Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   J9260_RS17510 Genome accession   NZ_CP072793
Coordinates   3512795..3514504 (-) Length   569 a.a.
NCBI ID   WP_210218987.1    Uniprot ID   -
Organism   Thiothrix unzii strain A1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3507795..3519504
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J9260_RS17485 (J9260_17480) ubiE 3508884..3509636 (-) 753 WP_210218982.1 bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE -
  J9260_RS17490 (J9260_17485) - 3509713..3510132 (+) 420 WP_210218983.1 hypothetical protein -
  J9260_RS17495 (J9260_17490) - 3510230..3510529 (+) 300 WP_210218984.1 hypothetical protein -
  J9260_RS17500 (J9260_17495) pilD 3510551..3511426 (-) 876 WP_210218985.1 A24 family peptidase Machinery gene
  J9260_RS17505 (J9260_17500) pilC 3511446..3512705 (-) 1260 WP_210218986.1 type II secretion system F family protein Machinery gene
  J9260_RS17510 (J9260_17505) pilB 3512795..3514504 (-) 1710 WP_210218987.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J9260_RS17515 (J9260_17510) tviB 3514611..3515888 (-) 1278 WP_210218988.1 Vi polysaccharide biosynthesis UDP-N-acetylglucosamine C-6 dehydrogenase TviB -
  J9260_RS17520 (J9260_17515) - 3516025..3516960 (+) 936 WP_210218989.1 glycosyltransferase family 4 protein -
  J9260_RS17525 (J9260_17520) - 3516974..3517660 (+) 687 WP_210218990.1 class I SAM-dependent methyltransferase -
  J9260_RS17530 (J9260_17525) - 3517635..3517874 (-) 240 WP_210218991.1 hypothetical protein -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62469.04 Da        Isoelectric Point: 6.5108

>NTDB_id=555893 J9260_RS17510 WP_210218987.1 3512795..3514504(-) (pilB) [Thiothrix unzii strain A1]
MIPLNASIPLPGLLRQLVAQEILSNEQASQALQKARIDKMPLVSWLARSRLASTADIAYAASVEYGLSFVDIDQVNITPA
IANLLNAEQMQKLHLIPLYRGAKTLMVGLADPAYLTHLDDVKFATGLTPEPVFVEYDKLQRYLNADSGEITQPNPVVAAQ
QYNSAPSIVLDTRDLKGKNDNEESSVVEFVDKLLAHAINAKASDIHIEIFEKSLRIRYRIDGILQVVATPPNDSAQQLIS
RIKVLARMDISERRVPQDGRIRFTLNAKKKIDFRVNTLPTLYGEKVVMRILDSSNATLGVEKLGFSPRQQQDFLKALQKP
DGMILVTGPTGSGKTVTLYTGLGILNTPERNISTAEDPAEINMPGVNQVNVNAKVGLTFADALRSFLRQDPDVIMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLLNMGVPPFNIASSVHLIVAQRLARRLCEKCKRPATNIPPEALLDLG
FKAEEIPSLTVYEPCGCEECSHGYKGRVGLYQVMPISAEMGRMVMNGCNSMELADQSLKEGIFDLRQSGLEKVRLGITSL
AELSRVTTD

