Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   J9253_RS19135 Genome accession   NZ_CP072801
Coordinates   3953903..3955624 (-) Length   573 a.a.
NCBI ID   WP_210222437.1    Uniprot ID   -
Organism   Thiothrix litoralis strain AS     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3948903..3960624
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J9253_RS19110 (J9253_19110) opgC 3949260..3950447 (+) 1188 WP_210222432.1 OpgC domain-containing protein -
  J9253_RS19115 (J9253_19115) ubiE 3950393..3951145 (-) 753 WP_210222433.1 bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE -
  J9253_RS19120 (J9253_19120) - 3951220..3951642 (+) 423 WP_210222434.1 hypothetical protein -
  J9253_RS19125 (J9253_19125) pilD 3951655..3952527 (-) 873 WP_210222435.1 A24 family peptidase Machinery gene
  J9253_RS19130 (J9253_19130) pilC 3952536..3953768 (-) 1233 WP_228291435.1 type II secretion system F family protein Machinery gene
  J9253_RS19135 (J9253_19135) pilB 3953903..3955624 (-) 1722 WP_210222437.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J9253_RS19140 (J9253_19140) - 3955716..3957257 (-) 1542 WP_210222438.1 ATP-binding protein -
  J9253_RS19145 (J9253_19145) - 3957347..3958579 (+) 1233 WP_210222439.1 ATP-binding protein -
  J9253_RS19150 (J9253_19150) - 3958674..3959522 (+) 849 WP_210222440.1 Rpn family recombination-promoting nuclease/putative transposase -
  J9253_RS19155 (J9253_19155) - 3959527..3959682 (+) 156 WP_210222441.1 hypothetical protein -
  J9253_RS19160 (J9253_19160) tnpA 3959776..3960036 (+) 261 WP_210222442.1 IS200/IS605 family transposase -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 63063.90 Da        Isoelectric Point: 6.8461

>NTDB_id=555937 J9253_RS19135 WP_210222437.1 3953903..3955624(-) (pilB) [Thiothrix litoralis strain AS]
MIPMTSSIPLPGLARQLVVQDLLDKELAAQAVQKARVEKVSLVEWLIRSRVVSTQDVAYAASLEYGLSMVDLDQVNITSL
VNTLLSQDQMRKLNLIPLFQRGKNLIVGLADPTYLTNLDDVKFATGLIPEPVFVEFDKLHRFLNVDDGMMGSANAPTSGK
TSSSAANAPAAKEISLDVRDLKTKDDEDPAIIEFVDKLLAHGIKSKASDIHIEVFEKNMRIRYRIDGILQVVATPPNDSA
QQLISRIKVLSRMNISERRVPQDGRIRFTVSDQKKIDFRVNTLPTLFGEKVVMRILDSSNATLGVEKLGFDPKQKHDFMK
ALEKPDGMILVTGPTGSGKTVTLYTGLGILNTPEKNISTAEDPAEINMPGVNQVNVNAKVGLTFAEALRSFLRQDPDIIM
VGEIRDLETAEISIKAAQTGHLVLSTLHTNSAPETLTRLMNMGVPPFNIASSVHLIIAQRLARRLCEKCKKTSDVPNEVL
LQMGFKSEELSNLKLYEPVGCSECSRGYKGRVGIYQVMPISGDMGRMIMNGSNSMEIAEQALKEGILDLRQSALNKVRQG
ITSLAELERVTSD

