Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   J1M35_RS04175 Genome accession   NZ_CP071796
Coordinates   887099..888838 (-) Length   579 a.a.
NCBI ID   WP_208011165.1    Uniprot ID   A0A975CKG6
Organism   Ottowia testudinis strain 27C     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 882099..893838
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J1M35_RS04140 (J1M35_04140) - 882479..882823 (+) 345 WP_208010008.1 IS630 transposase-related protein -
  J1M35_RS04145 (J1M35_04145) - 882750..883328 (+) 579 WP_284144052.1 IS630 family transposase -
  J1M35_RS04150 (J1M35_04150) - 883373..883591 (-) 219 WP_208010010.1 DNA gyrase inhibitor YacG -
  J1M35_RS04155 (J1M35_04155) zapD 883588..884343 (-) 756 WP_208010011.1 cell division protein ZapD -
  J1M35_RS04160 (J1M35_04160) coaE 884369..884983 (-) 615 WP_208010012.1 dephospho-CoA kinase -
  J1M35_RS04165 (J1M35_04165) pilD 884995..885837 (-) 843 WP_208010013.1 A24 family peptidase Machinery gene
  J1M35_RS04170 (J1M35_04170) pilC 885837..887060 (-) 1224 WP_208010014.1 type II secretion system F family protein Machinery gene
  J1M35_RS04175 (J1M35_04175) pilB 887099..888838 (-) 1740 WP_208011165.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J1M35_RS04185 (J1M35_04185) - 889084..890013 (-) 930 WP_208011166.1 polyprenyl synthetase family protein -
  J1M35_RS04190 (J1M35_04190) rplU 890269..890598 (+) 330 WP_208010015.1 50S ribosomal protein L21 -
  J1M35_RS04195 (J1M35_04195) rpmA 890605..890865 (+) 261 WP_208010016.1 50S ribosomal protein L27 -
  J1M35_RS04200 (J1M35_04200) cgtA 890950..892017 (+) 1068 WP_208010017.1 Obg family GTPase CgtA -
  J1M35_RS04205 (J1M35_04205) proB 892072..893217 (+) 1146 WP_208010018.1 glutamate 5-kinase -
  J1M35_RS04210 (J1M35_04210) - 893261..893788 (-) 528 WP_208010019.1 CNP1-like family protein -

Sequence

Protein


Download         Length: 579 a.a.        Molecular weight: 63145.64 Da        Isoelectric Point: 5.2517

>NTDB_id=547806 J1M35_RS04175 WP_208011165.1 887099..888838(-) (pilB) [Ottowia testudinis strain 27C]
MAVADPISKSAVPTALPGVGRALVMAGKISQQTADDVFRKAKAANRNFITELTESGAISASDLAHTMAQAFSAPLLDIDA
LDAQRLPKDLLDPKLSATYRILALSKRGGRLIVVTADPSDHEAAEKIKFATQMGVEWVIGEFNKLSRLIEEHTKTATQAM
DSIVGGDFEFDEASLATDAPAEDTEAPQTDVEDAPVVKFLQKMLLDAFNMRASDLHFEPYEHNYRVRFRIDGELREIVSP
PVAIKEKLASRIKVLSRLDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPIEK
ERLLKAIERPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNDKAGLTFAVALKAFLRQD
PDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCVQCKAPVDI
PRETLLEAGFDELELDGSWTPYKPVGCSACNNGYKGRVGIYEVMPITEELQRIILADGSALEIAEQARREGVRSLRDSGL
QKVKIGVTSLEEVLACTNE

