBI ID   WP_213461618.1    Uniprot ID   -
Organism   MAG: Thiocapsa sp. isolate M50B4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2657230..2668945
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFB96_RS11995 (KFB96_11995) - 2658458..2659135 (+) 678 WP_213461611.1 exonuclease domain-containing protein -
  KFB96_RS12000 (KFB96_12000) acs 2659285..2661231 (+) 1947 WP_213461613.1 acetate--CoA ligase -
  KFB96_RS12005 (KFB96_12005) - 2661378..2662211 (+) 834 WP_213461615.1 NYN domain-containing protein -
  KFB96_RS12010 (KFB96_12010) pilB 2662230..2663945 (+) 1716 WP_213461618.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KFB96_RS12015 (KFB96_12015) pilC 2663957..2665210 (+) 1254 WP_213461620.1 type II secretion system F family protein Machinery gene
  KFB96_RS12020 (KFB96_12020) pilD 2665382..2666260 (+) 879 WP_213461622.1 A24 family peptidase Machinery gene
  KFB96_RS12025 (KFB96_12025) coaE 2666398..2667015 (+) 618 WP_213461624.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63443.93 Da        Isoelectric Point: 5.1963

>NTDB_id=560536 KFB96_RS12010 WP_213461618.1 2662230..2663945(+) (pilB) [MAG: Thiocapsa sp. isolate M50B4]
MATDQTAKNKLSGLALRLVRDNLISEREAIAAYDDATRRRQSFVSYLVEQKLLDGRKIAVAASHEFGVPLLDLHALDLLN
LPVNLVDERLVRKHRALPIFRRGNRLFLALSDPTNDQALEEIRFNTGLATDAVLVEEDKLKAAIETAIEAQDTTMTELMD
ADLEKLEITPDDEDLREDADINIDDAPIVRYVNKVLVDAISAGASDIHFEPYEKFFRVRFRQDGMLREVASPPMNIAGRL
VARLKVMSRMNIAERRVPQDGRIKLKVSRTRDIDFRVNTLPTLYGEKVVLRILDSASAQVGIEALGFELEQREAFLEAIH
KPYGMILVTGPTGSGKTVSLYTALNLLNQPDINISTVEDPVEIQVPGINQVSMNPKTGLTFAAALRAFLRQDPDIVMVGE
IRDLETAEIAVKAAQTGHLVLSTLHTNDAPQSLTRLANMGVAPFNIASSVLLIMAQRLARRLCPTCKAPEDLPREVLRQE
GFNEADIEEGIVVFGPVGCERCTKGYRGRVGIFQVMPISETIRRLILEGGNAMQLAEQARREGVADLRESGLRKVKEGMT
SLEELNRVTKE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=560536 KFB96_RS12010 WP_213461618.1 2662230..2663945(+) (pilB) [MAG: Thiocapsa sp. isolate M50B4]
ATGGCGACGGACCAAACAGCCAAGAACAAGCTCTCGGGACTCGCACTGCGCCTGGTGCGCGACAACCTCATCTCCGAGCG
TGAAGCGATCGCTGCCTACGACGACGCGACCCGCCGCCGCCAATCCTTCGTGTCCTATCTCGTCGAGCAGAAGCTGCTCG
ACGGTCGGAAAATCGCCGTCGCGGCCTCGCACGAGTTCGGCGTGCCGCTCCTCGACCTCCATGCGCTGGATTTGCTGAAC
CTTCCGGTGAATCTGGTCGACGAGCGCCTGGTGCGCAAACACCGGGCGCTGCCGATCTTCCGCCGCGGCAACCGCCTCTT
CCTGGCCTTGTCGGATCCGACCAACGACCAAGCGCTCGAAGAGATCCGGTTCAACACCGGGCTGGCGACCGACGCGGTAC
TGGTTGAGGAGGACAAGCTCAAGGCCGCGATCGAGACCGCGATCGAGGCCCAGGACACGACCATGACGGAGCTCATGGAC
GCGGACCTGGAGAAGCTCGAGATCACCCCGGACGACGAGGATCTGCGCGAAGACGCCGACATCAACATCGACGACGCGCC
GATCGTGCGGTACGTGAACAAGGTCTTGGTCGATGCCATCTCGGCGGGCGCCTCGGACATCCACTTCGAGCCCTACGAGA
AGTTCTTTCGCGTGCGCTTCAGACAAGACGGCATGCTGCGCGAGGTGGCGAGCCCACCCATGAATATCGCCGGGCGATTG
GTCGCACGCCTCAAGGTCATGTCGCGGATGAATATCGCCGAGCGTCGTGTCCCTCAGGACGGACGCATCAAGCTCAAGGT
CAGTCGGACGCGCGACATCGATTTCCGCGTCAATACCTTGCCGACCCTTTACGGCGAGAAGGTGGTCCTGCGGATCCTCG
ATTCCGCGTCGGCTCAGGTCGGCATCGAGGCCCTGGGCTTCGAGCTCGAGCAGCGCGAGGCATTCCTGGAGGCCATCCAT
AAACCCTACGGCATGATCCTGGTGACCGGACCGACCGGATCCGGGAAGACGGTGTCGCTCTACACCGCGCTGAATCTCCT
GAATCAGCCGGACATCAATATCTCGACGGTCGAAGATCCGGTCGAGATCCAGGTTCCGGGAATCAATCAGGTCAGCATGA
ATCCCAAGACGGGGCTGACCTTCGCCGCCGCGCTGCGGGCGTTCCTGCGTCAGGACCCGGACATCGTCATGGTCGGCGAG
ATTCGCGACCTGGAGACCGCGGAGATCGCGGTCAAGGCCGCGCAGACCGGGCATCTGGTGCTCTCCACGCTCCATACCAA
CGACGCCCCGCAGTCGCTGACCCGTCTCGCCAACATGGGCGTGGCACCCTTCAACATTGCCTCCTCCGTCCTGCTCATCA
TGGCGCAGCGTCTCGCGCGGCGCCTCTGCCCGACCTGCAAGGCGCCCGAGGACCTGCCGCGCGAGGTCCTGCGCCAAGAG
GGCTTCAACGAAGCGGATATCGAGGAGGGGATCGTCGTCTTCGGACCGGTGGGATGCGAGCGCTGCACCAAGGGCTACCG
CGGGCGTGTCGGTATCTTCCAAGTGATGCCCATATCGGAGACGATCCGGCGGCTGATTCTCGAGGGAGGCAACGCGATGC
AGCTCGCCGAGCAGGCCCGGCGCGAAGGCGTCGCGGACCTGCGCGAATCCGGTCTGCGCAAGGTCAAAGAAGGGATGACG
AGCCTCGAAGAGCTCAACCGAGTCACCAAGGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

55.712

99.65

0.555
  pilB Acinetobacter baylyi ADP1

54.657

99.65

0.545
  pilB Acinetobacter baumannii D1279779

54.306

99.65

0.541
  pilB Vibrio cholerae strain A1552

49.466

98.424

0.487
  pilF Neisseria gonorrhoeae MS11

46.975

98.424

0.462
  pilB Vibrio parahaemolyticus RIMD 2210633

49.526

92.294

0.457
  pilB Vibrio campbellii strain DS40M4

49.24

92.119

0.454
  pilF Thermus thermophilus HB27

42.176

91.769

0.387
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.967

85.639

0.377