Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   J4N43_RS02895 Genome accession   NZ_CP071842
Coordinates   594538..594966 (-) Length   142 a.a.
NCBI ID   WP_050912911.1    Uniprot ID   -
Organism   Vibrio sp. SCSIO 43009     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 589538..599966
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4N43_RS02875 (J4N43_02870) coaE 590057..590671 (-) 615 WP_158159769.1 dephospho-CoA kinase -
  J4N43_RS02880 (J4N43_02875) pilD 590672..591541 (-) 870 WP_158159768.1 A24 family peptidase Machinery gene
  J4N43_RS02885 (J4N43_02880) pilC 591606..592829 (-) 1224 WP_017635918.1 type II secretion system F family protein Machinery gene
  J4N43_RS02890 (J4N43_02885) pilB 592853..594538 (-) 1686 WP_158159767.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J4N43_RS02895 (J4N43_02890) pilA 594538..594966 (-) 429 WP_050912911.1 pilin Machinery gene
  J4N43_RS02900 (J4N43_02895) nadC 595232..596119 (-) 888 WP_158159766.1 carboxylating nicotinate-nucleotide diphosphorylase -
  J4N43_RS02905 (J4N43_02900) ampD 596212..596763 (+) 552 WP_042521337.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  J4N43_RS02910 (J4N43_02905) pdhR 597169..597936 (+) 768 WP_005379989.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14510.54 Da        Isoelectric Point: 5.7554

>NTDB_id=548203 J4N43_RS02895 WP_050912911.1 594538..594966(-) (pilA) [Vibrio sp. SCSIO 43009]
MKTNKQKKQQGFTLIELMIVVAVIGVLAAIAIPQYQNYVKKSEAAAGLATLRSLTTNIDTFIADTGTFPADGDAPTLGAA
VDMNKLGTISFADSGASGATATFTFDGTASALASTDTVVLAKDATTGLWTCSHSTGVTLKGC

Nucleotide


Download         Length: 429 bp        

>NTDB_id=548203 J4N43_RS02895 WP_050912911.1 594538..594966(-) (pilA) [Vibrio sp. SCSIO 43009]
ATGAAAACGAATAAACAGAAGAAGCAGCAAGGTTTTACCCTAATTGAATTGATGATCGTGGTAGCGGTTATTGGTGTTTT
AGCAGCGATTGCTATTCCACAGTATCAGAATTATGTAAAAAAATCAGAGGCTGCAGCTGGGCTTGCAACTCTTCGTTCAC
TAACTACAAATATTGATACTTTCATTGCTGATACTGGCACCTTTCCTGCTGATGGCGACGCACCCACGTTAGGTGCTGCT
GTTGATATGAACAAACTTGGAACAATTTCTTTTGCAGATAGTGGTGCATCGGGAGCTACAGCAACGTTTACTTTTGATGG
TACAGCTAGCGCACTAGCTTCTACTGATACCGTTGTCCTTGCGAAGGATGCAACTACCGGACTTTGGACCTGCTCTCATA
GTACTGGCGTAACTCTAAAAGGCTGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(41-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

51.299

100

0.556
  pilA Vibrio cholerae strain A1552

51.299

100

0.556
  pilA Vibrio cholerae C6706

51.299

100

0.556
  pilA Vibrio parahaemolyticus RIMD 2210633

48.252

100

0.486
  pilA Pseudomonas aeruginosa PAK

45.27

100

0.472
  pilA Acinetobacter baumannii strain A118

35.948

100

0.387