Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PPUTUW4_RS01775 Genome accession   NC_019670
Coordinates   386775..388838 (+) Length   687 a.a.
NCBI ID   WP_015093140.1    Uniprot ID   -
Organism   Pseudomonas sp. UW4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 381775..393838
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PPUTUW4_RS01755 (PputUW4_00344) pilM 383987..385051 (+) 1065 WP_015093136.1 pilus assembly protein PilM Machinery gene
  PPUTUW4_RS01760 (PputUW4_00345) - 385051..385617 (+) 567 WP_015093137.1 PilN domain-containing protein -
  PPUTUW4_RS01765 (PputUW4_00346) pilO 385614..386237 (+) 624 WP_015093138.1 type 4a pilus biogenesis protein PilO -
  PPUTUW4_RS01770 (PputUW4_00347) - 386234..386761 (+) 528 WP_015093139.1 pilus assembly protein PilP -
  PPUTUW4_RS01775 (PputUW4_00348) pilQ 386775..388838 (+) 2064 WP_015093140.1 type IV pilus secretin PilQ Machinery gene
  PPUTUW4_RS01780 (PputUW4_00349) aroK 388843..389361 (+) 519 WP_015093141.1 shikimate kinase AroK -
  PPUTUW4_RS01785 (PputUW4_00350) aroB 389512..390612 (+) 1101 WP_015093142.1 3-dehydroquinate synthase -
  PPUTUW4_RS01790 (PputUW4_00351) - 390623..392224 (+) 1602 WP_015093143.1 AAA family ATPase -

Sequence

Protein


Download         Length: 687 a.a.        Molecular weight: 74038.24 Da        Isoelectric Point: 5.3952

>NTDB_id=54611 PPUTUW4_RS01775 WP_015093140.1 386775..388838(+) (pilQ) [Pseudomonas sp. UW4]
MNRIFSTFGLSLWIAMLSPMVQAANLKALDVAALPGDRVELKLSFDAPPPLPQGYMTESPARIALDLPGVVSQLASKTRD
LGSGNARSATVVEAGGRTRLIINLAQSAPYDSRIEGNNLLVVVGQGVKPSAPGPTAPAMPARAAATPGKAIRGVDFQRGT
QGEGNVVIDLSDPSIAPDIQQREGKIIVGFAKTRLPEPLRVRLDVKDFATPVQYVKASASGDRAIISIEPGGAFDYSTYQ
TDSKLTVSVRPATVDDLQKRNAGPQAYSGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQALD
LVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSAEAKPDERGSI
TVDERTNNIIAYQTQDRLDELRRIVTQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNRGNWNTSGVSNGLNGSST
IGTPGSTSTNSPFVDMGVANNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQEASS
SGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSNTQSKV
VDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2064 bp        

