Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I8J32_RS00905 Genome accession   NZ_CP071518
Coordinates   198149..199843 (+) Length   564 a.a.
NCBI ID   WP_200614482.1    Uniprot ID   A0A975AU38
Organism   Agrilutibacter solisilvae strain R19     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 193149..204843
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I8J32_RS00885 (I8J32_000885) - 193846..195270 (+) 1425 Protein_175 sigma-54-dependent transcriptional regulator -
  I8J32_RS00890 (I8J32_000890) - 195501..196235 (+) 735 WP_200614210.1 hypothetical protein -
  I8J32_RS00895 (I8J32_000895) - 196502..196879 (+) 378 WP_200614212.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  I8J32_RS00900 (I8J32_000900) - 197592..197966 (+) 375 WP_200614214.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  I8J32_RS00905 (I8J32_000905) pilB 198149..199843 (+) 1695 WP_200614482.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I8J32_RS00910 (I8J32_000910) pilC 199849..201105 (+) 1257 WP_200614216.1 type II secretion system F family protein Machinery gene
  I8J32_RS00915 (I8J32_000915) - 201130..201993 (+) 864 WP_200614218.1 A24 family peptidase -
  I8J32_RS00920 (I8J32_000920) - 201990..202334 (+) 345 WP_200614219.1 hypothetical protein -
  I8J32_RS00925 (I8J32_000925) coaE 202392..203021 (+) 630 WP_200614220.1 dephospho-CoA kinase -
  I8J32_RS00930 (I8J32_000930) - 203113..204024 (-) 912 WP_245156462.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 61591.96 Da        Isoelectric Point: 6.2705

>NTDB_id=545905 I8J32_RS00905 WP_200614482.1 198149..199843(+) (pilB) [Agrilutibacter solisilvae strain R19]
MGITGITRRLVLDGALDEHPAREAMAAASTERKPIANYLVEKKLVTPAQMAAANSFEFGVPLFDASVLDPNQSAIKLVSE
ELVRKHNVLPMFKRGNRLFVGLADPTNTQALDEIKFQSNMVVEAILVDEDKIHRMIEQWLATADNLADVGDEEGIENLTA
SGGDDDLAGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYETDYRVRLRIDGILKQVAKVPVKLQARIAARLKV
MAQLDIAEKRVPQDGRIKLNLSKTKQVDFRVSTLPTLFGEKVVLRILDGGAAKLGIEKLGYEADQQKLFVDSVVKPYGMV
LVTGPTGSGKTVSLYTALNILNNEERNISTVEDPVEIRVPGINQVQMNVKRGMTFAAALRSFLRQDPDVIMVGEIRDLET
AEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGVAPFNITSSVTVVIAQRLARRLHDCKREVKLPEHALLAEGFSHEEV
AAGLKIYEAVGCPDCTEGYKGRTGIYQVMPMTETIQAIVLEGGNAMKIAEAAAQSGIRDLRQSALMKVRNGVTSLAEINR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=545905 I8J32_RS00905 WP_200614482.1 198149..199843(+) (pilB) [Agrilutibacter solisilvae strain R19]
ATGGGCATTACCGGGATCACCCGGCGCCTGGTGCTCGACGGCGCGCTGGACGAGCATCCGGCGCGCGAAGCCATGGCTGC
CGCGTCGACCGAGCGCAAGCCGATCGCCAATTACCTGGTCGAGAAGAAACTGGTCACGCCGGCCCAGATGGCCGCGGCCA
ATTCCTTCGAGTTCGGCGTCCCGCTGTTCGACGCCAGCGTCCTCGATCCCAACCAGAGCGCGATCAAGCTGGTCAGCGAG
GAGCTGGTCCGCAAGCACAATGTGTTGCCGATGTTCAAGCGCGGCAACCGCCTCTTCGTCGGCCTTGCCGACCCGACCAA
CACCCAGGCTCTGGACGAGATCAAGTTCCAGAGCAACATGGTGGTCGAGGCGATCCTCGTCGACGAGGACAAGATCCACC
GGATGATCGAGCAGTGGCTGGCCACTGCCGACAACCTCGCCGACGTCGGCGACGAGGAAGGCATCGAGAACCTCACCGCC
TCCGGCGGCGACGATGACCTTGCCGGCGACAGCGGCGTGGACGCCAAGGGCGACGACACGCCGGTCGTGAAGTTCGTCAA
CAAGGTGCTGGTCGACGCCATCCGTCGCGGCGCCTCGGACATCCACTTCGAACCCTACGAAACCGACTACCGGGTGCGCC
TGCGCATCGACGGCATCCTCAAGCAGGTCGCCAAGGTGCCGGTGAAGCTGCAGGCCCGCATCGCCGCGCGCCTGAAGGTG
ATGGCCCAGCTGGACATCGCCGAGAAGCGCGTCCCGCAGGACGGCCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGGT
CGACTTCCGCGTCAGCACCCTGCCCACGCTGTTCGGCGAGAAGGTCGTGCTGCGTATCCTCGACGGCGGCGCGGCCAAGC
TTGGCATCGAGAAGCTGGGCTACGAGGCCGACCAGCAGAAGCTGTTCGTCGACTCGGTGGTCAAGCCCTACGGCATGGTG
CTGGTCACCGGTCCCACGGGCTCAGGCAAGACCGTCTCGCTCTACACCGCGCTCAACATCCTCAACAACGAGGAGCGCAA
CATCTCCACGGTCGAGGACCCGGTCGAAATCCGCGTGCCGGGCATCAACCAGGTGCAGATGAACGTCAAGCGCGGCATGA
CTTTCGCCGCCGCGCTGCGCAGCTTCCTGCGACAGGATCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTGGAAACG
GCGGAGATCGCCATCAAGGCCGCGCAGACCGGCCACATGGTGCTGTCCACCCTGCACACCAACGACGCGCCGCAGACCAT
CGCGCGCCTGATGAACATGGGCGTGGCCCCGTTCAACATCACCTCGTCGGTGACCGTGGTCATCGCCCAGCGCCTGGCGC
GCCGCCTGCACGACTGCAAGCGCGAGGTGAAGCTGCCCGAACACGCGCTGCTGGCCGAGGGCTTCAGCCACGAGGAAGTC
GCCGCGGGCCTGAAGATCTATGAGGCCGTGGGCTGCCCGGACTGCACCGAAGGCTACAAGGGCCGTACCGGCATCTACCA
GGTCATGCCGATGACCGAGACCATCCAGGCCATCGTGCTGGAAGGCGGCAACGCCATGAAGATCGCCGAGGCGGCCGCAC
AGTCCGGCATCCGCGACCTGCGCCAGTCGGCGCTGATGAAAGTCCGCAACGGCGTCACCAGCCTCGCTGAAATCAACCGC
GTCACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.078

100

0.541
  pilB Acinetobacter baylyi ADP1

53.415

100

0.541
  pilB Legionella pneumophila strain ERS1305867

50.877

100

0.514
  pilB Vibrio cholerae strain A1552

47.535

100

0.479
  pilF Neisseria gonorrhoeae MS11

47.55

97.695

0.465
  pilB Vibrio parahaemolyticus RIMD 2210633

47.925

93.972

0.45
  pilB Vibrio campbellii strain DS40M4

49.299

88.475

0.436
  pilB/pilB1 Synechocystis sp. PCC 6803

36.334

100

0.394
  pilF Thermus thermophilus HB27

39.548

94.149

0.372
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.795

94.149

0.365