Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KC397_RS12595 Genome accession   NZ_CP073273
Coordinates   2676930..2678618 (+) Length   562 a.a.
NCBI ID   WP_212570741.1    Uniprot ID   -
Organism   Vibrio fluvialis strain 19-VB00936     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2671930..2683618
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KC397_RS12565 (KC397_12565) dsbC 2672314..2673078 (-) 765 WP_047459086.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  KC397_RS12570 (KC397_12570) xerD 2673095..2674003 (-) 909 WP_047459088.1 site-specific tyrosine recombinase XerD -
  KC397_RS12575 (KC397_12575) fldB 2674144..2674665 (+) 522 WP_020430452.1 flavodoxin FldB -
  KC397_RS12580 (KC397_12580) ampD 2674670..2675221 (-) 552 WP_020331981.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KC397_RS12585 (KC397_12585) nadC 2675374..2676261 (+) 888 WP_212570737.1 carboxylating nicotinate-nucleotide diphosphorylase -
  KC397_RS12590 (KC397_12590) - 2676492..2676923 (+) 432 WP_212570739.1 pilin -
  KC397_RS12595 (KC397_12595) pilB 2676930..2678618 (+) 1689 WP_212570741.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KC397_RS12600 (KC397_12600) pilC 2678654..2679880 (+) 1227 WP_212570743.1 type II secretion system F family protein Machinery gene
  KC397_RS12605 (KC397_12605) pilD 2679932..2680801 (+) 870 WP_212570745.1 A24 family peptidase Machinery gene
  KC397_RS12610 (KC397_12610) coaE 2680804..2681412 (+) 609 WP_020332197.1 dephospho-CoA kinase -
  KC397_RS12615 (KC397_12615) zapD 2681445..2682185 (+) 741 WP_020332196.1 cell division protein ZapD -
  KC397_RS12620 (KC397_12620) yacG 2682240..2682437 (+) 198 WP_020332195.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62002.91 Da        Isoelectric Point: 6.2299

