Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KEM63_RS01665 Genome accession   NZ_CP073751
Coordinates   364750..366930 (+) Length   726 a.a.
NCBI ID   WP_223654360.1    Uniprot ID   -
Organism   Halopseudomonas nanhaiensis strain SCS 2-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 359750..371930
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KEM63_RS01645 (KEM63_01635) pilM 361904..362968 (+) 1065 WP_223654352.1 pilus assembly protein PilM Machinery gene
  KEM63_RS01650 (KEM63_01640) - 362968..363546 (+) 579 WP_223654354.1 PilN domain-containing protein -
  KEM63_RS01655 (KEM63_01645) pilO 363543..364166 (+) 624 WP_223654356.1 type 4a pilus biogenesis protein PilO -
  KEM63_RS01660 (KEM63_01650) - 364169..364693 (+) 525 WP_223654358.1 pilus assembly protein PilP -
  KEM63_RS01665 (KEM63_01655) pilQ 364750..366930 (+) 2181 WP_223654360.1 type IV pilus secretin PilQ family protein Machinery gene
  KEM63_RS01670 (KEM63_01660) aroK 366936..367451 (+) 516 WP_223654362.1 shikimate kinase AroK -
  KEM63_RS01675 (KEM63_01665) aroB 367533..368612 (+) 1080 WP_223654363.1 3-dehydroquinate synthase -
  KEM63_RS01680 (KEM63_01670) - 368609..370144 (+) 1536 WP_223654364.1 AAA family ATPase -

Sequence

Protein


Download         Length: 726 a.a.        Molecular weight: 78159.27 Da        Isoelectric Point: 4.5630

>NTDB_id=560414 KEM63_RS01665 WP_223654360.1 364750..366930(+) (pilQ) [Halopseudomonas nanhaiensis strain SCS 2-3]
MPLTRKFSLAAVLAVLSPWVAAADLQKLDVASLPGERVELKLTFDEPVATPKGYTIEQPARIALDLPGVKNKLGERGREL
GLGNARSLNIVEANDRTRLIVNLTNLAPYTTRVEGNNLFILVGTDSAPGYSAPATASAGSAASASSNNWRSADQPAVSSA
AASGGKAIRSLDFQRGEQGEGNVIIDLSDPSIRVDVDEQGGRVRLNFPDTRLPDELRVRLDVKDFATPVNFINASTSGGN
TTINIEPSGYFEHLVYQAEDRLIVSFKPLSRGEVEQRRADEFAYTGEKLSLNFQNIEVRSVLQLIADFTDLNLVASDTVE
GSITLRLQNVPWDQALDLVLKTKGLDKRRVGNVLLVAPADEIAARERQELESQRQIAELAPLRRELIQVNYARAADIAKL
FASVTAGAQGEQRGSITVDDRTNSIIALETQDKLDELRRIVTQLDIPVRQVMIEARIVEANVGFDKALGVRWGGNVNLGS
RFNAYGKDGNMGVVEDPNPVTVTDPITGATTEISRNTAGFIGIPEIPLNTPFVDMGVAGATSGIGIGFITNNAILDLQLS
AMESTGNGEIISQPKVVTSDKETAKILKGSEIPYQEASSSGATTTAFAEAVLSLEVTPQITPDNQVIMEVVVTKDEPDFT
NEVNGVPTIRKNEVNAKILVADGETIVIGGVFSSETQQAQEKVPFLGDLPVVGRAFRRDITSESKTELLVFLTPRIINNG
AISVAR

