Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   J0G10_RS03080 Genome accession   NZ_CP071586
Coordinates   701989..703053 (+) Length   354 a.a.
NCBI ID   WP_220557403.1    Uniprot ID   -
Organism   Pseudomonas germanica strain FIT28     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 696989..708053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0G10_RS03070 (J0G10_03070) - 697736..699004 (+) 1269 WP_090280412.1 malic enzyme-like NAD(P)-binding protein -
  J0G10_RS03075 (J0G10_03075) - 699325..701772 (-) 2448 WP_095047204.1 penicillin-binding protein 1A -
  J0G10_RS03080 (J0G10_03080) comM 701989..703053 (+) 1065 WP_220557403.1 pilus assembly protein PilM Machinery gene
  J0G10_RS03085 (J0G10_03085) - 703053..703619 (+) 567 WP_095047205.1 PilN domain-containing protein -
  J0G10_RS03090 (J0G10_03090) pilO 703616..704239 (+) 624 WP_095047206.1 type 4a pilus biogenesis protein PilO -
  J0G10_RS03095 (J0G10_03095) - 704236..704763 (+) 528 WP_095047207.1 pilus assembly protein PilP -
  J0G10_RS03100 (J0G10_03100) pilQ 704777..706855 (+) 2079 WP_220557408.1 type IV pilus secretin PilQ family protein Machinery gene
  J0G10_RS03105 (J0G10_03105) aroK 706860..707378 (+) 519 WP_011332081.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38093.74 Da        Isoelectric Point: 4.9006

>NTDB_id=546659 J0G10_RS03080 WP_220557403.1 701989..703053(+) (comM) [Pseudomonas germanica strain FIT28]
MLGLFNKKAKTLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPVNAVVEKNIAELEGVGQALSRVLVKARTGLKSVAV
AVAGSAVITKVIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTL
EQAGLAKKQGGLPDDYISEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLSTPTQVANP
FANMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=546659 J0G10_RS03080 WP_220557403.1 701989..703053(+) (comM) [Pseudomonas germanica strain FIT28]
GTGCTGGGACTCTTCAATAAAAAGGCTAAAACGTTACTGGGGATCGACATCAGCTCCACGTCAGTAAAGCTGCTGGAACT
GAGCCGTCAGGGTGACCGCTACCGGGTCGAGGCGTATGCGGTAGAGCCCTTGCCCGTCAACGCCGTGGTCGAAAAGAACA
TCGCCGAGCTTGAAGGTGTCGGTCAGGCGCTGAGCCGTGTGCTGGTCAAGGCGCGCACCGGGCTCAAGAGCGTGGCGGTG
GCAGTGGCCGGTTCCGCCGTGATCACCAAAGTCATCGAAATGGACGCGGGGCTGTCCGACGATGAACTGGAAAACCAGCT
CAAGATCGAAGCCGACCAATATATTCCCTATCCGCTGGATGAAGTTGCCATCGACTTCGAAGTCCAGGGCGTGTCGCCGC
GCAACCCCGAGCGGGTCAACGTGTTGCTGGCCGCCTGTCGCAAGGAAAACGTCGAAGTCCGTGAAGCGGCGCTGGCTCTC
GCCGGGCTGACCGCGCGTGTTGTCGACGTCGAAGCCTATGCGCTCGAGCGCTCGTTCGGCTTGCTCGCAACGCAACTGGC
GGCCTCTCAGGAGCGTCTGACCGTGGCGGTGGTCGACATCGGCGCGACCATGACCACCCTCAGCGTCCTGCACAACGGCA
AGATCATCTACACCCGCGAGCAATTGTTCGGCGGTCGGCAATTGACCGAAGAAATCCAGCGTCGTTATGGCCTGACCCTC
GAGCAGGCAGGGCTGGCGAAAAAGCAGGGCGGTCTGCCCGACGATTACATCAGCGAGGTCCTGCAGCCGTTTCGTGAGGC
GCTGGTCCAGCAGGTTTCACGCTCGTTGCAGTTCTTCTTCGCCTCCGGGCAATACAACGCGGTCGATCACATTCTGCTGG
CCGGCGGCACGGCTTCAGTGCCCGGACTGGATCGGCTGATCGAGCAGCGCCTGAGCACGCCGACTCAAGTAGCCAACCCG
TTTGCCAACATGGCCTTGAGCAGCAAGGTCAACGCCGGGGCTTTGGCCAGCGACGCGCCGGCGCTGATGATCGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

57.062

100

0.571
  pilM Acinetobacter baumannii D1279779

57.062

100

0.571
  comM Acinetobacter baylyi ADP1

55.085

100

0.551
  pilM Legionella pneumophila strain ERS1305867

47.458

100

0.475