Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PPUTUW4_RS01755 Genome accession   NC_019670
Coordinates   383987..385051 (+) Length   354 a.a.
NCBI ID   WP_015093136.1    Uniprot ID   -
Organism   Pseudomonas sp. UW4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 378987..390051
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PPUTUW4_RS01745 (PputUW4_00342) - 379800..381068 (+) 1269 WP_008047852.1 malic enzyme-like NAD(P)-binding protein -
  PPUTUW4_RS01750 (PputUW4_00343) - 381332..383773 (-) 2442 WP_015093135.1 penicillin-binding protein 1A -
  PPUTUW4_RS01755 (PputUW4_00344) pilM 383987..385051 (+) 1065 WP_015093136.1 pilus assembly protein PilM Machinery gene
  PPUTUW4_RS01760 (PputUW4_00345) - 385051..385617 (+) 567 WP_015093137.1 PilN domain-containing protein -
  PPUTUW4_RS01765 (PputUW4_00346) pilO 385614..386237 (+) 624 WP_015093138.1 type 4a pilus biogenesis protein PilO -
  PPUTUW4_RS01770 (PputUW4_00347) - 386234..386761 (+) 528 WP_015093139.1 pilus assembly protein PilP -
  PPUTUW4_RS01775 (PputUW4_00348) pilQ 386775..388838 (+) 2064 WP_015093140.1 type IV pilus secretin PilQ Machinery gene
  PPUTUW4_RS01780 (PputUW4_00349) aroK 388843..389361 (+) 519 WP_015093141.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37768.51 Da        Isoelectric Point: 4.6613

>NTDB_id=54610 PPUTUW4_RS01755 WP_015093136.1 383987..385051(+) (pilM) [Pseudomonas sp. UW4]
MPGLFNKKSSTLLGIDISSTSVKLLELSRQGERFRVEAYAVEPLPVGAVIEKNIADLEGVGLALARALTRARTGLKGVAV
AVAGSAVITKIIEMDAALSEDEMESQLKIEADQYIPYPLDEVAIDFEVQGTCARNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLASRLAVSEEPLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTGEIQRRYGLTL
EQASLAQRKGGLPEDYAGEVLQPFQQALVQQVSRSLQFFFAAGQYSAVDHILLAGGTASVPGLPLLIEQQLGTPTLIANP
FSEMMLGSKVNGAALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=54610 PPUTUW4_RS01755 WP_015093136.1 383987..385051(+) (pilM) [Pseudomonas sp. UW4]
GTGCCAGGACTCTTCAACAAAAAATCCAGTACGCTCCTGGGAATCGATATCAGCTCTACCTCGGTAAAGCTTCTGGAATT
GAGCCGTCAGGGCGAACGCTTCCGGGTCGAGGCTTATGCGGTCGAGCCTTTGCCCGTCGGCGCGGTGATCGAAAAGAACA
TCGCTGACCTCGAGGGCGTGGGGCTGGCCCTCGCGCGCGCGCTGACCAGGGCCAGAACCGGCCTCAAGGGTGTGGCCGTG
GCTGTGGCAGGCTCGGCGGTGATCACCAAGATCATCGAGATGGATGCCGCTCTTTCCGAAGACGAAATGGAAAGCCAGCT
GAAGATCGAGGCGGATCAATATATTCCCTATCCCCTGGATGAAGTCGCGATCGACTTTGAAGTCCAGGGCACCTGCGCGC
GCAACCCGGAGCGGGTCAACGTGCTCCTCGCGGCTTGCCGCAAGGAAAACGTCGAGGTCCGCGAGGCAGCCCTGGCTCTG
GCCGGACTGACCGCTCGCGTAGTCGATGTCGAGGCCTATGCACTGGAGCGTTCATTCGGGTTGCTGGCCAGTCGGCTTGC
GGTTTCCGAGGAACCGTTGACGGTCGCAGTGGTGGATATCGGCGCCACCATGACCACCCTGAGCGTGCTGCACAACGGGC
GGATTATCTACACCCGTGAGCAACTGTTCGGCGGTCGGCAGTTGACCGGGGAAATCCAGCGTCGCTATGGCCTGACGCTG
GAACAGGCGAGTCTGGCGCAAAGGAAGGGCGGCCTGCCGGAAGACTATGCCGGTGAGGTCCTGCAGCCATTTCAACAGGC
GCTGGTGCAACAGGTGTCGCGCTCGTTGCAGTTCTTCTTTGCTGCAGGCCAATACAGCGCCGTCGACCATATCCTGCTGG
CCGGCGGTACCGCGTCGGTCCCGGGGCTGCCCCTTTTGATCGAGCAGCAATTGGGCACGCCGACCCTGATCGCGAACCCT
TTTTCCGAAATGATGCTGGGCAGCAAGGTCAATGGTGCCGCCCTGGCGAGTGATGCCCCGGCGTTGATGATCGCCTGTGG
TCTTGCCCTCAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

54.701

99.153

0.542
  comM Acinetobacter nosocomialis M2

54.701

99.153

0.542
  comM Acinetobacter baylyi ADP1

51.977

100

0.52
  pilM Legionella pneumophila strain ERS1305867

47.458

100

0.475

Multiple sequence alignment