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=555893 J9260_RS17510 WP_210218987.1 3512795..3514504(-) (pilB) [Thiothrix unzii strain A1]
ATGATTCCTCTCAATGCATCGATTCCATTACCCGGTTTGCTACGGCAGTTGGTTGCTCAGGAAATTCTCAGCAATGAGCA
AGCCAGTCAAGCCCTGCAAAAAGCACGGATTGATAAAATGCCGCTGGTGTCCTGGTTGGCAAGGTCACGCCTTGCCAGCA
CAGCGGATATTGCTTACGCAGCAAGTGTGGAATACGGCCTAAGTTTTGTTGATATTGACCAAGTAAACATCACCCCGGCT
ATCGCCAACTTGTTGAATGCCGAACAAATGCAAAAATTGCATCTGATTCCGCTGTATCGTGGTGCAAAAACACTGATGGT
CGGGCTGGCAGACCCGGCATACCTCACGCACTTGGATGACGTGAAATTTGCGACCGGGCTAACCCCCGAACCGGTTTTTG
TCGAATACGATAAATTACAACGTTATTTAAATGCCGACAGCGGCGAAATCACCCAACCCAACCCGGTAGTCGCTGCGCAG
CAATACAATTCCGCACCGTCGATTGTGCTGGACACGCGCGACCTCAAAGGTAAAAACGACAACGAAGAATCTTCCGTCGT
TGAGTTTGTCGATAAGCTGCTGGCACACGCGATCAACGCCAAAGCCTCCGATATTCATATTGAAATTTTTGAGAAAAGCC
TGCGCATCCGCTACCGCATTGACGGTATTTTGCAGGTTGTGGCAACCCCACCGAACGATTCCGCCCAACAATTGATTTCA
CGGATCAAGGTATTAGCGCGTATGGACATTTCCGAGCGGCGTGTACCACAAGACGGGCGCATTCGCTTTACCCTCAATGC
CAAGAAAAAAATCGACTTCCGGGTCAACACCCTGCCCACACTTTACGGCGAAAAAGTGGTCATGCGGATTCTTGATTCCT
CCAACGCCACCCTCGGCGTGGAAAAGTTAGGGTTTAGCCCACGCCAACAACAGGATTTCCTCAAAGCCTTGCAAAAGCCG
GATGGGATGATTTTGGTCACGGGGCCAACGGGAAGTGGTAAAACAGTAACCCTGTACACTGGGCTTGGTATTCTCAATAC
CCCTGAGCGTAATATTTCCACTGCCGAAGACCCCGCTGAAATCAATATGCCCGGTGTTAACCAAGTCAACGTCAACGCCA
AAGTCGGGTTAACTTTTGCGGACGCACTGCGCTCATTCTTGCGCCAAGACCCGGATGTAATCATGGTCGGAGAAATCCGT
GACCTTGAAACCGCTGAAATCGCCATCAAAGCCGCGCAAACCGGACACTTGGTGCTATCCACTTTGCACACCAACAGCGC
ACCGGAAACTCTGACGCGCTTACTAAACATGGGCGTACCACCGTTTAACATTGCTTCGTCAGTACACTTGATTGTGGCGC
AACGCCTTGCACGGCGGTTGTGTGAAAAATGCAAACGCCCTGCTACCAATATTCCGCCCGAAGCGTTACTGGATCTGGGA
TTTAAAGCCGAAGAAATCCCCAGCCTCACGGTTTACGAACCCTGCGGCTGCGAGGAATGTTCCCACGGTTACAAAGGTCG
GGTCGGGCTTTACCAAGTCATGCCAATCTCGGCGGAAATGGGGCGCATGGTAATGAATGGCTGTAACTCAATGGAATTGG
CAGACCAATCCCTCAAAGAAGGCATTTTCGATTTACGTCAATCGGGTTTGGAAAAAGTACGCCTCGGCATTACCTCGTTA
GCGGAACTGTCGCGCGTCACCACCGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

49.565

100

0.501
  pilB Acinetobacter baylyi ADP1

50.536

98.418

0.497
  pilB Legionella pneumophila strain ERS1305867

48.522

100

0.49
  pilF Neisseria gonorrhoeae MS11

47.687

98.77

0.471
  pilB Vibrio parahaemolyticus RIMD 2210633

45.664

99.297

0.453
  pilB Vibrio campbellii strain DS40M4

45.75

97.188

0.445
  pilB Vibrio cholerae strain A1552

48.622

89.279

0.434
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40

97.54

0.39
  pilF Thermus thermophilus HB27

38.251

96.485

0.369