Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   KCG56_RS11270 Genome accession   NZ_CP073116
Coordinates   2338469..2340148 (-) Length   559 a.a.
NCBI ID   WP_070824176.1    Uniprot ID   A0A9X9HYH5
Organism   Neisseria subflava strain TT0073     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2333469..2345148
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KCG56_RS11240 (KCG56_11270) lspA 2333825..2334331 (-) 507 WP_254321467.1 signal peptidase II -
  KCG56_RS11245 (KCG56_11275) glyQ 2334480..2335379 (-) 900 WP_003685919.1 glycine--tRNA ligase subunit alpha -
  KCG56_RS11250 (KCG56_11280) yacG 2335461..2335643 (-) 183 WP_003682475.1 DNA gyrase inhibitor YacG -
  KCG56_RS11255 (KCG56_11285) coaE 2335640..2336260 (-) 621 WP_254321468.1 dephospho-CoA kinase -
  KCG56_RS11260 (KCG56_11290) pilD 2336260..2337147 (-) 888 WP_254321469.1 A24 family peptidase Machinery gene
  KCG56_RS11265 (KCG56_11295) pilG 2337149..2338390 (-) 1242 WP_254321470.1 type II secretion system F family protein Machinery gene
  KCG56_RS11270 (KCG56_11300) pilF 2338469..2340148 (-) 1680 WP_070824176.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KCG56_RS11275 (KCG56_11305) carB 2340553..2343768 (-) 3216 WP_070606039.1 carbamoyl-phosphate synthase large subunit -
  KCG56_RS11280 (KCG56_11310) - 2343800..2344495 (-) 696 WP_254321471.1 hypothetical protein -
  KCG56_RS11285 (KCG56_11315) - 2344596..2344892 (-) 297 WP_254321472.1 hypothetical protein -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62255.51 Da        Isoelectric Point: 6.0846

>NTDB_id=558096 KCG56_RS11270 WP_070824176.1 2338469..2340148(-) (pilF) [Neisseria subflava strain TT0073]
MSVGLLRVLVQSQTISNQQAEHYNSLLQSGKEILPNLFSDGIISPKALGELVARVFSYPLLDLRYYPRNNVVSDILTEEQ
MVQNRCIPIFKRGRKVYFAVSDPTQIQNFQKIAFASGLSVDLVVVPDDQLSSLLEWLGQRSTTILKEINDEHEATQQSQS
LYIDNEEAEDGPIPRFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISEK
RIPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDGTSLNIDQLGLEPFQKEMLLEAINRPYGMVLVTGPTGS
GKTVSLYTCLSILNTEDVNISTAEDPAEINLPGVNQVNVNEKQGLTFAAALRSFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRLLNMGVAPFNIASSVSLIMAQRLLRRLCPNCKREVERPPVPALKKAGFTDADLAQDWKL
YRPVGCDSCRGKGFKGRVGIYEVMPVSDEMQKVIMNNGTEVDIMNMAYQEGMVDLRRAGLMKVMQGLTSLEEVTAHTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=558096 KCG56_RS11270 WP_070824176.1 2338469..2340148(-) (pilF) [Neisseria subflava strain TT0073]
ATGAGTGTCGGATTATTGCGTGTTTTGGTTCAAAGCCAGACCATCTCAAATCAACAAGCGGAACATTACAATAGCCTTTT
ACAATCGGGCAAAGAAATCCTGCCCAACCTGTTTTCAGACGGCATCATCTCCCCTAAAGCTTTGGGAGAGTTGGTCGCGC
GCGTATTCAGCTATCCCCTTTTGGATTTGCGCTACTACCCGCGCAATAATGTAGTCAGCGACATTCTGACGGAAGAGCAA
ATGGTGCAAAACCGTTGCATTCCGATTTTCAAACGCGGACGCAAGGTTTATTTCGCCGTATCCGATCCGACCCAAATCCA
AAACTTCCAAAAAATCGCTTTTGCTTCAGGTTTAAGTGTCGACTTGGTAGTGGTTCCCGACGACCAGCTCAGCTCATTGC
TGGAATGGCTGGGTCAACGTTCGACCACCATTCTGAAAGAAATCAACGACGAACATGAAGCAACGCAGCAATCGCAATCG
CTTTATATCGATAACGAAGAAGCTGAAGACGGCCCTATCCCCCGTTTCATCCACAAAACCTTATCCGATGCCCTGAACGC
GGGCGCATCGGATATCCACTTTGAATTCTACGAGCAAATGGCACGCGTTCGTTTCCGCGTGGATGGACAATTGCGTGAAG
TCGTACAGCCGCCTGTCGCCGTGCGCGGCCAGTTGGCATCGCGTATCAAAGTGATGGCGCGTTTGGATATTTCCGAAAAA
CGTATTCCGCAAGACGGCAGAATTCAAATTGCCTTCCATAAACACGGCCGTCCGATTGACTTCCGTGTCAGCACTTTGCC
GACATTGTTTGGCGAAAAAGTGGTGATGCGTATCCTAAATTCCGATGGTACATCACTCAATATCGACCAGCTCGGCTTAG
AGCCTTTCCAAAAAGAAATGCTGTTGGAAGCCATCAACCGCCCTTACGGCATGGTATTGGTTACCGGTCCGACAGGTTCG
GGCAAAACCGTTTCGCTCTATACCTGCCTGAGTATTTTGAACACGGAAGATGTGAACATTTCGACGGCAGAAGATCCGGC
CGAGATTAATCTGCCGGGCGTCAACCAAGTCAACGTCAATGAGAAACAAGGCCTGACGTTTGCCGCTGCACTCCGATCCT
TCTTGCGTCAAGATCCTGATATTATTATGGTCGGTGAGATTCGTGACTTAGAAACCGCCGATATCGCCATCAAAGCCGCC
CAAACCGGCCACATGGTTTTCTCGACCCTGCATACCAATAATGCACCGGCCACGCTGTCCCGCCTTTTGAACATGGGTGT
AGCGCCTTTCAATATCGCCAGCTCGGTCAGCCTGATTATGGCGCAAAGGCTGTTGCGCAGACTGTGTCCAAACTGCAAAC
GCGAAGTTGAGCGCCCGCCGGTTCCGGCATTGAAAAAAGCCGGCTTTACCGATGCGGACTTGGCTCAAGATTGGAAACTC
TACCGCCCGGTTGGCTGCGACAGCTGCCGCGGCAAAGGCTTTAAAGGCCGCGTCGGTATTTATGAAGTCATGCCGGTCAG
CGATGAAATGCAAAAAGTCATTATGAACAACGGTACAGAAGTTGATATCATGAACATGGCCTATCAGGAAGGCATGGTAG
ACTTGCGTCGCGCCGGCCTCATGAAAGTCATGCAAGGCCTGACCTCGTTGGAAGAAGTAACTGCCCATACCAATGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)

Type II/IV secretion system protein (T2SSE)

(179-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

77.639

100

0.776
  pilB Acinetobacter baylyi ADP1

51.056

100

0.519
  pilB Acinetobacter baumannii D1279779

50.442

100

0.51
  pilB Legionella pneumophila strain ERS1305867

47.173

100

0.478
  pilB Vibrio cholerae strain A1552

46.085

100

0.463
  pilB Vibrio campbellii strain DS40M4

44.602

100

0.451
  pilB Vibrio parahaemolyticus RIMD 2210633

46.542

95.707

0.445
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.364

98.39

0.377
  pilF Thermus thermophilus HB27

38.547

96.064

0.37