Detailed information NTG0554110

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: J7649_RS03075
• Genome accession: NZ_CP072549
• Coordinates: 640700..641896 (+)
• Length: 398 a.a.
• NCBI ID: WP_219309311.1
• Uniprot ID: -
• Organism: Acinetobacter lwoffii strain H7
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IScluster/Tn	635119..640134	640700..641896	flank	566

Gene organization within MGE regions

Location: 635119..641896

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J7649_RS03055 (J7649_03055)	-	635119..636453 (-)	1335	Protein_600	ISNCY-like element ISAba32 family transposase	-
J7649_RS03060 (J7649_03060)	-	636601..637926 (-)	1326	WP_081400761.1	ISNCY family transposase	-
J7649_RS03065 (J7649_03065)	-	638081..638638 (-)	558	WP_219309307.1	hypothetical protein	-
J7649_RS03070 (J7649_03070)	-	638797..640134 (+)	1338	WP_219309309.1	ISNCY family transposase	-
J7649_RS03075 (J7649_03075)	pilU	640700..641896 (+)	1197	WP_219309311.1	PilT/PilU family type 4a pilus ATPase	Machinery gene

Sequence

Protein

• Length: 398 a.a.
• Molecular weight: 45065.65 Da
• Isoelectric Point: 6.7418

>NTDB_id=554110 J7649_RS03075 WP_219309311.1 640700..641896(+) (pilU) [Acinetobacter lwoffii strain H7]
MFTAELLDEAKKFMHHMLTKVVEYGGSDLFITADFPPSIKHQGLMKPFGQQELTAEKTKLFAYSLMNDKQRKEFETELEC
NFAITVPGVSRFRVNVFQQQLNVGMVIRTITAEIPNFQKLKLPESLKHVIMEKRGLVLVVGGTGSGKSTSLAAMIDYRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
SNNANQTLDRIINFFPEERRNQLLMDLSSNMKAIISQRLIRTQDGKGRRAAIEIMLNTPLISDLILKGEFHELKAIMAKS
RELGMQTFDQALFDLYNQGAISYEEALRNADSTNELRLQIKLKSNRQDTTVATEATQFTMMQEPKAEEKQAENTSVSE

Nucleotide

• Length: 1197 bp

>NTDB_id=554110 J7649_RS03075 WP_219309311.1 640700..641896(+) (pilU) [Acinetobacter lwoffii strain H7]
ATGTTCACGGCAGAATTGTTGGACGAAGCAAAAAAATTCATGCATCACATGCTGACTAAGGTGGTGGAATATGGCGGTTC
AGATTTATTTATTACGGCTGATTTTCCACCTAGTATTAAACATCAGGGCTTAATGAAACCTTTTGGACAGCAGGAACTCA
CGGCTGAAAAAACCAAGCTGTTTGCCTACAGCTTGATGAATGATAAACAGCGTAAAGAATTTGAAACTGAACTCGAGTGC
AATTTTGCCATCACCGTGCCAGGTGTTTCCCGTTTCCGGGTCAATGTGTTCCAGCAACAACTCAATGTCGGCATGGTAAT
TCGTACCATTACTGCAGAAATTCCAAACTTCCAGAAACTGAAATTACCGGAATCGCTGAAACATGTGATTATGGAAAAAC
GCGGACTGGTGCTGGTGGTCGGTGGAACGGGTTCAGGTAAATCAACTTCACTCGCGGCGATGATTGACTACCGTAATGAA
AATTCGGCCGGGCATATCATTACCGTAGAAGACCCGGTTGAATATGTGCACAAGCATAAAAAATCCATGATTACTCATCG
TGAAGTCGGAGTGGACTGCCATTCCTGGCACAACGCGCTGAAAAATACCTTGCGTCAGGCACCAGATGTGATTCTGATCG
GGGAAATTCGCGATACTGAAACTATGGAACATGCGATTGCCTTTGCGGAAACTGGTCATTTGTGTTTGGGAACACTGCAC
TCCAACAATGCCAATCAGACTTTGGACCGAATTATTAACTTCTTTCCGGAAGAACGCCGGAATCAGTTATTAATGGATTT
GTCATCCAATATGAAGGCAATTATTTCACAGCGTCTGATTCGTACTCAAGATGGTAAAGGCCGTCGCGCAGCGATTGAAA
TCATGTTGAATACACCACTTATTTCAGACTTGATTCTGAAAGGTGAATTCCATGAGCTGAAAGCAATTATGGCGAAATCG
CGTGAACTGGGCATGCAGACTTTTGACCAGGCCTTATTTGACCTGTATAACCAAGGCGCCATTTCCTATGAAGAAGCTTT
GCGTAATGCTGATTCTACCAATGAATTACGTTTGCAGATCAAGCTGAAAAGTAACCGACAGGACACTACCGTAGCCACAG
AGGCAACCCAATTCACCATGATGCAAGAGCCAAAAGCGGAAGAGAAACAAGCAGAGAATACTTCGGTGTCAGAATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (118-282)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.207	87.437	0.535
pilU	Acinetobacter baylyi ADP1	56.196	87.186	0.49
pilU	Vibrio cholerae strain A1552	52.676	89.196	0.47
pilT	Pseudomonas aeruginosa PAK	44.479	81.91	0.364
pilT	Acinetobacter nosocomialis M2	43.636	82.915	0.362
pilT	Acinetobacter baumannii D1279779	43.636	82.915	0.362
pilT	Acinetobacter baumannii strain A118	43.636	82.915	0.362