>NTDB_id=558727 KC397_RS12595 WP_212570741.1 2676930..2678618(+) (pilB) [Vibrio fluvialis strain 19-VB00936]
MLTNLLAILRQADLLSPTQEQAVMDHIHASGTNVPEALIDLAIFHHHELTEHLSQIFGLPNTDLTHFDFAPLCQQLGLRE
LITRYQALPLNSNGQILTLAVSDPTHLQVEDEFRFATGLQIELVLADHKALQAAIRRLYGRAIASHGTQGKEISQDELAS
LVQVSDEEKTSIEDLSQDDSPVSRFINQILLDAVRKGASDIHFEPYESFYRIRLRCDGILVEIQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLNADTAIDMRVSTLPTLWGEKIVLRLLDSSASSLDIDKLGYNDAQKQLYLAALTKPQGMI
LMTGPTGSGKTVSLYTGLRILNTDEVNISTAEDPVEINLPGINQVQVQPKIGFGFAQALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAESVIRLANMGIEPFNLASSLSLIIAQRLARRLCPHCKVATSPSPTLQQQFHLSSQQ
PIYAANPSGCNECTNGYSGRVGIYEVMAFNSALAEAIMQRASIHQIESIAQQHGMQTLLQSGIEKLRAGITSFSELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=558727 KC397_RS12595 WP_212570741.1 2676930..2678618(+) (pilB) [Vibrio fluvialis strain 19-VB00936]
ATGCTCACCAACCTACTCGCCATCCTGCGTCAGGCCGATTTGCTTAGCCCTACGCAGGAACAGGCCGTAATGGATCATAT
TCACGCTTCCGGAACCAATGTTCCGGAAGCGTTGATCGATTTGGCGATCTTCCATCACCATGAACTGACCGAGCATCTAA
GCCAGATCTTTGGTCTGCCGAATACGGATCTGACCCATTTTGACTTCGCGCCGCTGTGCCAGCAGTTAGGGCTGCGAGAG
CTCATCACCCGTTATCAGGCTCTCCCACTCAACAGCAACGGTCAGATACTGACTCTTGCGGTATCCGATCCTACTCACCT
GCAAGTTGAAGACGAGTTTCGTTTTGCGACCGGGTTACAAATAGAACTGGTATTGGCTGATCATAAGGCACTGCAAGCCG
CTATTCGCCGCTTGTATGGCCGAGCCATCGCATCACATGGCACGCAGGGCAAGGAGATCAGCCAGGACGAACTGGCCAGT
CTGGTGCAAGTTTCCGATGAAGAAAAAACATCAATCGAAGATCTGAGTCAGGATGACTCCCCGGTCAGTCGTTTCATCAA
CCAGATCCTGCTGGACGCGGTGCGTAAAGGCGCTTCTGATATCCACTTTGAACCCTATGAATCCTTTTATCGCATTCGCC
TGCGCTGCGACGGTATTTTGGTGGAAATTCAGCAACCAGCCAGCCATTTAAGCCGCCGACTGGCTGCGCGAATTAAGATC
TTATCGAAACTCGATATCGCTGAGCGACGTTTACCGCAAGACGGCCGCATCAAATTACGCCTCAATGCGGACACGGCCAT
TGATATGCGGGTCTCCACTCTGCCAACCTTGTGGGGGGAGAAAATCGTCCTGCGTCTGCTCGACAGCAGTGCGTCCAGCC
TCGATATCGACAAGTTGGGTTATAACGATGCGCAAAAGCAGCTTTATCTGGCGGCCCTCACCAAGCCGCAAGGTATGATT
CTGATGACTGGCCCGACGGGCAGCGGTAAGACAGTCTCACTCTATACCGGCCTTCGCATTCTCAATACCGACGAAGTGAA
TATTTCTACCGCCGAAGACCCGGTGGAAATTAACCTGCCCGGCATTAACCAGGTTCAGGTGCAGCCCAAAATTGGTTTTG
GCTTTGCGCAGGCTCTGCGCTCCTTTCTGCGTCAGGATCCCGATGTGGTGATGGTGGGCGAAATCCGCGATTTGGAAACG
GCAGAGATTGCCGTCAAAGCCGCCCAAACCGGCCACTTAGTGCTATCCACACTACACACCAACTCCGCCGCCGAAAGCGT
GATTCGGCTGGCGAACATGGGCATTGAACCGTTCAACCTCGCTTCATCGCTGAGCCTCATCATCGCTCAACGACTTGCCC
GGCGCCTTTGCCCTCACTGCAAAGTCGCCACATCGCCCTCTCCGACACTGCAGCAACAATTTCACTTGAGTTCTCAGCAG
CCTATCTATGCCGCCAACCCATCAGGCTGCAACGAGTGCACCAACGGTTACAGCGGGCGGGTGGGGATTTATGAAGTGAT
GGCATTTAACTCCGCGCTGGCTGAAGCGATTATGCAACGAGCCAGCATCCATCAAATCGAATCCATTGCCCAGCAACATG
GCATGCAAACGCTGTTGCAATCGGGGATCGAAAAACTTCGCGCCGGGATCACCAGTTTCAGTGAGTTGCAGCGTGTTCTC
TATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

82.384

100

0.824
  pilB Vibrio campbellii strain DS40M4

72.42

100

0.724
  pilB Vibrio parahaemolyticus RIMD 2210633

71.53

100

0.715
  pilB Legionella pneumophila strain ERS1305867

52.224

91.993

0.48
  pilB Acinetobacter baumannii D1279779

50.194

91.815

0.461
  pilB Acinetobacter baylyi ADP1

48.674

93.95

0.457
  pilF Neisseria gonorrhoeae MS11

44.681

100

0.448
  pilF Thermus thermophilus HB27

37.722

100

0.377
  pilB/pilB1 Synechocystis sp. PCC 6803

37.818

97.865

0.37