Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KFB96_RS12020 Genome accession   NZ_CP073760
Coordinates   2665382..2666260 (+) Length   292 a.a.
NCBI ID   WP_213461622.1    Uniprot ID   -
Organism   MAG: Thiocapsa sp. isolate M50B4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2660382..2671260
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFB96_RS12005 (KFB96_12005) - 2661378..2662211 (+) 834 WP_213461615.1 NYN domain-containing protein -
  KFB96_RS12010 (KFB96_12010) pilB 2662230..2663945 (+) 1716 WP_213461618.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KFB96_RS12015 (KFB96_12015) pilC 2663957..2665210 (+) 1254 WP_213461620.1 type II secretion system F family protein Machinery gene
  KFB96_RS12020 (KFB96_12020) pilD 2665382..2666260 (+) 879 WP_213461622.1 A24 family peptidase Machinery gene
  KFB96_RS12025 (KFB96_12025) coaE 2666398..2667015 (+) 618 WP_213461624.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 292 a.a.        Molecular weight: 31750.48 Da        Isoelectric Point: 6.5940

>NTDB_id=560538 KFB96_RS12020 WP_213461622.1 2665382..2666260(+) (pilD) [MAG: Thiocapsa sp. isolate M50B4]
MDWIGVFEQTPLLLYAVSILVGLIVGSFLNVVILRLPRMLEDDWARDCAELSGTANPSEPPEQKRLSLAHPPSTCSHCGH
RIRAHENIPVLSYLLLRGRCSACGAGIGLRYPLIEGFTALLTLIVVLQFGLGWQTGPALLLTWALIALAAIDFDTQLLPD
SITLPLIWLGLILSLFTVFTDSHSAIIGAVAGYLSLWSIFHLFRLTTGKEGMGYGDFKLLALLGAWLGWQSLPQIILLSA
LTGAVLGVALILSGRHERGTPMPFGPFLAAAGWISLIWGEQINRTYLQMVGL

Nucleotide


Download         Length: 879 bp        

>NTDB_id=560538 KFB96_RS12020 WP_213461622.1 2665382..2666260(+) (pilD) [MAG: Thiocapsa sp. isolate M50B4]
ATGGATTGGATTGGCGTTTTCGAGCAAACCCCGCTGCTGCTCTATGCGGTCTCGATCCTCGTCGGACTGATCGTCGGCAG
CTTTCTCAATGTCGTCATCCTGCGGCTTCCTCGAATGCTCGAAGACGACTGGGCGCGCGACTGCGCCGAGCTCTCCGGCA
CGGCGAATCCGAGCGAGCCACCTGAGCAAAAACGACTTTCGCTGGCCCATCCGCCCTCCACCTGCTCGCACTGCGGTCAT
CGGATTCGCGCGCACGAGAACATCCCGGTCTTGAGCTATCTCCTCCTGCGCGGTCGATGCTCGGCCTGCGGGGCGGGGAT
CGGTCTGCGCTATCCATTGATCGAGGGCTTCACGGCGCTCCTGACCCTGATCGTCGTCCTGCAGTTCGGTCTCGGCTGGC
AGACCGGCCCCGCCCTCCTGCTCACCTGGGCACTGATCGCGCTTGCCGCGATCGACTTCGATACCCAACTTCTGCCGGAC
AGCATCACACTGCCGTTGATTTGGCTCGGCTTGATCCTGAGCCTCTTCACGGTCTTCACCGACAGTCACTCGGCGATCAT
CGGCGCTGTCGCCGGCTACCTCAGTCTCTGGTCGATCTTCCATCTCTTTCGCCTCACCACCGGGAAGGAAGGCATGGGCT
ACGGCGACTTCAAGCTGCTGGCACTCCTCGGCGCTTGGCTGGGTTGGCAGTCCCTGCCGCAGATCATCCTCTTGTCCGCC
TTGACCGGCGCAGTGCTCGGGGTCGCACTGATCCTGAGCGGCCGCCATGAGCGGGGCACGCCCATGCCGTTCGGTCCCTT
TCTCGCGGCAGCGGGCTGGATCAGCCTGATCTGGGGGGAGCAGATCAACCGGACCTACTTGCAGATGGTGGGGCTCTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(140-248)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-129)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

52.982

97.603

0.517
  pilD Vibrio campbellii strain DS40M4

51.748

97.945

0.507
  pilD Acinetobacter baumannii D1279779

50.523

98.288

0.497
  pilD Acinetobacter nosocomialis M2

50.174

98.288

0.493
  pilD Neisseria gonorrhoeae MS11

47.925

90.753

0.435