Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JZM58_RS25505 Genome accession   NZ_CP071797
Coordinates   5737685..5739385 (+) Length   566 a.a.
NCBI ID   WP_207983961.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain YK-310     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5732685..5744385
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JZM58_RS25485 (JZM58_25485) - 5732961..5735912 (+) 2952 WP_207983960.1 DUF748 domain-containing protein -
  JZM58_RS25490 (JZM58_25490) - 5735912..5736208 (+) 297 WP_007959599.1 DUF2845 domain-containing protein -
  JZM58_RS25495 (JZM58_25495) - 5736375..5736731 (-) 357 WP_085608680.1 BON domain-containing protein -
  JZM58_RS25500 (JZM58_25500) - 5737042..5737458 (-) 417 WP_011335978.1 pilin -
  JZM58_RS25505 (JZM58_25505) pilB 5737685..5739385 (+) 1701 WP_207983961.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JZM58_RS25510 (JZM58_25510) pilC 5739388..5740605 (+) 1218 WP_207983962.1 type II secretion system F family protein Machinery gene
  JZM58_RS25515 (JZM58_25515) pilD 5740607..5741479 (+) 873 WP_207983963.1 A24 family peptidase Machinery gene
  JZM58_RS25520 (JZM58_25520) coaE 5741476..5742099 (+) 624 WP_207983964.1 dephospho-CoA kinase -
  JZM58_RS25525 (JZM58_25525) yacG 5742096..5742296 (+) 201 WP_011335983.1 DNA gyrase inhibitor YacG -
  JZM58_RS25530 (JZM58_25530) - 5742303..5742518 (-) 216 WP_207983965.1 hypothetical protein -
  JZM58_RS25535 (JZM58_25535) - 5742584..5743273 (-) 690 WP_115079246.1 energy-coupling factor ABC transporter permease -
  JZM58_RS25540 (JZM58_25540) - 5743407..5744033 (+) 627 WP_007959580.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62818.91 Da        Isoelectric Point: 5.9468

>NTDB_id=547837 JZM58_RS25505 WP_207983961.1 5737685..5739385(+) (pilB) [Pseudomonas fluorescens strain YK-310]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRNRLSLVSYLVQNKLVKSWQVAEIASEHFGMAFLDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTDAIEKFFDTHATGLEEMADVDLDG
LDIESADDGKQDTLGGIDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVMRILDPSSAQIGIDALGYEPEQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGMDFAQALRSFLRQDPDVIMVGEIRDI
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKPIEIPRETLIKEGFPE
ERIGHFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDIQMRRDGFNDLRTSGLIKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=547837 JZM58_RS25505 WP_207983961.1 5737685..5739385(+) (pilB) [Pseudomonas fluorescens strain YK-310]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAACAACTGGTCCAGGCCGAATTGCTCACGGAAAAGAGTGCCCAGCAAGC
CTGGCAGCAGGCGCAGCGCAATCGCCTGTCGCTGGTCAGTTACCTGGTGCAGAACAAACTGGTGAAGAGCTGGCAGGTCG
CCGAAATAGCCTCGGAGCATTTCGGCATGGCATTTCTCGACCTCAACTGTCTCGACAAGGAAACCCAGCCCAAGGGCCTG
GTCAGTGAAAAACTGGTGCGCCAGCACCACGCCCTGCCCCTCTGGCGGCGCGGCAACAAACTGTTCGTGGGAATTTCCGA
CCCGAGCAATCATCAGGCGATCAATGACATTCAGTTCAGCACCGGGCTGAGCACCGAAGCCATTCTGGTCGAGGACGACA
AACTCACCGACGCCATCGAAAAGTTCTTCGACACCCACGCCACCGGCCTGGAGGAGATGGCCGATGTCGACCTCGACGGT
CTGGACATCGAGTCGGCCGACGACGGCAAGCAAGACACGCTCGGCGGAATCGACGCCGACGATGCCCCGGTGGTGCGCTT
CGTGCACAAGATGCTGCTGGACGCGATCAAGAGCGGCTCATCCGACCTGCATTTCGAGCCTTACGAAAAGAACTACCGGG
TGCGGGTACGTACCGACGGCATTCTGCGGGAGGTGGCCAAGCCGCCGATTCAGCTCGCCGGGCGCATTGCCGCACGCCTG
AAGGTCATGGCCAGCCTCGACATTTCAGAACGGCGCAAACCCCAGGACGGGCGGATCAAGATGCGTCTGTCGAAGAGCAA
ATCGATCGATTTCCGGGTCAACACCCTGCCCACGCTCTGGGGCGAGAAAGTGGTAATGCGGATCCTCGACCCGTCCAGTG
CCCAGATCGGCATCGATGCCCTCGGTTACGAGCCGGAGCAGAAAGACCTGTACATGGCCGCCCTCAAACAGCCGCAAGGC
ATGATTCTGGTAACGGGCCCCACCGGCTCGGGCAAAACCGTGTCGCTGTACACCGGGCTGAACATCCTCAACACCGTCGA
TATCAACATCTCCACCGCCGAAGATCCGGTAGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCACGTCAGG
GGATGGACTTTGCCCAGGCCCTGCGCTCGTTCCTGCGTCAGGATCCGGACGTGATCATGGTCGGCGAGATCCGCGACATC
GAAACCGCCGAAATCGCGATCAAGGCCGCCCAGACCGGGCACCTCGTGCTGTCCACCCTGCATACCAACAGCGCAGCGGA
AACCCTCACCCGTTTGCACAACATGGGCATTCCCGGCTTCAACATCGCGACCTCGGTGAGCCTGATCATTGCCCAGCGTC
TGGCGCGAAAACTCTGCAGCCACTGCAAGAAACCCATCGAGATCCCCCGCGAAACCCTGATCAAGGAAGGTTTCCCCGAG
GAACGCATCGGCCATTTCACGATCTATGAGCCCGTTGGTTGCGATCACTGCAACGGCGGGTACAAGGGTCGCGTGGGGAT
TTATGAAGTGGTGAAGAACACCCCCGAACTGCAACGACTGATCATGGCCGAGGGCAACTCACTGGAAATCGATATACAGA
TGCGCCGGGACGGCTTCAACGACCTGCGCACCTCGGGCCTCATCAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.407

99.823

0.583
  pilB Acinetobacter baumannii D1279779

57.801

99.647

0.576
  pilB Legionella pneumophila strain ERS1305867

56.36

100

0.564
  pilB Vibrio cholerae strain A1552

50.794

100

0.509
  pilB Vibrio campbellii strain DS40M4

48.264

100

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

49.376

99.117

0.489
  pilF Neisseria gonorrhoeae MS11

48.845

99.47

0.486
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.623

93.64

0.371
  pilF Thermus thermophilus HB27

36.918

98.587

0.364