Detailed information    

insolico Bioinformatically predicted

Overview

Name   clpP   Type   Regulator
Locus tag   J2N67_RS28780 Genome accession   NZ_CP072691
Coordinates   5568453..5569034 (+) Length   193 a.a.
NCBI ID   WP_000991610.1    Uniprot ID   A0A9W5QF48
Organism   Bacillus thuringiensis strain IMBL-B9     
Function   degradation of ComK; degradation of DegU (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 5536888..5571315 5568453..5569034 within 0


Gene organization within MGE regions


Location: 5536888..5571315
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J2N67_RS28605 (J2N67_005728) - 5536888..5537622 (+) 735 WP_000135280.1 N-acetylmuramoyl-L-alanine amidase -
  J2N67_RS28610 (J2N67_005729) - 5538051..5538881 (+) 831 WP_000540614.1 GH25 family lysozyme -
  J2N67_RS28615 - 5539034..5539231 (+) 198 WP_001246231.1 hypothetical protein -
  J2N67_RS28620 (J2N67_005730) istB 5539378..5540130 (-) 753 WP_000798699.1 IS21-like element IS232 family helper ATPase IstB -
  J2N67_RS28625 (J2N67_005731) istA 5540120..5541415 (-) 1296 WP_000275580.1 IS21-like element IS232 family transposase -
  J2N67_RS28635 (J2N67_005734) - 5542752..5543204 (-) 453 WP_000877572.1 hypothetical protein -
  J2N67_RS28640 - 5543400..5544742 (+) 1343 WP_087970913.1 IS3 family transposase -
  J2N67_RS28645 (J2N67_005737) - 5544811..5545167 (-) 357 WP_000606231.1 hypothetical protein -
  J2N67_RS28650 (J2N67_005738) - 5545332..5545955 (-) 624 WP_000287726.1 hypothetical protein -
  J2N67_RS28655 (J2N67_005740) recQ 5547079..5549196 (+) 2118 WP_000494270.1 DNA helicase RecQ -
  J2N67_RS28660 (J2N67_005741) - 5549222..5549602 (+) 381 WP_000274916.1 gamma-glutamylcyclotransferase -
  J2N67_RS28665 - 5549657..5549971 (-) 315 Protein_5605 CPBP family intramembrane glutamate endopeptidase -
  J2N67_RS28670 (J2N67_005743) - 5550291..5552366 (+) 2076 WP_000065177.1 UvrD-helicase domain-containing protein -
  J2N67_RS28675 (J2N67_005744) - 5552504..5553064 (-) 561 WP_000337114.1 GNAT family protein -
  J2N67_RS28680 (J2N67_005745) - 5553286..5554014 (-) 729 WP_000360495.1 hypothetical protein -
  J2N67_RS28685 (J2N67_005747) - 5554901..5555248 (+) 348 WP_000369372.1 PadR family transcriptional regulator -
  J2N67_RS28690 (J2N67_005748) - 5555235..5556236 (+) 1002 WP_001048878.1 hypothetical protein -
  J2N67_RS28695 (J2N67_005749) - 5556317..5556829 (+) 513 WP_000617511.1 GNAT family N-acetyltransferase -
  J2N67_RS28700 (J2N67_005750) - 5556957..5557733 (+) 777 WP_000450752.1 serine hydrolase -
  J2N67_RS28705 (J2N67_005751) - 5557730..5558101 (+) 372 WP_000827980.1 hypothetical protein -
  J2N67_RS28710 (J2N67_005752) - 5558130..5558546 (+) 417 WP_000680418.1 GNAT family N-acetyltransferase -
  J2N67_RS28715 (J2N67_005753) - 5558582..5558986 (+) 405 WP_000288971.1 GNAT family N-acetyltransferase -
  J2N67_RS28720 (J2N67_005754) - 5559002..5559355 (+) 354 WP_000994623.1 MmcQ/YjbR family DNA-binding protein -
  J2N67_RS28725 (J2N67_005755) - 5559377..5560084 (+) 708 WP_000655256.1 class I SAM-dependent methyltransferase -
  J2N67_RS28730 (J2N67_005756) - 5560150..5560536 (+) 387 WP_001056436.1 DUF2809 domain-containing protein -
  J2N67_RS28735 (J2N67_005757) - 5560561..5561490 (+) 930 WP_000434097.1 phosphotransferase -
  J2N67_RS28740 (J2N67_005758) - 5561487..5561990 (+) 504 WP_000720552.1 DNA topology modulation protein -
  J2N67_RS28745 (J2N67_005759) - 5562152..5562832 (+) 681 WP_000180359.1 SUMF1/EgtB/PvdO family nonheme iron enzyme -
  J2N67_RS28750 (J2N67_005760) - 5562925..5564118 (+) 1194 WP_000124885.1 macrolide family glycosyltransferase -
  J2N67_RS28755 (J2N67_005761) - 5564572..5565939 (+) 1368 WP_001065165.1 lytic polysaccharide monooxygenase -
  J2N67_RS28760 (J2N67_005762) - 5566066..5566500 (-) 435 WP_001190864.1 GNAT family N-acetyltransferase -
  J2N67_RS28765 (J2N67_005763) rpiA 5566615..5567277 (-) 663 WP_001049986.1 ribose 5-phosphate isomerase A -
  J2N67_RS28770 (J2N67_005764) - 5567282..5567602 (-) 321 WP_001125388.1 2Fe-2S iron-sulfur cluster-binding protein -
  J2N67_RS28775 (J2N67_005765) - 5567745..5568431 (+) 687 WP_000337520.1 RNA polymerase subunit sigma-70 -
  J2N67_RS28780 (J2N67_005766) clpP 5568453..5569034 (+) 582 WP_000991610.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP Regulator
  J2N67_RS28785 (J2N67_005767) - 5569081..5569938 (-) 858 WP_001227621.1 glycine betaine ABC transporter substrate-binding protein -
  J2N67_RS28790 (J2N67_005768) - 5570110..5571315 (+) 1206 WP_000370606.1 glycine betaine/L-proline ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 193 a.a.        Molecular weight: 21192.45 Da        Isoelectric Point: 5.0776

