Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   J4H94_RS02815 Genome accession   NZ_CP071899
Coordinates   620606..622708 (+) Length   700 a.a.
NCBI ID   WP_208217411.1    Uniprot ID   -
Organism   Stutzerimonas frequens strain TPA3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 615606..627708
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4H94_RS02795 (J4H94_02795) comM 617772..618836 (+) 1065 WP_208217409.1 pilus assembly protein PilM Machinery gene
  J4H94_RS02800 (J4H94_02800) - 618836..619408 (+) 573 WP_063539613.1 PilN domain-containing protein -
  J4H94_RS02805 (J4H94_02805) pilO 619405..620028 (+) 624 WP_208217410.1 type 4a pilus biogenesis protein PilO -
  J4H94_RS02810 (J4H94_02810) pilP 620025..620552 (+) 528 WP_102839586.1 type 4a pilus biogenesis lipoprotein PilP -
  J4H94_RS02815 (J4H94_02815) pilQ 620606..622708 (+) 2103 WP_208217411.1 type IV pilus secretin PilQ Machinery gene
  J4H94_RS02820 (J4H94_02820) aroK 622714..623259 (+) 546 WP_208217412.1 shikimate kinase AroK -
  J4H94_RS02825 (J4H94_02825) aroB 623282..624385 (+) 1104 WP_208217413.1 3-dehydroquinate synthase -
  J4H94_RS02830 (J4H94_02830) - 624396..625979 (+) 1584 WP_208217414.1 AAA family ATPase -

Sequence

Protein


Download         Length: 700 a.a.        Molecular weight: 75179.12 Da        Isoelectric Point: 4.7672

>NTDB_id=548882 J4H94_RS02815 WP_208217411.1 620606..622708(+) (pilQ) [Stutzerimonas frequens strain TPA3]
MNSTLSRLGISVLVAFVSPAVLAANLNALDVAALPGDRVELKLAFDEPVAPPRGYTLEQPARIALDLPGVSNNLGAKNRE
LGVGNARSVTVVEAQGRTRLIVNLTSLVPYSTRVDGKNLFVVLGENAGVSGPAAVSAQPSAVAQPSVTAAPAKAVAVGKA
IDNIDFRRGDDGAGNVIITLSDPSISSTIEEQGGKIRVNFAKAQLPESLRVRLDVQDFATPVRFVDSASQAGGASIAIEP
TGRYDYLAYQTDNKLTISVKPLTEDEAEKRKADRFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVSGNITLRL
QNVPWDQALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELESQRQIAELAPLRREVVQVNYAKAADIARLFQSVTDN
GGMAEERGSIAVDDRTNNIIAYQTQERLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGNLGAGDKWSAWG
KNGNLDVEDAEGGGQRITWPMNTPFVDMGAVGSTSGIGLGFVTDNAILDLQLSAMEKTGNGEVVSQPKVVTADKETAKIL
KGSEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDEPDFSQAATTGVPAIRKNEVNAKVLVTDGETI
VIGGVFSNTQSKSVDKVPFLGDLPFLGRLFRRDLVQDRKSELLVFITPRIMNNQAIQASR

