Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   JZM58_RS03190 Genome accession   NZ_CP071797
Coordinates   700634..702712 (+) Length   692 a.a.
NCBI ID   WP_207984786.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain YK-310     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 695634..707712
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JZM58_RS03170 (JZM58_03170) comM 697848..698912 (+) 1065 WP_207984783.1 pilus assembly protein PilM Machinery gene
  JZM58_RS03175 (JZM58_03175) - 698912..699478 (+) 567 WP_007952387.1 PilN domain-containing protein -
  JZM58_RS03180 (JZM58_03180) pilO 699475..700098 (+) 624 WP_207984784.1 type 4a pilus biogenesis protein PilO -
  JZM58_RS03185 (JZM58_03185) - 700095..700619 (+) 525 WP_207984785.1 pilus assembly protein PilP -
  JZM58_RS03190 (JZM58_03190) pilQ 700634..702712 (+) 2079 WP_207984786.1 type IV pilus secretin PilQ Machinery gene
  JZM58_RS03195 (JZM58_03195) aroK 702717..703235 (+) 519 WP_045121685.1 shikimate kinase AroK -
  JZM58_RS03200 (JZM58_03200) aroB 703386..704486 (+) 1101 WP_011332082.1 3-dehydroquinate synthase -
  JZM58_RS03205 (JZM58_03205) - 704497..706098 (+) 1602 WP_207984787.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 75073.21 Da        Isoelectric Point: 5.5724

>NTDB_id=547824 JZM58_RS03190 WP_207984786.1 700634..702712(+) (pilQ) [Pseudomonas fluorescens strain YK-310]
MNRIFSTLGFSLWMALLSPMVQAANLKALDVAALPGDRVELKLSFDGPPPQPKGYTTDSPARIALDLPGVASQLTSKTRD
LGSGNARTATVVEANNRTRLIISLTQLAPYDTRVEGNTVFVVVGQGSKVAAPRTAARTPRAAPAAAPVRTFAPTARAIRG
VDFQRGTAGEGNVVIDLSDPAIAPDIQEHDGKIILNFARTQLSEPLRVRLDVKDFATPVQFVNAGVSGDRATITVEPSGT
FDYSTYQTDNKLTVSIRPLTVDDLQKRNADRNSYNGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNV
PWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEAK
VDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVSN
GSSTTIGTPGSTSTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=547824 JZM58_RS03190 WP_207984786.1 700634..702712(+) (pilQ) [Pseudomonas fluorescens strain YK-310]
ATGAACAGGATTTTTTCCACCCTCGGTTTTTCGCTATGGATGGCGCTGCTTTCACCGATGGTACAGGCAGCCAATCTGAA
GGCGCTGGACGTCGCGGCACTGCCGGGCGACCGGGTCGAGCTGAAGCTGTCGTTCGACGGTCCGCCCCCGCAGCCCAAGG
GCTACACCACTGATTCGCCAGCGCGGATCGCCCTCGATCTGCCGGGTGTCGCCAGTCAGCTGACGAGCAAGACCCGTGAC
CTGGGCAGTGGCAATGCCCGCACGGCCACGGTGGTCGAAGCCAATAACCGGACGCGGCTGATCATCAGCCTGACGCAACT
GGCGCCTTACGACACCCGGGTCGAAGGCAATACGGTATTCGTGGTGGTCGGGCAAGGCAGCAAAGTCGCTGCCCCACGTA
CGGCGGCTCGTACACCGCGTGCAGCACCTGCGGCAGCGCCGGTCCGCACGTTTGCGCCGACCGCCAGGGCCATTCGCGGT
GTGGATTTCCAGCGTGGCACGGCGGGCGAGGGCAACGTGGTCATCGACCTGTCCGATCCAGCCATCGCCCCGGACATTCA
GGAACACGACGGCAAGATCATCCTCAATTTCGCCCGTACCCAATTGTCCGAGCCGCTGCGGGTGCGCCTCGACGTCAAGG
ATTTCGCCACCCCGGTGCAGTTCGTCAATGCCGGTGTCAGCGGTGATCGCGCGACGATCACCGTCGAACCCAGCGGTACG
TTCGACTATTCCACTTACCAGACCGACAACAAGCTTACTGTCAGTATCCGGCCGTTAACCGTCGATGACCTGCAAAAGCG
CAACGCCGACCGCAACAGCTATAACGGCGAAAAGCTCTCTCTCAACTTCCAGGACATCGATGTGCGCTCTGTGCTGCAAC
TGATCGCCGACTTCACCAACCTCAATCTGGTGGCCAGCGATACGGTGCAGGGCGGCATTACCTTGCGCCTGCAGAACGTG
CCGTGGGATCAGGCGCTGGATCTGGTGCTCAAGACCAAAGGCCTGGACAAACGCAAGGTCGGCAATGTGTTGCTGGTGGC
GCCGGCAGATGAAATCGCCGCCCGCGAACGGCAGGAACTGGAATCGCAGAAACAGATCGCCGAACTGGCGCCGTTGCGCC
GCGAGCTGCTGCAAGTGAACTACGCCAAGGCTGCGGACATCGCAAAGCTGTTCCAGTCGGTCACCAGTGCCGAGGCCAAG
GTCGACGAGCGGGGGTCGATCACCGTCGATGAACGCACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGACGA
ACTGCGGCGGATCGTGGCGCAGCTGGATATTCCGGTGCGTCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCG
ACTACGACAAGAGCCTGGGCGTGCGCTGGGGCGGTTCGATCCAGAACAAGGGCAACTGGAACACTTCCGGGGTCAGCAAC
GGTTCGTCGACCACCATCGGCACGCCGGGCAGCACCAGCACCAACTCGCCGTTCGTCGACATGGGCACGGTCAACAACAC
GTCCGGAATCGGCATCGCATTCATCACCGACAACGTCTTGCTCGACCTTGAGCTGACCGCGATGGAGAAGACCGGCAACG
GCGAAATCGTCTCGCAGCCCAAGGTGGTCACCTCCGACAAGGAAACCGCGAAGATCCTCAAGGGCACCGAGATTCCGTAT
CAGGAAGCCAGCTCCAGCGGCGCTACGTCGGTGTCCTTCAAGGAGGCGTCGCTGTCGCTGGAAGTGACGCCGCAGATCAC
CCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGACTACCTGAACAAAGTGCAGGATGTACCGC
CGATCAAGAAAAACGAGGTCAACGCCAAGGTGCTGGTGAACGACGGCGAGACCATCGTGATCGGGGGCGTTTTCTCAAAT
ACTCAAAGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(302-348)

Bacterial type II/III secretion system short domain (Secretin_N)

(376-441)

AMIN domain (AMIN)

(37-121)

Bacterial type II and III secretion system protein (Secretin)

(527-683)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.469

100

0.738
  comQ Acinetobacter baylyi ADP1

40.358

100

0.423
  pilQ Acinetobacter baumannii strain A118

37.65

100

0.408
  pilQ Acinetobacter baumannii D1279779

37.65

100

0.408
  pilQ Legionella pneumophila strain Lp02

37.666

97.832

0.368
  pilQ Legionella pneumophila strain ERS1305867

37.63

97.543

0.367