Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   J2N86_RS08755 Genome accession   NZ_CP071527
Coordinates   1968916..1970583 (+) Length   555 a.a.
NCBI ID   WP_252579014.1    Uniprot ID   -
Organism   Legionella lytica strain PCM 2298     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1963916..1975583
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J2N86_RS08735 (J2N86_08750) pepN 1964086..1966809 (+) 2724 WP_252579010.1 aminopeptidase N -
  J2N86_RS08740 (J2N86_08755) - 1967029..1967589 (-) 561 WP_252579011.1 hypoxanthine-guanine phosphoribosyltransferase -
  J2N86_RS08745 (J2N86_08760) - 1967641..1967967 (-) 327 WP_252579012.1 DUF1820 family protein -
  J2N86_RS08750 (J2N86_08765) - 1968052..1968804 (-) 753 WP_252579013.1 methyltransferase domain-containing protein -
  J2N86_RS08755 (J2N86_08770) pilB 1968916..1970583 (+) 1668 WP_252579014.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J2N86_RS08760 (J2N86_08775) pilC 1970587..1971819 (+) 1233 WP_252579015.1 type II secretion system F family protein Machinery gene
  J2N86_RS08765 (J2N86_08780) - 1971833..1972693 (+) 861 WP_252579016.1 A24 family peptidase -
  J2N86_RS08770 (J2N86_08785) - 1972852..1973607 (+) 756 WP_252579017.1 HAD family hydrolase -

Sequence

Protein


Download         Length: 555 a.a.        Molecular weight: 61690.86 Da        Isoelectric Point: 6.6213

>NTDB_id=546006 J2N86_RS08755 WP_252579014.1 1968916..1970583(+) (pilB) [Legionella lytica strain PCM 2298]
MEFNITGQQFALEPLLGKAETAIHSEQATKEKISLTQYLIKNNILSATIIAHTSAQSFGVPILDLDSIELSTLPLSLVDE
KLMLRHSMVPLFCRGTHLYLAAEDPGNHNALKEIQFHTGLNTHAIAVEANKLTAVFAHLLNMRHHQELSTFDTAMHHEEQ
YEFTINPIDNDDDAPIVKFVNQILFKAIQQGASDIHFEPFEKEYRIRYRHDGLLHEVANPPVNLSARITARIKVMAKLDI
SERRLPQDGSFKINQPNKHPIDFRVSTCPTANGEKVVIRILDLDTAKLHIEALGFNPKQHEYFMQSIKRPQGMILVTGPT
GSGKTVSLYAALNLLNTPKVNISTAEDPVEIKITGINQVNINPKVGLNFSKVLRSFLRQDPDIIMVGEIRDLETAEIAVK
AAQTGHLVLSTLHTNSAVESLTRLMNIGIPSFNIVSSITLIIAQRLARKLCEQCKTPRTDLHKSNLLTLGLNETDQIYQS
VGCSQCTDGYRGRIGLFEVLPMTNTLTELIMAGANSLEILKAAQNEGLTSIYQSGIEKVQQGITTLEEVNRVTVE

Nucleotide


Download         Length: 1668 bp        

>NTDB_id=546006 J2N86_RS08755 WP_252579014.1 1968916..1970583(+) (pilB) [Legionella lytica strain PCM 2298]
ATGGAATTTAATATTACTGGTCAACAATTTGCCTTAGAACCGCTTTTAGGTAAAGCAGAAACCGCAATCCATTCTGAGCA
AGCCACTAAAGAAAAAATTTCACTCACTCAATATCTTATCAAAAATAATATCTTATCTGCCACAATAATTGCCCATACAT
CAGCTCAAAGTTTTGGTGTTCCAATACTGGATCTAGATAGTATTGAGCTCAGTACCCTGCCCTTATCATTAGTAGATGAA
AAACTGATGCTTCGCCATTCCATGGTGCCCCTATTTTGCCGTGGAACCCATTTATATCTGGCTGCCGAAGATCCCGGTAA
TCATAATGCCTTAAAAGAAATTCAATTCCATACCGGTTTGAATACCCATGCGATCGCAGTAGAAGCGAATAAATTGACTG
CCGTGTTCGCCCATTTATTAAACATGAGGCATCATCAAGAATTATCTACATTCGATACAGCAATGCATCATGAAGAACAA
TATGAATTTACAATTAATCCAATAGATAACGATGATGATGCCCCTATTGTAAAATTTGTTAATCAAATTTTATTTAAAGC
AATTCAACAAGGGGCTTCCGACATCCATTTTGAGCCCTTTGAAAAAGAATATCGCATTCGCTACCGACATGATGGCCTTT
TACATGAGGTAGCAAATCCACCAGTCAATCTGTCTGCCCGAATTACAGCGCGGATTAAAGTCATGGCGAAATTAGACATT
TCAGAACGTCGTCTCCCTCAAGATGGTAGTTTTAAGATAAATCAACCTAATAAGCACCCCATTGATTTTCGTGTAAGCAC
CTGCCCCACCGCAAACGGGGAAAAAGTGGTTATAAGAATATTGGATCTCGATACAGCTAAATTACACATTGAAGCCTTAG
GATTTAATCCCAAACAACACGAATATTTTATGCAGTCAATAAAACGTCCTCAAGGAATGATCCTGGTTACTGGCCCCACA
GGTAGTGGAAAAACCGTAAGCCTGTATGCAGCCTTAAATCTGCTTAACACACCAAAAGTTAATATCTCTACCGCTGAAGA
TCCCGTTGAAATTAAAATCACCGGCATTAACCAAGTAAATATCAACCCTAAAGTAGGCCTCAACTTCTCCAAGGTTTTAC
GCTCTTTTTTACGACAAGACCCTGATATTATTATGGTTGGTGAAATTCGTGATTTAGAAACCGCTGAAATTGCAGTTAAG
GCAGCACAAACAGGGCATCTCGTCTTATCAACATTGCATACAAATAGTGCAGTAGAATCCCTAACACGCTTAATGAACAT
AGGTATTCCCAGCTTTAATATCGTGAGTTCAATAACCCTTATTATTGCGCAACGTTTAGCACGAAAACTTTGCGAGCAAT
GTAAAACTCCACGAACCGATCTTCACAAATCCAATTTACTAACTTTGGGTCTTAATGAAACAGACCAAATTTATCAATCC
GTAGGCTGCTCTCAATGCACTGACGGATATCGAGGCCGGATAGGATTATTTGAAGTATTACCTATGACAAATACTCTAAC
CGAACTCATTATGGCGGGTGCTAATTCATTAGAAATTTTAAAAGCAGCCCAAAATGAAGGCCTGACTAGCATTTATCAAT
CTGGCATTGAAAAAGTACAGCAAGGAATAACTACCCTAGAGGAGGTCAATCGGGTAACCGTTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(182-449)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

66.132

100

0.668
  pilB Acinetobacter baumannii D1279779

51.005

98.559

0.503
  pilB Acinetobacter baylyi ADP1

49.634

98.378

0.488
  pilB Vibrio parahaemolyticus RIMD 2210633

47.201

96.577

0.456
  pilB Vibrio campbellii strain DS40M4

47.015

96.577

0.454
  pilB Vibrio cholerae strain A1552

46.097

96.937

0.447
  pilF Neisseria gonorrhoeae MS11

45.287

97.477

0.441
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.752

95.315

0.369
  pilF Thermus thermophilus HB27

38.346

95.856

0.368
  pilB Haemophilus influenzae Rd KW20

41.754

86.306

0.36