Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   KCG55_RS05775 Genome accession   NZ_CP073117
Coordinates   1175076..1176755 (-) Length   559 a.a.
NCBI ID   WP_254322304.1    Uniprot ID   -
Organism   Neisseria subflava strain HP0015     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1170076..1181755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KCG55_RS05745 (KCG55_05750) lspA 1170432..1170938 (-) 507 WP_254322297.1 signal peptidase II -
  KCG55_RS05750 (KCG55_05755) glyQ 1171087..1171986 (-) 900 WP_070824173.1 glycine--tRNA ligase subunit alpha -
  KCG55_RS05755 (KCG55_05760) yacG 1172068..1172250 (-) 183 WP_003682475.1 DNA gyrase inhibitor YacG -
  KCG55_RS05760 (KCG55_05765) coaE 1172247..1172867 (-) 621 WP_254322299.1 dephospho-CoA kinase -
  KCG55_RS05765 (KCG55_05770) pilD 1172867..1173754 (-) 888 WP_254322300.1 A24 family peptidase Machinery gene
  KCG55_RS05770 (KCG55_05775) pilG 1173756..1174997 (-) 1242 WP_254322302.1 type II secretion system F family protein Machinery gene
  KCG55_RS05775 (KCG55_05780) pilF 1175076..1176755 (-) 1680 WP_254322304.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KCG55_RS05780 (KCG55_05785) carB 1177159..1180374 (-) 3216 WP_063069315.1 carbamoyl-phosphate synthase large subunit -
  KCG55_RS05785 (KCG55_05790) - 1180385..1180945 (-) 561 WP_254322306.1 hypothetical protein -
  KCG55_RS05790 (KCG55_05795) - 1181005..1181235 (+) 231 WP_254322307.1 hypothetical protein -
  KCG55_RS05795 (KCG55_05800) - 1181244..1181501 (-) 258 WP_049225703.1 SemiSWEET family transporter -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62258.55 Da        Isoelectric Point: 6.4264

>NTDB_id=558121 KCG55_RS05775 WP_254322304.1 1175076..1176755(-) (pilF) [Neisseria subflava strain HP0015]
MSVGLLRVLVQSQTISNQQAEHYNSLLQSGKEILPNLFSDGIISPKALGELVARVFSYPLLDLRYYPRNNVVSDILTEEQ
MVQNRCIPIFKRGRKVYFAVSDPTQIQNFQKIAFASGLSVDLVVVPDDQLSSLLEWLGQRSTTILKEINDEHEATQQSQS
LYIDNEEAEDGPIPRFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISEK
RIPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDGTSLNIDQLGLETFQKKMLLEAINRPYGMVLVTGPTGS
GKTVSLYTCLSILNTEDVNISTAEDPAEINLPGVNQVNVNEKQGLTFAAALRSFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRLLNMGVAPFNIASSVSLIMAQRLLRRLCPNCKREVERPPVPALKKAGFTDADLAQDWKL
YRPVGCDSCRGKGFKGRVGIYEVMPVSDEMQKVIMNNGTEVDIMNMAYQEGMVDLRRAGLMKVMQGLTSLEEVTAHTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=558121 KCG55_RS05775 WP_254322304.1 1175076..1176755(-) (pilF) [Neisseria subflava strain HP0015]
ATGAGTGTCGGATTATTGCGTGTTTTGGTTCAAAGCCAGACCATCTCAAATCAACAAGCGGAACATTACAACAGCCTTTT
ACAATCAGGCAAAGAAATCCTGCCCAACCTGTTTTCAGACGGCATCATCTCCCCTAAAGCTTTGGGAGAGTTAGTCGCGC
GCGTATTCAGTTATCCCCTTTTGGATTTGCGCTACTACCCGCGCAATAATGTAGTCAGCGACATTCTGACGGAAGAGCAA
ATGGTGCAAAACCGTTGCATTCCGATTTTCAAACGCGGACGCAAGGTTTATTTCGCCGTATCCGATCCGACCCAAATCCA
AAACTTCCAAAAAATCGCTTTTGCTTCAGGTTTAAGTGTCGACTTGGTAGTGGTTCCCGACGACCAGCTCAGCTCATTGC
TGGAATGGCTGGGTCAACGCTCAACCACCATTCTGAAAGAAATCAACGACGAACATGAAGCAACCCAGCAATCGCAATCG
CTTTATATCGATAACGAAGAAGCTGAAGACGGCCCTATCCCCCGTTTCATCCACAAAACCTTATCCGATGCCCTGAACGC
GGGTGCATCGGATATCCACTTTGAATTCTACGAACAAATGGCGCGCGTTCGTTTCCGTGTGGACGGACAATTGCGCGAAG
TCGTACAACCTCCTGTCGCAGTGCGCGGCCAGTTGGCATCGCGTATCAAAGTGATGGCGCGTTTGGATATTTCCGAAAAA
CGTATTCCGCAAGACGGCAGAATTCAAATTGCCTTCCATAAACACGGCCGTCCGATTGACTTCCGTGTCAGCACTTTGCC
GACATTGTTTGGCGAAAAAGTGGTGATGCGTATCCTAAATTCCGATGGTACATCGCTCAATATCGACCAGCTCGGTTTAG
AGACTTTCCAAAAAAAAATGCTGTTGGAAGCCATCAACCGCCCTTACGGCATGGTATTGGTTACCGGTCCGACAGGTTCG
GGCAAAACCGTTTCGCTCTATACCTGCCTGAGTATTTTAAACACGGAAGATGTGAACATTTCGACGGCAGAAGATCCGGC
CGAGATTAACCTACCGGGCGTCAACCAAGTCAACGTCAATGAGAAACAAGGTCTGACGTTTGCCGCCGCACTCCGGTCCT
TCTTACGTCAAGATCCTGATATTATTATGGTCGGTGAGATTCGTGACTTGGAAACCGCCGATATCGCCATTAAAGCCGCC
CAAACCGGCCACATGGTTTTCTCGACCCTGCATACCAATAATGCACCGGCCACGCTGTCCCGCCTTTTGAACATGGGTGT
AGCGCCTTTCAATATCGCCAGCTCGGTCAGCCTGATTATGGCGCAAAGGCTGTTGCGCAGACTGTGTCCAAACTGCAAAC
GCGAAGTTGAACGCCCGCCGGTTCCGGCATTGAAAAAAGCTGGCTTTACCGATGCGGACTTGGCTCAAGATTGGAAACTC
TACCGCCCGGTTGGTTGCGACAGCTGCCGCGGCAAAGGCTTTAAAGGCCGCGTCGGTATTTATGAAGTCATGCCGGTCAG
CGATGAAATGCAAAAAGTCATTATGAACAACGGTACAGAAGTTGATATCATGAACATGGCCTATCAGGAAGGCATGGTAG
ACTTGCGTCGTGCCGGCCTCATGAAAGTCATGCAAGGCCTGACCTCGTTGGAAGAAGTAACTGCTCATACCAATGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)

Type II/IV secretion system protein (T2SSE)

(179-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

77.639

100

0.776
  pilB Acinetobacter baylyi ADP1

50.88

100

0.517
  pilB Acinetobacter baumannii D1279779

50.442

100

0.51
  pilB Legionella pneumophila strain ERS1305867

46.996

100

0.476
  pilB Vibrio cholerae strain A1552

45.907

100

0.462
  pilB Vibrio campbellii strain DS40M4

44.602

100

0.451
  pilB Vibrio parahaemolyticus RIMD 2210633

46.542

95.707

0.445
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.364

98.39

0.377
  pilF Thermus thermophilus HB27

38.175

96.064

0.367