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=555937 J9253_RS19135 WP_210222437.1 3953903..3955624(-) (pilB) [Thiothrix litoralis strain AS]
ATGATTCCCATGACATCCTCCATCCCCTTGCCGGGGCTAGCCAGACAATTGGTCGTGCAAGACTTGCTCGACAAGGAACT
GGCTGCCCAAGCTGTGCAAAAAGCACGGGTAGAAAAAGTGTCGCTGGTCGAATGGCTCATCCGTTCCCGCGTGGTTTCCA
CTCAAGACGTTGCTTATGCCGCTAGCTTGGAATATGGCTTAAGCATGGTCGATCTCGATCAGGTCAATATCACATCCCTA
GTCAACACCCTGCTAAGTCAGGATCAGATGCGCAAACTCAATCTGATCCCCCTGTTCCAACGCGGCAAAAATCTGATCGT
GGGTCTGGCAGACCCCACCTACCTGACCAATCTGGATGATGTTAAATTCGCCACAGGGCTGATTCCCGAACCTGTGTTTG
TGGAATTCGACAAACTGCACCGCTTCCTAAACGTTGATGATGGCATGATGGGGAGCGCCAACGCCCCCACCTCCGGCAAA
ACATCATCCTCAGCGGCTAACGCTCCTGCTGCCAAAGAAATTTCCCTCGATGTCCGCGACCTTAAAACCAAGGACGATGA
AGACCCCGCCATCATTGAATTCGTCGACAAGCTGTTGGCACACGGTATCAAATCCAAAGCCTCGGACATCCACATCGAAG
TTTTTGAAAAAAACATGCGCATCCGCTACCGGATAGACGGCATCCTGCAAGTTGTCGCTACCCCGCCCAACGACTCCGCG
CAACAACTGATTTCACGTATTAAGGTACTGTCGCGAATGAATATTTCCGAACGGCGCGTGCCGCAAGACGGGCGTATCCG
CTTCACCGTCAGTGACCAGAAAAAGATCGACTTCCGGGTCAACACACTGCCCACTCTCTTCGGCGAAAAGGTGGTCATGC
GGATACTCGACTCCTCCAACGCCACGCTAGGCGTGGAAAAGCTGGGGTTCGACCCTAAACAAAAACACGATTTCATGAAG
GCGCTGGAAAAACCCGACGGCATGATTCTGGTAACAGGCCCGACCGGTAGTGGTAAAACCGTCACCCTGTATACCGGCCT
CGGCATCCTCAATACCCCGGAAAAAAATATTTCTACCGCCGAAGACCCGGCTGAAATCAATATGCCCGGCGTTAACCAAG
TCAACGTCAACGCCAAAGTCGGCCTGACCTTTGCCGAAGCCTTGCGCTCCTTCCTGCGTCAAGACCCAGACATTATCATG
GTAGGGGAAATCCGTGACCTGGAAACGGCTGAAATTTCCATAAAGGCGGCACAAACCGGGCACTTGGTATTATCCACCCT
CCACACCAACAGTGCCCCGGAAACCCTCACCCGTTTGATGAACATGGGCGTACCACCCTTCAATATCGCCTCTTCGGTAC
ACCTGATCATTGCCCAACGCCTTGCCCGACGCTTGTGTGAGAAATGCAAAAAAACATCTGATGTACCCAACGAGGTCTTA
TTGCAAATGGGCTTCAAGTCTGAAGAATTATCCAACCTAAAATTGTATGAACCCGTCGGTTGCTCGGAATGTTCACGCGG
TTACAAAGGCCGGGTGGGGATTTATCAAGTCATGCCAATTTCAGGGGATATGGGGCGCATGATCATGAACGGCAGCAACT
CGATGGAAATCGCCGAACAAGCACTCAAAGAAGGCATTCTGGACTTGCGCCAATCGGCCTTGAATAAAGTACGCCAAGGC
ATTACCTCGCTGGCAGAACTGGAGCGTGTCACCTCAGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-144)

Type II/IV secretion system protein (T2SSE)

(198-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

51.37

100

0.524
  pilB Acinetobacter baumannii D1279779

50.957

100

0.511
  pilB Legionella pneumophila strain ERS1305867

50.346

100

0.508
  pilF Neisseria gonorrhoeae MS11

48.587

98.778

0.48
  pilB Vibrio cholerae strain A1552

46.771

100

0.468
  pilB Vibrio campbellii strain DS40M4

46.789

95.113

0.445
  pilB Vibrio parahaemolyticus RIMD 2210633

46.52

95.288

0.443
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.011

95.288

0.372
  pilB/pilB1 Synechocystis sp. PCC 6803

37.297

96.859

0.361
  pilF Thermus thermophilus HB27

37.636

95.986

0.361