Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   J5N61_RS01950 Genome accession   NZ_CP071973
Coordinates   419148..420875 (-) Length   575 a.a.
NCBI ID   WP_212387962.1    Uniprot ID   -
Organism   Acinetobacter junii strain INC8271     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 414148..425875
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5N61_RS01920 (J5N61_01920) - 414232..414558 (+) 327 WP_004958605.1 pyrimidine/purine nucleoside phosphorylase -
  J5N61_RS01925 (J5N61_01925) rlmB 414748..415497 (+) 750 WP_004916859.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  J5N61_RS01930 (J5N61_01930) - 415528..416439 (+) 912 WP_004958607.1 DMT family transporter -
  J5N61_RS01935 (J5N61_01935) coaE 416436..417035 (-) 600 WP_004958609.1 dephospho-CoA kinase -
  J5N61_RS01940 (J5N61_01940) pilD 417037..417897 (-) 861 WP_204379161.1 prepilin peptidase Machinery gene
  J5N61_RS01945 (J5N61_01945) pilC 417897..419123 (-) 1227 WP_212387959.1 type II secretion system F family protein Machinery gene
  J5N61_RS01950 (J5N61_01950) pilB 419148..420875 (-) 1728 WP_212387962.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J5N61_RS01955 (J5N61_01955) tpiA 421221..422012 (+) 792 WP_004958617.1 triose-phosphate isomerase -
  J5N61_RS01960 (J5N61_01960) secG 422025..422354 (+) 330 WP_004916847.1 preprotein translocase subunit SecG -
  J5N61_RS01980 (J5N61_01980) rimP 422977..423501 (+) 525 WP_004916844.1 ribosome maturation factor RimP -
  J5N61_RS01985 (J5N61_01985) nusA 423539..425023 (+) 1485 WP_004916840.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63694.84 Da        Isoelectric Point: 5.2088

>NTDB_id=549542 J5N61_RS01950 WP_212387962.1 419148..420875(-) (pilB) [Acinetobacter junii strain INC8271]
MSGIHTSPKFSGFIRRIVDDGHIAASNMQSAIDAAKKAQQDIVAHLIEHHRLSPLTIAESISLEFGEPLFDLAVFDTALL
PREITDSKLIQKHRVVPLIQRGQILYVATSNPTNIDALDAIRFNTKLNIEAVIVEHNKLEKLIDQQFGDSGSFDFGDSED
FDLDNVDVASTDTNNLTEDNDDTNHQDESPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIANPPLQLA
TRLASRLKVMSQMDITEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDEQKELFMS
ALDKPQGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNPKTGLTFSAALKAFLRQDPDVIM
VGEIRDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVQSFNIATSVNLVIAQRLARRLCSSCKQPIDIPKQSL
LEMGFTEQDLTTPGFQIFQPVGCDACREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKAGFDNLRRSGLKKVM
QGITSLQEVNRVTNE

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=549542 J5N61_RS01950 WP_212387962.1 419148..420875(-) (pilB) [Acinetobacter junii strain INC8271]
ATGTCAGGAATACATACGTCTCCAAAATTTTCGGGGTTTATTCGACGCATAGTCGATGATGGTCACATTGCTGCATCAAA
TATGCAGAGTGCGATTGATGCTGCAAAAAAAGCACAACAAGATATTGTTGCTCACTTAATTGAACATCATCGTCTATCAC
CTCTGACAATTGCGGAAAGCATATCTTTAGAGTTTGGTGAGCCACTCTTTGATCTTGCTGTATTTGATACAGCTCTATTA
CCTCGTGAAATAACTGACAGCAAACTGATTCAAAAACATCGTGTTGTTCCACTCATTCAACGTGGTCAGATTTTGTATGT
TGCGACCAGCAACCCGACGAATATTGATGCATTAGACGCAATCAGGTTCAATACCAAGTTGAATATCGAAGCTGTGATTG
TTGAGCACAATAAACTCGAAAAACTGATTGATCAGCAATTTGGAGATAGCGGCAGTTTCGATTTTGGAGATAGTGAAGAC
TTTGATTTAGATAATGTAGATGTTGCATCTACTGATACAAATAACTTGACTGAAGATAACGATGATACAAACCACCAAGA
CGAATCACCTATCGTTAAATATATCAATAAACTGTTGATTGATGCGATTCGAATGGGAGCATCTGACTTACATTTTGAAC
CTTATGAAAAAATGTATAGAGTGCGTTATCGTGTGGATGGTGTACTCAGACAAATTGCTAACCCACCATTACAGCTCGCA
ACTCGTTTAGCCTCGCGCTTAAAAGTCATGTCTCAGATGGACATTACTGAAAAACGTATGCCCCAAGATGGTCGTATCAA
ACTTAAACTCTCTAAAACTAAAGCAATTGATTTTCGTGTTAACTCACTTCCAACTTTATTTGGAGAAAAACTGGTTCTTC
GTATCTTAGATCCTTCAAGCGCAATGTTAGGTATTGAAGCATTAGGCTATGAGGATGAACAAAAAGAACTCTTTATGAGT
GCATTAGACAAGCCTCAGGGTATGCTTCTAATTACAGGTCCAACAGGCTCAGGTAAAACTGTATCGTTGTATACGGGTTT
AAATATCTTAAATACAGAAGATACCAATATTTCGACCGCAGAAGATCCCGTCGAAATTAACTTAGAAGGTATTAACCAAG
TTAACGTCAACCCTAAAACAGGTTTAACTTTCTCAGCAGCATTAAAAGCTTTTTTACGTCAAGATCCTGATGTCATCATG
GTCGGTGAGATTCGTGATTTAGAAACAGCTGAAATTGCTATCAAAGCAGCCCAAACAGGTCACATGGTGATGTCGACTTT
GCACACCAATAGCGCTCCAGAAACACTCACACGTTTACGTAATATGGGAGTTCAGTCTTTCAATATTGCGACTTCGGTTA
ATCTAGTAATTGCTCAACGTTTAGCTCGCCGCCTATGTTCTTCATGCAAACAACCTATCGATATTCCTAAACAAAGCCTT
CTAGAAATGGGTTTCACCGAACAAGACTTAACCACCCCAGGTTTCCAAATATTTCAACCTGTAGGCTGTGATGCTTGTCG
TGAAGGATATAAAGGACGTGTCGGTATTTATGAAGTGATGAAAGTGACACCAGAGATTTCCAAGATTATTATGGAAGATG
GGAATGCACTACAAATTGCAGCAGCATCTGAAAAGGCTGGTTTCGATAATTTACGTCGTTCTGGTTTAAAAAAGGTCATG
CAAGGGATCACCTCTTTGCAAGAAGTTAACCGAGTAACAAATGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(197-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.522

100

0.805
  pilB Acinetobacter baylyi ADP1

76.174

100

0.762
  pilB Legionella pneumophila strain ERS1305867

52.602

93.565

0.492
  pilB Vibrio cholerae strain A1552

49.281

96.696

0.477
  pilB Vibrio parahaemolyticus RIMD 2210633

49.104

97.043

0.477
  pilF Neisseria gonorrhoeae MS11

50

95.304

0.477
  pilB Vibrio campbellii strain DS40M4

50.958

90.783

0.463
  pilB/pilB1 Synechocystis sp. PCC 6803

34.416

100

0.369