Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   KCG51_RS02655 Genome accession   NZ_CP073119
Coordinates   533011..534690 (-) Length   559 a.a.
NCBI ID   WP_107810558.1    Uniprot ID   -
Organism   Neisseria subflava strain HP0069     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 528011..539690
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KCG51_RS02625 (KCG51_02590) lspA 528366..528872 (-) 507 WP_049331903.1 signal peptidase II -
  KCG51_RS02630 (KCG51_02595) glyQ 529021..529920 (-) 900 WP_003685919.1 glycine--tRNA ligase subunit alpha -
  KCG51_RS02635 (KCG51_02600) yacG 530002..530184 (-) 183 WP_250579032.1 DNA gyrase inhibitor YacG -
  KCG51_RS02640 (KCG51_02605) coaE 530166..530801 (-) 636 WP_250579033.1 dephospho-CoA kinase -
  KCG51_RS02645 (KCG51_02610) pilD 530801..531688 (-) 888 WP_250579034.1 A24 family peptidase Machinery gene
  KCG51_RS02650 (KCG51_02615) pilG 531690..532931 (-) 1242 WP_254342735.1 type II secretion system F family protein Machinery gene
  KCG51_RS02655 (KCG51_02620) pilF 533011..534690 (-) 1680 WP_107810558.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KCG51_RS02660 (KCG51_02625) - 535083..536144 (-) 1062 WP_254342737.1 nucleotidyltransferase domain-containing protein -
  KCG51_RS02665 (KCG51_02630) - 536159..536917 (-) 759 WP_254342739.1 nucleotidyltransferase domain-containing protein -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62236.46 Da        Isoelectric Point: 6.0382

>NTDB_id=558151 KCG51_RS02655 WP_107810558.1 533011..534690(-) (pilF) [Neisseria subflava strain HP0069]
MSVGLLRVLVQSQTISNQQAEHYNSLLQSGKEILPNLFSDGIISPKALGELVARVFSYPLLDLHYYPRNNVVSDILTEEQ
MVQNRCIPIFKRGRKVYFAVSDPTQIQNFQKIAFASGLSVDLVVVPDDQLSSLLEWLGQRSTTILKEINDEHEATQQSQS
LYIDNEEAEDGPIPRFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISEK
RIPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDGTSLNIDQLGLEPFQKEMLLEAINRPYGMVLVTGPTGS
GKTVSLYTCLSILNTEDVNISTAEDPAEINLPGVNQVNVNEKQGLTFAAALRSFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRLLNMGVAPFNIASSVSLIMAQRLLRRLCPNCKREVERPPVPALKKAGFTDADLAQDWKL
YRPVGCDSCRGKGFKGRVGIYEVMPVSDEMQKVIMNNGTEVDIMNMAYQEGMVDLRRAGLMKVMQGLTSLEEVTAHTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=558151 KCG51_RS02655 WP_107810558.1 533011..534690(-) (pilF) [Neisseria subflava strain HP0069]
ATGAGTGTCGGATTATTGCGTGTTTTGGTTCAAAGCCAGACCATCTCAAATCAACAAGCGGAACATTACAACAGCCTTTT
ACAATCGGGCAAAGAAATCCTGCCCAACCTGTTTTCAGACGGCATCATCTCCCCTAAAGCTTTGGGAGAGTTAGTCGCGC
GCGTATTCAGCTACCCTCTTTTAGATTTGCACTACTACCCGCGCAATAATGTAGTCAGCGACATTCTGACGGAAGAGCAA
ATGGTGCAAAACCGTTGCATTCCGATTTTCAAACGCGGACGCAAGGTTTATTTCGCCGTATCCGATCCGACCCAAATCCA
AAACTTTCAAAAAATCGCTTTTGCTTCAGGTTTAAGTGTCGACTTGGTCGTGGTTCCAGACGACCAGCTCAGCTCATTGC
TGGAATGGCTGGGTCAACGTTCGACCACCATTCTGAAAGAAATCAACGACGAACATGAAGCAACCCAGCAATCTCAATCG
CTTTATATCGACAATGAAGAAGCTGAAGACGGCCCTATCCCCCGTTTCATCCACAAAACCTTATCCGATGCCCTGAACGC
GGGAGCATCAGACATCCACTTTGAATTCTACGAGCAAATGGCACGCGTCCGTTTCCGTGTGGACGGGCAGTTACGTGAAG
TCGTACAGCCGCCTGTCGCCGTGCGCGGCCAGTTGGCATCGCGCATCAAAGTGATGGCGCGTTTGGATATTTCCGAAAAA
CGTATTCCGCAAGACGGCAGAATTCAAATTGCCTTCCATAAACACGGTCGTCCGATTGACTTCCGTGTCAGCACTTTGCC
GACATTGTTTGGTGAAAAAGTGGTAATGCGTATCCTAAATTCCGATGGTACATCACTCAATATCGACCAGCTCGGTTTAG
AGCCTTTCCAAAAAGAAATGCTGCTTGAGGCCATCAACCGTCCTTACGGCATGGTGTTGGTTACCGGTCCGACAGGCTCG
GGCAAAACCGTTTCGCTCTATACCTGCCTGAGTATTTTGAACACGGAAGATGTCAACATTTCAACGGCAGAAGATCCGGC
CGAGATTAACCTGCCAGGCGTCAACCAAGTCAACGTCAATGAAAAACAAGGCCTGACGTTTGCTGCCGCACTCCGATCCT
TCTTGCGTCAAGATCCTGATATTATTATGGTCGGTGAGATTCGTGACTTGGAAACCGCCGACATCGCCATTAAAGCCGCC
CAAACCGGCCACATGGTTTTCTCGACCCTGCATACCAATAATGCACCGGCCACGCTGTCCCGCCTTTTGAACATGGGCGT
AGCGCCTTTCAATATCGCCAGCTCGGTCAGCCTAATTATGGCGCAAAGGCTGTTGCGCAGACTGTGTCCGAACTGCAAAC
GCGAAGTTGAGCGCCCACCGGTTCCGGCATTGAAAAAAGCCGGCTTTACCGATGCGGACTTGGCTCAAGATTGGAAGCTC
TACCGCCCGGTTGGCTGCGACAGCTGCCGCGGTAAAGGATTTAAAGGCCGCGTCGGCATTTATGAAGTCATGCCGGTCAG
CGATGAAATGCAAAAAGTCATTATGAACAACGGTACAGAAGTTGATATCATGAACATGGCCTATCAGGAAGGCATGGTAG
ACTTGCGTCGTGCCGGCCTCATGAAAGTCATGCAAGGCCTGACCTCGTTGGAAGAAGTGACTGCCCATACCAATGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)

Type II/IV secretion system protein (T2SSE)

(179-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

77.46

100

0.775
  pilB Acinetobacter baylyi ADP1

51.056

100

0.519
  pilB Acinetobacter baumannii D1279779

50.442

100

0.51
  pilB Legionella pneumophila strain ERS1305867

47.173

100

0.478
  pilB Vibrio cholerae strain A1552

46.085

100

0.463
  pilB Vibrio campbellii strain DS40M4

44.602

100

0.451
  pilB Vibrio parahaemolyticus RIMD 2210633

46.542

95.707

0.445
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.364

98.39

0.377
  pilF Thermus thermophilus HB27

38.547

96.064

0.37