>NTDB_id=555223 J2N67_RS28780 WP_000991610.1 5568453..5569034(+) (clpP) [Bacillus thuringiensis strain IMBL-B9]
MNAIPYVVEQTKLGERSYDIYSRLLKDRIIIIGSEINDQVASSVVAQLLFLEAEDAEKDIFLYINSPGGSTTAGFAILDT
MNLIKPDVQTLCMGFAASFGALLLLSGAKGKRFALPNSEIMIHQPLGGAQGQATEIEITAKRILKLKHDINKMIAEKTGQ
PIERVAHDTERDYFMTAEEAKAYGIVDDVVTKK

Nucleotide


Download         Length: 582 bp        

>NTDB_id=555223 J2N67_RS28780 WP_000991610.1 5568453..5569034(+) (clpP) [Bacillus thuringiensis strain IMBL-B9]
ATGAATGCAATTCCATATGTAGTAGAACAAACAAAATTAGGAGAACGTTCCTATGATATATATTCAAGGTTATTAAAAGA
CCGCATTATTATTATCGGCTCAGAAATTAATGATCAAGTAGCGAGTAGTGTCGTAGCGCAATTATTATTTTTAGAAGCAG
AAGATGCAGAAAAAGATATATTCTTATACATCAATAGCCCTGGCGGTTCAACGACAGCAGGTTTTGCAATATTAGATACG
ATGAATTTAATTAAACCAGATGTGCAAACGCTATGCATGGGCTTTGCGGCATCATTCGGTGCATTGTTATTATTATCAGG
AGCAAAAGGAAAACGGTTTGCTCTCCCTAACAGTGAAATTATGATTCATCAGCCGCTTGGTGGTGCACAAGGGCAAGCAA
CAGAAATTGAAATAACTGCGAAAAGAATATTAAAGTTAAAGCATGATATTAATAAAATGATTGCAGAAAAAACGGGGCAA
CCGATTGAAAGAGTAGCACATGATACAGAAAGAGATTATTTTATGACTGCAGAAGAAGCGAAGGCATATGGGATTGTAGA
TGACGTTGTTACGAAAAAATAA

Domains

Predicted by InterproScan.

Clp protease (CLP_protease)

(14-192)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  clpP Bacillus subtilis subsp. subtilis str. 168

68.586

98.964

0.679
  clpP Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

67.196

97.927

0.658
  clpP Streptococcus pyogenes JRS4

56.085

97.927

0.549
  clpP Streptococcus pyogenes MGAS315

56.085

97.927

0.549
  clpP Streptococcus pneumoniae R6

55.208

99.482

0.549
  clpP Streptococcus pneumoniae TIGR4

55.208

99.482

0.549
  clpP Streptococcus pneumoniae Rx1

55.208

99.482

0.549
  clpP Streptococcus thermophilus LMG 18311

55.208

99.482

0.549
  clpP Streptococcus thermophilus LMD-9

55.208

99.482

0.549
  clpP Streptococcus pneumoniae D39

55.208

99.482

0.549
  clpP Streptococcus mutans UA159

53.968

97.927

0.528
  clpP Lactococcus lactis subsp. cremoris KW2

52.381

97.927

0.513
  clpP Lactococcus lactis subsp. lactis strain DGCC12653

50.265

97.927

0.492