Nucleotide


Download         Length: 2103 bp        

>NTDB_id=548882 J4H94_RS02815 WP_208217411.1 620606..622708(+) (pilQ) [Stutzerimonas frequens strain TPA3]
ATGAACAGTACTCTCTCGCGCCTCGGCATTTCAGTGCTTGTGGCGTTCGTCTCGCCGGCTGTACTGGCAGCCAATCTCAA
TGCCTTGGATGTGGCCGCTCTGCCGGGCGATCGAGTCGAGCTCAAGCTGGCTTTCGACGAGCCCGTTGCGCCGCCGCGTG
GTTACACCCTTGAACAGCCTGCTCGCATTGCCCTGGATCTGCCGGGTGTATCGAACAATCTGGGAGCGAAGAATCGTGAG
TTGGGGGTTGGTAATGCGCGTAGCGTTACGGTCGTTGAGGCGCAAGGACGTACTCGGTTGATTGTAAACCTCACTTCGCT
GGTGCCCTATTCCACTCGAGTGGATGGCAAGAATCTGTTCGTCGTGCTGGGCGAAAATGCCGGCGTGAGTGGCCCGGCGG
CGGTCTCAGCGCAGCCGAGCGCAGTGGCGCAGCCATCTGTTACGGCGGCTCCCGCGAAGGCGGTGGCTGTTGGTAAGGCC
ATTGACAACATTGACTTTCGGCGTGGCGATGATGGAGCCGGTAATGTGATTATTACGCTATCCGATCCATCAATCAGCTC
GACCATTGAGGAGCAGGGCGGAAAAATTCGCGTAAATTTCGCCAAGGCTCAGTTGCCGGAATCGCTGCGTGTGCGCCTGG
ATGTCCAAGACTTCGCTACGCCTGTCAGGTTCGTTGATTCCGCTTCTCAGGCAGGTGGTGCGAGCATCGCAATCGAGCCG
ACTGGTCGATATGACTATTTGGCCTATCAGACCGACAACAAGCTGACTATCAGCGTCAAACCTCTTACGGAAGACGAAGC
GGAAAAGCGTAAGGCTGATCGATTTGCTTATTCTGGCGAGAAGCTGTCGCTTAATTTCCAAGACATCGACGTTCGCTCGG
TATTGCAGCTAATCGCTGATTTCACGGATCTAAATCTGGTCGCAAGCGATACCGTTAGCGGCAATATCACGCTGCGTCTG
CAGAACGTGCCGTGGGATCAGGCTCTGGACTTGGTGCTTAAGACCAAGGGGTTGGACAAGCGCCAGGTCGGTAACGTGCT
GCTGGTGGCTCCTGCCGACGAAATTGCCGCACGTGAACGTCAGGAGCTTGAGTCTCAGCGGCAGATTGCCGAACTGGCTC
CGCTTCGCCGTGAGGTGGTGCAGGTTAACTATGCCAAGGCCGCTGACATCGCGAGGCTATTTCAGTCGGTAACGGATAAT
GGCGGGATGGCGGAAGAGCGGGGGTCTATTGCGGTTGATGATCGCACCAACAATATCATTGCCTACCAGACTCAGGAGCG
TCTCGATGAGTTGCGTCGTATCGTGGCTCAACTGGATATTCCGGTTCGGCAGGTAATGATTGAGGCGCGCATAGTTGAGG
CAAACGTCGACTACGATAAGAGCCTTGGTGTTCGTTGGGGCGGCAATCTCGGCGCTGGTGATAAGTGGAGTGCCTGGGGC
AAGAACGGCAATCTTGATGTTGAAGATGCTGAAGGGGGCGGTCAGCGAATCACCTGGCCAATGAATACGCCATTTGTCGA
CATGGGCGCGGTAGGCTCAACTTCGGGCATTGGGCTTGGGTTCGTGACCGATAACGCGATCTTGGATCTACAGCTATCCG
CAATGGAAAAAACCGGCAACGGCGAGGTTGTCTCGCAGCCTAAGGTAGTCACCGCAGACAAAGAAACTGCAAAGATCCTC
AAGGGCTCCGAAGTTCCTTATCAGGAGGCGAGCTCCAGTGGTGCAACGAGCACGTCGTTCAAGGAGGCAGCTCTCTCGCT
GGAAGTGACTCCGCAGATCACACCGGATAATCGCATCATCATGGAGGTCAAGGTCACTAAGGATGAGCCAGACTTCTCTC
AGGCAGCAACTACCGGAGTGCCGGCCATTCGCAAGAATGAGGTTAACGCAAAGGTTCTGGTAACTGATGGTGAGACGATC
GTAATCGGTGGCGTTTTCTCCAATACGCAGAGCAAGTCAGTGGACAAGGTGCCGTTCCTAGGGGATCTCCCGTTCTTGGG
TCGCCTGTTCCGTCGTGATCTGGTGCAGGATCGAAAGTCTGAACTGCTTGTGTTCATCACGCCGAGGATCATGAATAATC
AGGCGATTCAAGCCAGTCGCTGA

Domains

Predicted by InterProScan.

AMIN domain (AMIN)

(38-123)

Bacterial type II/III secretion system short domain (Secretin_N)

(378-444)

Secretin and TonB N terminus short domain (STN)

(305-351)

Bacterial type II and III secretion system protein (Secretin)

(534-691)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.853

100

0.759
  pilQ Acinetobacter baumannii strain A118

39.054

100

0.413
  pilQ Acinetobacter baumannii D1279779

39.054

100

0.413
  comQ Acinetobacter baylyi ADP1

41.168

100

0.413
  pilQ Legionella pneumophila strain ERS1305867

37.997

97

0.369
  pilQ Legionella pneumophila strain Lp02

37.703

97

0.366