Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   KEM63_RS01645 Genome accession   NZ_CP073751
Coordinates   361904..362968 (+) Length   354 a.a.
NCBI ID   WP_223654352.1    Uniprot ID   -
Organism   Halopseudomonas nanhaiensis strain SCS 2-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 356904..367968
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KEM63_RS01635 (KEM63_01625) - 357495..359189 (-) 1695 WP_223654348.1 AMP-binding protein -
  KEM63_RS01640 (KEM63_01630) - 359323..361719 (-) 2397 WP_223654350.1 penicillin-binding protein 1A -
  KEM63_RS01645 (KEM63_01635) pilM 361904..362968 (+) 1065 WP_223654352.1 pilus assembly protein PilM Machinery gene
  KEM63_RS01650 (KEM63_01640) - 362968..363546 (+) 579 WP_223654354.1 PilN domain-containing protein -
  KEM63_RS01655 (KEM63_01645) pilO 363543..364166 (+) 624 WP_223654356.1 type 4a pilus biogenesis protein PilO -
  KEM63_RS01660 (KEM63_01650) - 364169..364693 (+) 525 WP_223654358.1 pilus assembly protein PilP -
  KEM63_RS01665 (KEM63_01655) pilQ 364750..366930 (+) 2181 WP_223654360.1 type IV pilus secretin PilQ family protein Machinery gene
  KEM63_RS01670 (KEM63_01660) aroK 366936..367451 (+) 516 WP_223654362.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38064.67 Da        Isoelectric Point: 4.3800

>NTDB_id=560413 KEM63_RS01645 WP_223654352.1 361904..362968(+) (pilM) [Halopseudomonas nanhaiensis strain SCS 2-3]
MLGLIKKKSNALLGIDISSTTVKLLELSRSGNRYKVEAYAVEPLPPSAVVEKNIVELEGVGQALEKVLARSRTSLKQAAV
AVAGSAVITKIIEMDAGLDDAEMEEQIKLEADQYIPYPLDEVAIDFEVQGPSPRNPERVEVLLAACRRENVDIREAALAL
AGLDVKIVEVEAYAMERACELVVGQLDGGDQAPTIAVIDIGATMMTLSVLSKGRIIYTREQLFGGKQLTDEIQRRYGLSQ
EEAGLAKKQGGLPDDYESEVLSPFKDAVVQQVSRSLQFFFAGGQYNDVDYILLAGGTASIPGLDQLVQQKLGTTTLVANP
FANMTLSSKVNAVALSNDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=560413 KEM63_RS01645 WP_223654352.1 361904..362968(+) (pilM) [Halopseudomonas nanhaiensis strain SCS 2-3]
GTGCTAGGGCTCATAAAGAAGAAGTCGAATGCGCTTCTGGGTATCGACATCAGCTCCACGACCGTGAAGCTGCTGGAACT
CAGTCGCTCGGGTAACCGCTACAAGGTTGAAGCCTACGCGGTTGAACCTCTGCCACCCAGCGCGGTGGTCGAGAAAAACA
TTGTCGAGCTCGAAGGGGTCGGCCAGGCGCTGGAAAAGGTCCTGGCACGCTCGCGAACCTCGCTCAAACAGGCCGCCGTC
GCGGTGGCCGGCTCCGCGGTGATCACCAAGATCATCGAGATGGATGCCGGTCTCGACGATGCCGAGATGGAAGAGCAGAT
CAAGCTCGAGGCCGACCAGTACATTCCCTACCCACTCGACGAGGTCGCCATCGACTTCGAGGTGCAGGGTCCGTCACCGC
GCAATCCTGAACGTGTCGAAGTCCTGCTGGCAGCCTGCCGCCGGGAGAACGTCGACATCCGTGAGGCCGCGCTGGCGCTG
GCCGGGCTCGATGTGAAAATCGTCGAGGTCGAGGCGTACGCCATGGAGCGCGCCTGTGAGCTTGTCGTCGGACAGCTCGA
CGGTGGCGATCAGGCACCGACCATCGCGGTGATCGACATCGGCGCGACGATGATGACGCTCAGTGTCCTGAGCAAGGGTC
GCATCATCTATACCCGCGAGCAGCTGTTCGGTGGCAAGCAGCTAACCGACGAGATTCAGCGCCGCTACGGTCTTTCGCAG
GAAGAGGCGGGTCTGGCCAAGAAGCAGGGCGGTCTGCCTGACGACTACGAAAGCGAAGTCCTCAGCCCGTTCAAGGACGC
GGTCGTGCAGCAGGTCTCACGCTCGCTGCAGTTCTTCTTTGCCGGTGGCCAGTACAACGACGTCGACTACATCCTCCTCG
CTGGCGGCACAGCGTCGATTCCGGGTCTGGATCAGCTGGTTCAGCAGAAGCTCGGTACCACGACGCTGGTCGCCAACCCC
TTCGCGAACATGACCCTGTCCAGCAAGGTGAACGCGGTAGCGCTCAGCAATGACGCGCCGGCGCTGATGATCGCCTGCGG
TCTGGCAATGAGGAGTTTCGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

57.062

100

0.571
  comM Acinetobacter nosocomialis M2

57.062

100

0.571
  comM Acinetobacter baylyi ADP1

54.237

100

0.542
  pilM Legionella pneumophila strain ERS1305867

46.328

100

0.463