Nucleotide


Download         Length: 2181 bp        

>NTDB_id=560414 KEM63_RS01665 WP_223654360.1 364750..366930(+) (pilQ) [Halopseudomonas nanhaiensis strain SCS 2-3]
ATGCCTCTAACCAGGAAGTTTTCGCTCGCTGCGGTCTTGGCCGTACTCAGCCCGTGGGTGGCCGCCGCCGACCTGCAGAA
ACTGGATGTAGCCTCTCTGCCTGGTGAACGGGTTGAGCTCAAGCTCACATTCGACGAACCGGTCGCTACGCCGAAGGGTT
ACACCATCGAGCAGCCCGCTCGGATCGCGCTGGATCTGCCGGGCGTGAAGAACAAGCTCGGCGAACGCGGCCGGGAGCTA
GGCCTGGGTAACGCGCGCAGCCTGAACATTGTCGAAGCCAACGACCGTACCCGTCTTATCGTCAACCTGACGAATCTCGC
GCCCTATACCACGCGGGTAGAAGGCAACAATCTGTTCATTCTGGTCGGCACCGACAGCGCGCCGGGCTATTCCGCCCCGG
CGACGGCGTCGGCCGGCTCTGCTGCTTCGGCGTCCTCGAACAACTGGCGTTCCGCTGACCAGCCTGCCGTGTCCTCCGCT
GCCGCGTCCGGCGGCAAGGCCATCCGCAGTCTGGATTTCCAGCGCGGAGAGCAGGGTGAAGGCAACGTCATCATCGACCT
GTCGGATCCGTCGATCCGCGTCGACGTGGACGAGCAGGGCGGACGCGTGCGGCTGAATTTCCCGGATACCCGTCTGCCTG
ACGAGTTGCGCGTCCGGCTCGATGTGAAGGACTTCGCCACGCCGGTCAACTTCATCAATGCCAGCACATCCGGCGGCAAT
ACCACGATCAACATCGAGCCCAGCGGCTACTTCGAGCATCTGGTCTACCAGGCTGAAGATCGGCTGATCGTCAGCTTCAA
GCCGCTTAGCCGTGGTGAGGTCGAGCAGCGCCGCGCCGATGAGTTTGCCTACACCGGCGAGAAGCTGTCGTTGAACTTCC
AGAACATCGAAGTGCGCTCAGTGCTGCAGCTGATTGCCGATTTCACCGACCTGAACCTGGTTGCCAGCGACACGGTGGAA
GGCAGCATCACACTGCGTCTGCAGAATGTGCCCTGGGATCAGGCGCTGGACCTGGTTCTGAAAACCAAGGGTCTGGACAA
GCGCCGCGTCGGTAACGTGCTGCTGGTTGCCCCGGCGGACGAAATTGCTGCCCGCGAGCGTCAGGAGCTGGAATCCCAAC
GTCAGATCGCCGAGCTGGCTCCGCTGCGTCGTGAGTTGATCCAGGTGAACTACGCCCGTGCCGCCGACATTGCCAAGCTG
TTCGCGTCGGTCACTGCTGGCGCGCAGGGGGAGCAGCGTGGCTCGATCACCGTCGACGATCGCACCAACAGCATCATCGC
CCTGGAGACCCAGGACAAGCTCGACGAGCTGCGTCGCATCGTGACCCAGCTGGACATTCCGGTCCGTCAGGTGATGATCG
AGGCTCGGATTGTCGAGGCCAACGTCGGCTTCGACAAGGCGCTGGGTGTGCGTTGGGGTGGTAACGTGAACCTGGGCTCT
CGCTTCAACGCGTATGGTAAAGACGGCAACATGGGCGTGGTCGAAGACCCCAACCCGGTTACAGTGACTGACCCGATTAC
CGGTGCCACGACGGAAATATCGCGTAATACCGCTGGCTTTATCGGCATCCCCGAGATTCCGCTGAACACCCCGTTTGTTG
ACATGGGCGTCGCAGGGGCCACGTCCGGTATCGGCATCGGTTTCATCACCAACAATGCGATCCTCGATCTGCAGCTGTCG
GCGATGGAAAGCACCGGTAATGGCGAGATCATTTCCCAGCCCAAGGTCGTGACCTCCGACAAGGAAACCGCGAAGATCCT
CAAGGGCTCCGAGATTCCCTATCAGGAAGCCAGCTCCAGCGGCGCGACCACGACAGCGTTCGCCGAAGCGGTACTGTCGC
TCGAGGTAACGCCACAGATCACCCCTGACAATCAGGTGATCATGGAAGTGGTCGTCACCAAGGACGAGCCGGACTTCACC
AACGAAGTGAACGGCGTGCCGACCATTCGCAAGAACGAGGTCAATGCCAAGATTCTCGTGGCGGACGGCGAAACCATCGT
GATCGGTGGTGTGTTCTCCAGCGAGACCCAGCAGGCGCAGGAGAAGGTGCCCTTCCTGGGTGATCTGCCGGTCGTCGGTC
GGGCTTTCCGTCGGGACATCACGTCCGAGAGCAAGACCGAACTGCTGGTGTTCCTCACGCCCCGCATCATCAACAACGGA
GCGATTTCCGTTGCCAGGTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(312-358)

Bacterial type II/III secretion system short domain (Secretin_N)

(386-450)

Bacterial type II and III secretion system protein (Secretin)

(561-717)

AMIN domain (AMIN)

(39-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

68.457

100

0.685
  comQ Acinetobacter baylyi ADP1

40.479

100

0.419
  pilQ Acinetobacter baumannii strain A118

38.146

100

0.397
  pilQ Acinetobacter baumannii D1279779

38.146

100

0.397
  pilQ Legionella pneumophila strain ERS1305867

38.289

98.209

0.376
  pilQ Legionella pneumophila strain Lp02

38.289

98.209

0.376