Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   J0G10_RS03100 Genome accession   NZ_CP071586
Coordinates   704777..706855 (+) Length   692 a.a.
NCBI ID   WP_220557408.1    Uniprot ID   -
Organism   Pseudomonas germanica strain FIT28     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 699777..711855
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0G10_RS03080 (J0G10_03080) comM 701989..703053 (+) 1065 WP_220557403.1 pilus assembly protein PilM Machinery gene
  J0G10_RS03085 (J0G10_03085) - 703053..703619 (+) 567 WP_095047205.1 PilN domain-containing protein -
  J0G10_RS03090 (J0G10_03090) pilO 703616..704239 (+) 624 WP_095047206.1 type 4a pilus biogenesis protein PilO -
  J0G10_RS03095 (J0G10_03095) - 704236..704763 (+) 528 WP_095047207.1 pilus assembly protein PilP -
  J0G10_RS03100 (J0G10_03100) pilQ 704777..706855 (+) 2079 WP_220557408.1 type IV pilus secretin PilQ family protein Machinery gene
  J0G10_RS03105 (J0G10_03105) aroK 706860..707378 (+) 519 WP_011332081.1 shikimate kinase AroK -
  J0G10_RS03110 (J0G10_03110) aroB 707531..708631 (+) 1101 WP_093430081.1 3-dehydroquinate synthase -
  J0G10_RS03115 (J0G10_03115) - 708642..710231 (+) 1590 WP_095047209.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 75012.58 Da        Isoelectric Point: 5.8300

>NTDB_id=546660 J0G10_RS03100 WP_220557408.1 704777..706855(+) (pilQ) [Pseudomonas germanica strain FIT28]
MNRIFSTLGFSLWIALMSPMVLAANLKMLDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIALDLPGVASQLASKNLD
LGSGNARTATVVEAKERTRLIVSLTQLAPYTTRVEGNNLFVVVGQGAPATAPRPAAAAPRATAKAAASAKAFVPKSRAIR
GVDFQRGTAGEGNVVIDLSDPTIAPDIQEHDGKIILTFARTQLPDKLRVRLDVKDFATPVQFVNAGVTADRTVITVEPSG
TYEYSTFQTDNKLTVSIRPMTVDDLQKRNADRQAYVGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KIDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNASGVN
GSSTTIGTPGSTSTNSPFVDMGTVGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=546660 J0G10_RS03100 WP_220557408.1 704777..706855(+) (pilQ) [Pseudomonas germanica strain FIT28]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGATGTCACCGATGGTACTCGCGGCCAATCTGAA
GATGCTGGACGTGGCGGCGTTGCCGGGTGATCGCGTCGAGCTGAAGTTGTCGTTCGACGGCCCGCCGCCACAACCCAAGG
GCTACACCACCGAATCGCCGGCGCGGATTGCCCTGGACCTGCCCGGTGTCGCCAGTCAGTTGGCCAGCAAGAACCTCGAT
CTGGGCAGTGGCAATGCGCGCACCGCTACTGTGGTTGAGGCCAAGGAACGCACGCGGCTGATTGTCAGCCTGACGCAATT
GGCGCCGTACACCACGCGGGTTGAGGGCAATAACCTGTTCGTGGTGGTCGGTCAGGGCGCACCGGCCACCGCGCCGCGTC
CCGCTGCCGCTGCACCTCGTGCTACCGCTAAAGCTGCTGCGTCCGCCAAGGCTTTTGTGCCGAAAAGCCGGGCGATTCGC
GGTGTGGACTTCCAGCGTGGTACGGCGGGGGAAGGCAATGTGGTTATCGACCTGTCCGACCCGACCATTGCTCCGGATAT
CCAGGAGCATGACGGCAAGATCATCCTCACCTTCGCGCGCACGCAGTTGCCGGACAAGTTGCGGGTACGCCTCGACGTCA
AGGATTTCGCCACCCCGGTGCAGTTCGTCAATGCCGGAGTAACCGCTGATCGGACAGTTATTACGGTTGAACCCAGTGGC
ACCTACGAGTACTCCACCTTCCAGACTGACAACAAACTGACCGTCAGCATCCGCCCGATGACCGTCGATGACCTGCAAAA
ACGCAACGCTGACCGTCAGGCGTACGTCGGCGAAAAGCTCTCGCTGAACTTCCAGGACATTGATGTGCGCTCGGTGCTGC
AACTGATCGCCGACTTCACCAACCTCAATTTGGTTGCCAGCGACACGGTGCAGGGTGGCATCACCTTGCGTCTGCAAAAT
GTGCCGTGGGATCAGGCCCTGGATCTGGTGTTGAAAACCAAAGGCCTGGATAAGCGCAAGATCGGCAATGTGCTGCTGGT
GGCGCCGGCCGATGAAATCGCTGCCCGCGAGCGTCAGGAACTGGAGTCGCAGAAGCAGATCGCCGAACTGGCGCCGTTGC
GCCGCGAGCTGCTACAGGTGAACTACGCTAAAGCCGCGGACATCGCCAAGCTGTTCCAGTCGGTGACCAGTGCCGAGGCG
AAAATCGATGAACGCGGTTCGATTACCGTTGATGAGCGCACCAACAACATCATTGCCTACCAGACTCAGGATCGCCTCGA
CGAACTGCGGCGGATCGTGGCGCAACTGGATATTCCGGTGCGTCAGGTGATGATCGAAGCACGGATTGTCGAGGCCAACG
TCGATTACGACAAAAGTCTGGGCGTGCGCTGGGGCGGCTCGATCCAGAACAAGGGCAACTGGAACGCTTCCGGGGTCAAC
GGTTCATCGACCACCATTGGTACGCCGGGCAGTACCAGCACCAATTCGCCGTTCGTCGACATGGGCACTGTGGGTAATAC
ATCGGGGATCGGCATTGCCTTCATTACCGACAACGTCCTGCTTGATCTTGAGCTGACTGCCATGGAGAAGACCGGCAACG
GCGAAATCGTCTCGCAGCCGAAGGTTGTCACCTCCGACAAGGAAACCGCAAAAATCCTCAAAGGCACCGAGATTCCGTAT
CAGGAAGCCAGCTCCAGCGGCGCGACGTCGGTGTCGTTCAAGGAGGCCTCGCTGTCGCTGGAAGTCACCCCGCAGATCAC
CCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGATTACCTGAACAAAGTGCAGGATGTGCCGC
CGATCAAGAAAAACGAGGTCAACGCCAAGGTACTAGTCAATGACGGCGAAACCATCGTGATTGGCGGTGTTTTCTCAAAT
ACTCAAAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
ATCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACACCACGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(303-349)

AMIN domain (AMIN)

(37-122)

Bacterial type II and III secretion system protein (Secretin)

(527-683)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.222

100

0.759
  comQ Acinetobacter baylyi ADP1

41.643

100

0.418
  pilQ Acinetobacter baumannii strain A118

38.327

100

0.41
  pilQ Acinetobacter baumannii D1279779

38.327

100

0.41
  pilQ Legionella pneumophila strain ERS1305867

38.06

96.821

0.369
  pilQ Legionella pneumophila strain Lp02

38.06

96.821

0.369