>NTDB_id=54611 PPUTUW4_RS01775 WP_015093140.1 386775..388838(+) (pilQ) [Pseudomonas sp. UW4]
ATGAACAGGATTTTCTCCACCTTCGGCCTTTCGCTATGGATAGCGATGTTGTCGCCGATGGTACAAGCGGCCAATCTCAA
GGCGCTGGATGTCGCCGCACTGCCGGGAGACCGGGTCGAGTTGAAGTTGTCGTTCGATGCACCGCCCCCGTTGCCTCAGG
GTTACATGACGGAGTCGCCGGCCCGGATTGCGCTGGATTTGCCGGGTGTCGTCAGTCAGTTGGCCAGCAAGACTCGCGAT
CTCGGTAGTGGAAATGCCCGTAGTGCAACGGTGGTGGAGGCCGGGGGACGTACGCGATTGATCATCAACCTGGCTCAGTC
GGCCCCGTACGACTCGCGAATTGAAGGCAACAATTTGCTGGTGGTTGTCGGGCAGGGTGTCAAACCGTCTGCACCCGGGC
CGACGGCCCCGGCAATGCCGGCACGCGCCGCTGCGACGCCAGGCAAGGCCATTCGGGGTGTGGATTTTCAGCGAGGGACG
CAGGGCGAGGGCAACGTGGTGATCGACTTGTCGGACCCGTCGATTGCGCCCGACATACAGCAGCGCGAGGGCAAGATTAT
TGTCGGGTTCGCCAAAACCCGATTGCCCGAGCCGTTGCGCGTGCGTCTGGATGTGAAGGATTTCGCCACGCCCGTGCAGT
ACGTCAAGGCCAGCGCGAGCGGCGATCGAGCCATCATCAGCATCGAGCCCGGTGGTGCGTTCGATTACTCGACCTATCAG
ACAGACAGCAAGCTCACGGTCAGCGTTCGCCCGGCGACGGTCGACGACCTGCAAAAGCGCAATGCCGGGCCACAGGCTTA
CAGCGGCGAGAAGCTTTCGCTGAACTTCCAGGACATCGATGTGCGGTCGGTGCTGCAATTGATCGCCGATTTCACCAACC
TCAATCTGGTGGCCAGCGACACGGTGCAGGGGGGCATCACGTTGCGCCTGCAGAACGTGCCGTGGGATCAGGCGCTGGAT
CTGGTGCTGAAAACCAAGGGGCTGGACAAGCGCAAGGTCGGTAATGTCCTGCTTGTTGCGCCGGCCGATGAAATTGCTGC
CCGTGAGCGTCAGGAACTGGAATCACAAAAGCAGATTGCCGATCTGGCGCCGTTGCGCCGTGAACTGTTGCAGGTGAATT
ACGCCAAGGCGGCGGACATCGCCAAACTCTTCCAGTCGGTGACCAGTGCCGAAGCGAAACCCGATGAGCGGGGGTCGATC
ACTGTCGACGAGCGCACCAACAACATCATTGCCTACCAGACCCAGGACCGGCTTGATGAGCTGCGCAGGATCGTCACGCA
GCTGGATATCCCTGTGCGTCAGGTCATGATCGAGGCGCGCATTGTCGAAGCCAACGTCGACTATGACAAAAGCCTCGGGG
TGCGTTGGGGCGGATCGATACAGAACCGGGGCAACTGGAACACCTCCGGTGTCAGCAATGGACTCAACGGGTCGTCGACC
ATCGGCACGCCCGGCAGTACCAGCACCAACTCGCCGTTCGTCGACATGGGAGTAGCCAACAACACCTCGGGAATCGGCAT
TGCGTTCATCACCGATAACGTCTTGCTGGACCTGGAACTGACGGCCATGGAGAAAACCGGCAACGGGGAAATCGTCTCGC
AACCCAAGGTGGTCACGTCCGACAAGGAAACCGCCAAGATCCTCAAGGGCACCGAGATTCCCTATCAGGAGGCCAGTTCC
AGTGGCGCGACTTCGGTGTCGTTCAAGGAGGCCTCGCTGTCCTTGGAGGTGACGCCGCAGATCACGCCCGACAACCGGAT
CATCATGGAGGTCAAGGTGACCAAGGACGAGCCGGACTACCTGAACAAGGTCCAGGATGTACCGCCGATCAAGAAAAACG
AGGTCAACGCCAAGGTCCTGGTCAACGATGGCGAGACCATCGTCATTGGCGGCGTTTTCTCAAATACTCAGAGCAAGGTC
GTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGTTTCGGAGAAAAAATC
CGAGCTGTTGGTATTCCTCACTCCGCGTATCATGAATAATCAGGCGATTGCTGTGAGTCATTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(295-341)

Bacterial type II/III secretion system short domain (Secretin_N)

(369-434)

AMIN domain (AMIN)

(37-121)

Bacterial type II and III secretion system protein (Secretin)

(522-678)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.769

100

0.737
  comQ Acinetobacter baylyi ADP1

41.655

100

0.418
  pilQ Acinetobacter baumannii strain A118

38.172

100

0.413
  pilQ Acinetobacter baumannii D1279779

38.172

100

0.413
  pilQ Legionella pneumophila strain Lp02

38.872

98.108

0.381
  pilQ Legionella pneumophila strain ERS1305867

38.724

98.108

0.38

Multiple sequence alignment