Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   J4G44_RS04000 Genome accession   NZ_CP071766
Coordinates   843709..845421 (-) Length   570 a.a.
NCBI ID   WP_179996326.1    Uniprot ID   -
Organism   Acinetobacter towneri strain GX3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 838709..850421
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4G44_RS03970 (J4G44_03970) - 838743..839066 (+) 324 WP_004975854.1 pyrimidine/purine nucleoside phosphorylase -
  J4G44_RS03975 (J4G44_03975) rlmB 839255..840004 (+) 750 WP_004975856.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  J4G44_RS03980 (J4G44_03980) - 840099..840977 (+) 879 WP_042068576.1 DMT family transporter -
  J4G44_RS03985 (J4G44_03985) coaE 840983..841582 (-) 600 WP_004975860.1 dephospho-CoA kinase -
  J4G44_RS13600 (J4G44_03990) pilD 841592..842452 (-) 861 WP_004975862.1 prepilin peptidase Machinery gene
  J4G44_RS03995 (J4G44_03995) pilC 842452..843678 (-) 1227 WP_004975864.1 type II secretion system F family protein Machinery gene
  J4G44_RS04000 (J4G44_04000) pilB 843709..845421 (-) 1713 WP_179996326.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J4G44_RS04005 (J4G44_04005) tpiA 845699..846490 (+) 792 WP_019836081.1 triose-phosphate isomerase -
  J4G44_RS04010 (J4G44_04010) secG 846504..846833 (+) 330 WP_004975868.1 preprotein translocase subunit SecG -
  J4G44_RS04035 (J4G44_04035) rimP 847785..848309 (+) 525 WP_004975871.1 ribosome maturation factor RimP -
  J4G44_RS04040 (J4G44_04040) nusA 848345..849829 (+) 1485 WP_154321110.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63483.64 Da        Isoelectric Point: 4.8148

>NTDB_id=547594 J4G44_RS04000 WP_179996326.1 843709..845421(-) (pilB) [Acinetobacter towneri strain GX3]
MSVQQATPRFTGFIRRLVEDGLVSAENMQTAIKAAQKDNVDIVPYLIQNLNIAPLIIAEKISNEFGEPIFDLSVYDSALI
VREGIDEKLFSKYNLLPIFKRNHLLYVATSNPTNIEAMDAIRFNSKMNIEAIVVEHDKLVKLIEQNFASESAFEFEDDDF
DLNFEDGNSEPEQAAEDEPQGDESPIVKYINKLLIDAIRMGASDLHFEPYEKTYRVRYRVDGVLRQIATPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKNKSIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPEQKELFMEALEKP
QGMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNNKVGLTFAAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPHCKAPSDIPKQSLLEMGF
TEADLSNPEFQVYQPVGCSECREGYKGRVGVYEVMKVTPEISRIIMEDGNAIQIADASAKSGFNNLRRSGLLKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=547594 J4G44_RS04000 WP_179996326.1 843709..845421(-) (pilB) [Acinetobacter towneri strain GX3]
ATGTCAGTACAACAAGCTACACCTAGATTTACAGGTTTTATTCGACGTTTGGTCGAAGATGGGCTCGTTTCTGCTGAAAA
TATGCAAACTGCCATTAAAGCCGCACAAAAAGATAATGTTGATATTGTTCCCTATCTCATTCAAAACCTAAATATCGCGC
CTTTAATCATTGCTGAAAAAATTTCTAATGAGTTTGGCGAACCTATTTTTGATTTAAGCGTGTATGACTCTGCGCTCATC
GTGCGTGAAGGCATTGATGAAAAGCTTTTCAGCAAATATAACCTTTTACCTATTTTTAAGCGCAATCACCTGCTTTACGT
TGCGACCAGTAACCCGACCAATATCGAAGCAATGGATGCCATTCGTTTCAACAGTAAAATGAATATTGAAGCCATTGTGG
TCGAACACGATAAATTGGTAAAACTGATTGAACAAAACTTTGCGAGTGAGTCTGCTTTTGAATTTGAAGATGATGACTTC
GATCTCAACTTTGAAGATGGTAACAGCGAACCAGAACAAGCTGCAGAAGATGAACCGCAGGGTGATGAATCCCCAATTGT
TAAATACATCAACAAATTACTGATTGATGCAATTCGCATGGGTGCTTCGGACTTACACTTTGAACCGTATGAAAAAACCT
ATCGAGTGCGTTACCGTGTCGATGGTGTATTACGTCAAATTGCCACCCCACCCTTACAGTTAGCTAACCGCTTGGCATCA
CGTTTAAAAGTGATGTCCCAAATGGACATTTCTGAAAAGCGTGTCCCGCAAGATGGTCGTATTAAATTAAAACTATCTAA
AAACAAGTCCATCGACTTCCGTGTGAACTCGTTGCCAACATTGTTCGGTGAAAAAATCGTACTGCGTATTCTTGACCCAT
CTAGCGCGATGCTTGGGATTGACGCTTTGGGCTATGAACCAGAACAAAAAGAATTGTTCATGGAAGCCTTAGAAAAACCG
CAAGGCATGTTACTGATTACAGGCCCAACGGGTTCGGGTAAAACGGTATCGCTATATACAGGCTTAAATATCTTAAACAG
AGAAGATACCAACATTTCCACCGCCGAAGACCCTGTGGAAATTAACTTAGAAGGCATCAATCAGGTTAACGTCAACAATA
AAGTAGGCTTAACCTTCGCTGCCGCCCTGAAATCTTTCTTACGTCAAGACCCTGATATTGTCATGGTGGGTGAGATTCGT
GACCTTGAAACTGCAGAAATTGCAATTAAAGCAGCCCAAACAGGTCACATGGTTTTATCTACCCTCCACACCAACAGTGC
GCCAGAAACTTTAACGCGTTTACGCAATATGGGCGTTCCTTCCTTCAACATCGCAACTTCGGTAAACCTCGTCATTGCAC
AGCGTTTGGCACGACGCCTATGCCCGCATTGCAAAGCTCCTTCCGATATTCCAAAACAAAGCTTATTGGAAATGGGCTTT
ACTGAAGCCGATTTGAGCAATCCAGAATTTCAGGTCTATCAACCTGTAGGCTGTTCTGAATGCCGTGAAGGTTATAAAGG
ACGTGTTGGTGTTTATGAAGTGATGAAAGTCACACCAGAAATTTCTAGAATTATTATGGAGGATGGTAATGCCATCCAAA
TTGCTGATGCGTCAGCAAAATCGGGGTTTAACAATCTGCGTCGTTCGGGGTTACTTAAGGTCATGCAGGGGGTGACTTCA
TTGCAAGAAGTCAATCGTGTGACCAGTGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

78.07

100

0.781
  pilB Acinetobacter baylyi ADP1

76.14

100

0.761
  pilB Legionella pneumophila strain ERS1305867

50.345

100

0.512
  pilF Neisseria gonorrhoeae MS11

49.822

98.596

0.491
  pilB Vibrio cholerae strain A1552

48.587

99.298

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

48.393

98.246

0.475
  pilB Vibrio campbellii strain DS40M4

50.674

91.053

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

34.895

100

0.379
  pilF Thermus thermophilus HB27

37.435

100

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.544

98.596

0.37