Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   LSP17_RS29245 Genome accession   NZ_CP089062
Coordinates   6340397..6340936 (-) Length   179 a.a.
NCBI ID   WP_003116248.1    Uniprot ID   Q59651
Organism   Pseudomonas aeruginosa strain UNC_PaerCF38     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6335397..6345936
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSP17_RS29225 (LSP17_29230) - 6336081..6338336 (-) 2256 WP_019726810.1 DUF1631 domain-containing protein -
  LSP17_RS29230 (LSP17_29235) nadC 6338622..6339470 (+) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LSP17_RS29240 (LSP17_29245) - 6339635..6340333 (-) 699 WP_023121720.1 hypothetical protein -
  LSP17_RS29245 (LSP17_29250) pilE 6340397..6340936 (-) 540 WP_003116248.1 pilin Machinery gene
  LSP17_RS29250 (LSP17_29255) pilB 6341165..6342868 (+) 1704 WP_003116249.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LSP17_RS29255 (LSP17_29260) pilC 6342872..6344089 (+) 1218 WP_003116250.1 type II secretion system F family protein Machinery gene
  LSP17_RS29260 (LSP17_29265) pilD 6344092..6344964 (+) 873 WP_003116251.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  LSP17_RS29265 (LSP17_29270) coaE 6344961..6345572 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  LSP17_RS29270 (LSP17_29275) yacG 6345569..6345769 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 18003.27 Da        Isoelectric Point: 4.6894

>NTDB_id=548295 LSP17_RS29245 WP_003116248.1 6340397..6340936(-) (pilE) [Pseudomonas aeruginosa strain UNC_PaerCF38]
MTAQKGFTLIELMIVVAIIGILAAVALPAYQDYTIRARVTEGVGLAASAKTLIGDSSATAGELAASARVWNAQAGNAGAT
SKYVTSVQIAEATGEITVTFNAANVGNIPANSTLVFTPYVQNAAGAPTQLGASYASGVTGSIDWGCASDSNAVSSGTDRN
MPALTAGTLPARFAPSECR

Nucleotide


Download         Length: 540 bp        

>NTDB_id=548295 LSP17_RS29245 WP_003116248.1 6340397..6340936(-) (pilE) [Pseudomonas aeruginosa strain UNC_PaerCF38]
ATGACAGCTCAGAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATTCTTGCTGCCGTCGCTTT
GCCGGCATATCAGGATTACACCATTCGTGCTCGCGTGACAGAGGGGGTTGGCCTGGCTGCCAGCGCCAAGACGCTTATTG
GCGATAGCTCTGCCACTGCCGGTGAGCTAGCCGCTTCGGCAAGGGTCTGGAATGCTCAAGCCGGTAACGCCGGTGCTACC
AGTAAGTATGTGACCTCTGTACAAATTGCAGAGGCGACTGGTGAAATCACTGTTACTTTCAATGCCGCAAACGTGGGTAA
TATTCCGGCTAACTCTACCCTGGTATTTACTCCCTATGTGCAGAATGCTGCCGGTGCCCCGACTCAATTGGGTGCCAGTT
ATGCTTCCGGTGTGACTGGCTCTATTGACTGGGGTTGTGCATCGGACTCCAACGCGGTTTCCAGTGGTACCGACCGCAAT
ATGCCTGCCCTGACTGCAGGTACCCTGCCGGCTCGTTTCGCTCCTAGCGAATGCCGCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(36-179)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q59651

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

41.451

100

0.447
  pilA Ralstonia pseudosolanacearum GMI1000

44.444

100

0.447
  pilA2 Legionella pneumophila str. Paris

42.373

98.883

0.419
  pilA2 Legionella pneumophila strain ERS1305867

41.808

98.883

0.413
  comP Acinetobacter baylyi ADP1

38.889

100

0.391