Nucleotide


Download         Length: 1740 bp        

>NTDB_id=547806 J1M35_RS04175 WP_208011165.1 887099..888838(-) (pilB) [Ottowia testudinis strain 27C]
ATGGCCGTTGCTGACCCAATCTCCAAATCCGCCGTACCGACCGCGCTGCCCGGCGTGGGCCGCGCATTGGTCATGGCTGG
CAAGATCAGCCAGCAGACTGCCGATGATGTTTTCCGCAAGGCCAAGGCCGCCAATCGCAATTTCATCACCGAGTTAACCG
AGTCGGGTGCGATTTCCGCGTCGGACCTCGCGCACACGATGGCGCAGGCGTTTTCGGCACCGCTGCTGGACATCGATGCG
CTCGATGCGCAGCGCTTGCCCAAGGACTTGCTCGACCCCAAGCTCTCCGCCACCTACCGCATCCTGGCTTTGAGCAAGCG
CGGCGGTCGATTGATCGTCGTCACGGCAGACCCATCCGACCACGAAGCGGCCGAAAAGATCAAGTTTGCGACGCAGATGG
GCGTCGAGTGGGTCATCGGCGAGTTCAACAAACTCTCGCGCCTGATCGAAGAGCACACCAAGACGGCGACCCAGGCGATG
GACAGCATCGTCGGCGGTGATTTCGAATTCGACGAGGCGTCGCTGGCCACAGACGCGCCGGCCGAGGACACCGAAGCACC
GCAGACCGATGTCGAAGATGCGCCGGTCGTCAAGTTCCTTCAAAAGATGCTGCTCGACGCTTTCAACATGCGGGCGTCTG
ACTTGCACTTCGAGCCCTACGAGCACAACTATCGGGTGCGCTTCCGCATCGACGGCGAGTTGCGCGAGATCGTCTCACCA
CCCGTCGCGATCAAGGAAAAGCTGGCCTCGCGCATCAAGGTGCTGTCGCGCCTGGACATTTCCGAGAAGCGCGTGCCGCA
GGACGGTCGCATGAAGCTGAAGATCGGACCCGACCGCGTGATCGACTTCCGTGTCAGCACCTTACCCACGCTTTTCGGCG
AGAAAATCGTGATCCGTATTCTCGATCCAAGCAGCGCCAAACTGGGCATCGATGCGCTGGGTTACGAGCCGATCGAGAAG
GAGCGCTTGCTCAAGGCCATCGAACGCCCATACGGCATGATCCTGGTGACCGGGCCCACGGGCTCTGGTAAGACCGTATC
GCTCTACACCTGCTTGAACCTGCTGAACAAGCCGGGCGTGAACATCGCGACCGCGGAGGATCCATCCGAAATCAACTTGC
CGGGCGTCAATCAGGTCAACGTCAACGACAAGGCCGGACTGACCTTTGCCGTGGCATTGAAGGCGTTTCTGCGCCAGGAT
CCGGACATCATCATGGTGGGCGAGATTCGCGATCTGGAGACCGCTGACATCTCCATCAAGGCCGCGCAAACCGGTCACCT
GGTATTGTCGACGCTGCACACCAACGACGCGCCGACGACGTTGACGCGCATGCGGAACATGGGCATTGCCCCTTTCAACA
TAGCGTCCAGCGTGATCCTGATCACCGCACAACGCCTGGCCCGGCGGCTGTGTGTGCAATGCAAGGCACCGGTCGACATT
CCGCGCGAAACACTGCTGGAGGCCGGCTTCGATGAATTGGAGCTGGACGGCAGCTGGACGCCTTACAAACCCGTCGGCTG
CTCGGCCTGCAACAACGGCTATAAGGGCCGCGTCGGCATTTACGAGGTCATGCCCATCACCGAAGAACTCCAGCGCATCA
TTCTGGCCGACGGCAGCGCGCTGGAGATCGCAGAGCAGGCGCGGCGCGAAGGCGTGCGCTCGCTGCGCGATTCGGGGCTG
CAAAAAGTCAAGATCGGCGTCACTTCGCTTGAAGAAGTGCTGGCCTGTACCAACGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(202-469)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.36

97.755

0.551
  pilB Acinetobacter baylyi ADP1

54.031

100

0.544
  pilF Neisseria gonorrhoeae MS11

52.389

97.582

0.511
  pilB Legionella pneumophila strain ERS1305867

50.357

96.718

0.487
  pilB Vibrio parahaemolyticus RIMD 2210633

45.307

95.682

0.434
  pilB Vibrio campbellii strain DS40M4

44.821

96.718

0.434
  pilB Vibrio cholerae strain A1552

47.419

90.328

0.428
  pilF Thermus thermophilus HB27

38.821

99.655

0.387
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.977

97.927

0.382