Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   J5I97_RS04815 Genome accession   NZ_CP071955
Coordinates   1035106..1036842 (+) Length   578 a.a.
NCBI ID   WP_208589545.1    Uniprot ID   -
Organism   Xanthomonas fragariae strain YL19     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1030106..1041842
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5I97_RS04790 coaE 1030956..1031588 (-) 633 WP_208589532.1 dephospho-CoA kinase -
  J5I97_RS04795 - 1031602..1032465 (-) 864 WP_208589534.1 A24 family peptidase -
  J5I97_RS04800 pilC 1032473..1033729 (-) 1257 WP_208591561.1 type II secretion system F family protein Machinery gene
  J5I97_RS04805 pilA 1034097..1034507 (+) 411 WP_208589542.1 pilin Machinery gene
  J5I97_RS04810 pilA/pilAI 1034582..1034989 (+) 408 WP_208589544.1 pilin Machinery gene
  J5I97_RS04815 pilB 1035106..1036842 (+) 1737 WP_208589545.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J5I97_RS04820 - 1036889..1037485 (-) 597 WP_208589553.1 Fic family protein -
  J5I97_RS04825 - 1037482..1037664 (-) 183 WP_208589555.1 YhfG family protein -
  J5I97_RS04830 pilR 1037739..1039187 (-) 1449 WP_208591562.1 sigma-54 dependent transcriptional regulator Regulator
  J5I97_RS04835 - 1039416..1041029 (-) 1614 WP_208589557.1 HAMP domain-containing sensor histidine kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62574.82 Da        Isoelectric Point: 5.6024

>NTDB_id=549396 J5I97_RS04815 WP_208589545.1 1035106..1036842(+) (pilB) [Xanthomonas fragariae strain YL19]
MSAVTSANLVGITGIARRLVQDGALEETVARVAMDQAAAAKIPLPQWFSEKKLVSASQLAAANAVEFGMPLMDVSVFDAS
QNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSLG
DDDEGMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKMLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTAEQVAAGIELYEAVGCDECTAGYKGRTGIYQVMPMTEEIGAIVLEGGNAMQIAEAAQSIGIKDLRQSALI
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=549396 J5I97_RS04815 WP_208589545.1 1035106..1036842(+) (pilB) [Xanthomonas fragariae strain YL19]
ATGAGCGCGGTCACTTCTGCTAATTTGGTCGGCATTACCGGCATAGCCAGACGCCTAGTTCAGGACGGCGCCTTGGAAGA
AACGGTGGCGCGGGTTGCAATGGACCAGGCTGCGGCAGCTAAGATTCCTCTGCCACAATGGTTTTCAGAGAAAAAGTTGG
TTTCGGCTTCACAACTGGCTGCTGCCAACGCAGTCGAGTTCGGCATGCCGCTGATGGACGTGTCGGTGTTCGACGCTAGC
CAGAATGCGGTCAAGCTGGTCAGTGAGGAGTTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAATCGGTT
ATTCGTGGGGGTGAGCAACCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCACACCAATCTGGTGGTGGAGCCGA
TCTTGGTCGACGAGGACCAGATCCGTCGCACCCTGGAGCAGTGGCAGGCCAGCAATGCGGCGCTTGGCTCGTCGCTCGGT
GACGACGATGAGGGGATGGGGGACCTGGACGTGTCGGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGATGCTGGTGGATGCGATCCGGCGGGGGGCCTCCGACA
TCCATTTCGAGCCGTATGAAGACGATTACCGGGTGCGGTTGCGGATCGATGGGCTGCTGAAGAACGTGGCCAAGGCGCCG
GTGAAACTGAGCCAGCGCATCGCGGCGCGCTTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAACGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAATCTGTCCAAGACCAAGCAGATCGACTTCCGTGTCAGCACCTTGCCGACCTTGTTCGGCGAGA
AGGTGGTGCTGCGTATTTTGGACGCTAGCGCGGCCAAGCTGGGCATCGAGAAGTTGGGCTATGAGGCGGATCAGCAGAAG
CTGTTCCTGGATGCGATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGTT
GTATACGGCGCTGGGCATCCTCAACGATGAGACGCGCAATATCTCCACGGCCGAGGATCCGGTGGAAATCCGCTTGCCGG
GCGTGAACCAGGTGCAGCAGAACGTCAAGCGGGGCATGACGTTTGCGGCGGCGTTGCGGTCGTTTCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAAACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGCCACATGGT
GTTGTCGACGTTGCACACCAACGATGCGCCGCAGACCATTGCGCGTCTGATGAACATGGGCATTGCGCCGTACAACATTA
CCTCATCGGTGACGTTGGTCATTGCACAGCGTCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGTCGACGTTGCCC
GAGCACGCATTGCTTGCGGAAGGATTCACCGCAGAGCAGGTGGCTGCAGGTATCGAACTATATGAGGCGGTGGGCTGCGA
CGAGTGCACCGCAGGCTATAAGGGCCGTACCGGTATTTACCAGGTGATGCCGATGACCGAAGAGATCGGCGCGATCGTGC
TGGAAGGCGGCAATGCAATGCAGATCGCCGAGGCGGCGCAGAGTATCGGCATCAAAGATCTGCGGCAATCGGCGCTGATC
AAGGCGGCGCATGGGGTGACCAGCCTGGCCGAGATCAATCGGGTGACGAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.268

100

0.554
  pilB Acinetobacter baylyi ADP1

54.75

100

0.548
  pilB Legionella pneumophila strain ERS1305867

52.9

98.443

0.521
  pilB Vibrio cholerae strain A1552

48.7

99.827

0.486
  pilF Neisseria gonorrhoeae MS11

48.768

98.27

0.479
  pilB Vibrio campbellii strain DS40M4

46.221

98.443

0.455
  pilB Vibrio parahaemolyticus RIMD 2210633

46.786

96.886

0.453